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Identification and Antibiotic Susceptibility Test of Bacteria from In Vitro Cultures of Pinellia ternata and Tea Plant

반하 및 차나무의 기내배양시 발생하는 세균의 동정 및 항생제 감수성 검정

  • Kim, Haeng-Hoon (National Institute of Agricultural Biotechnology, RDA) ;
  • Cho, Gyu-Taek (National Institute of Agricultural Biotechnology, RDA) ;
  • Yoon, Mun-Sup (National Institute of Agricultural Biotechnology, RDA) ;
  • Yoon, Ju-Won (National Institute of Agricultural Biotechnology, RDA) ;
  • Cho, Eun-Gi (National Institute of Agricultural Biotechnology, RDA)
  • 김행훈 (농업생명공학연구원 유전자원과) ;
  • 조규택 (농업생명공학연구원 유전자원과) ;
  • 윤문섭 (농업생명공학연구원 유전자원과) ;
  • 윤주원 (농업생명공학연구원 유전자원과) ;
  • 조은기 (농업생명공학연구원 유전자원과)
  • Published : 2003.06.01

Abstract

Contamination of bacterial infection is one of serious problems in in vitro culture system of root crops. From the contaminated tubes over 140 of petiole cultures of Pinellia ternata, a medicinal plant, 4 genera 8species 48 strains of bacteria, including Aeromonas and Pseudomonas, were isolated and identified and another 8 strains were not fully identified. Most of them were motile Gram positive bacteria as in common in early stage of in vitro cultures. Six strains of bacteria, 5 of Gram negative, including Enterobacter, and 1 of Gram positive, were identified from the embryonic axes cultures of tea plant. From the susceptibility test to pre-screened 5 antibiotics, all of the bacteria except for 2 species of Pseudomonas were susceptible to cefotaxime 60∼100mg/L. While 60mg/L erythromycin only was effective to Pseudomonas. Combination of erythromycin 20mg/L and cefotaxime 60mg/L totally suppressed the growth of all bacterial strains tested. Susceptibility test of bacteria from tea embryonic axes cultures showed similar results. Combination of erythromycin 35mg/L and cefotaxime 60mg/L was effective to 15 bacterial strains and partially effective to 1 unidentified.

Keywords

References

  1. Barrett C, Cassells AC (1994) An evaluation of antibiotics for the elimination of Xanthomonas campestris pv. pelargonii (Brown) from Pelargonium x domesticum cv. 'Grand Slam' explants in vitro. Plant Cell Tiss Org Cult 36: 169-175 https://doi.org/10.1007/BF00037716
  2. Boas PH,Terzi JM (1987) Big losses due to bacterial contamination can be avoided in mass propagation scheme. Acta Hort 212: 91-93
  3. Cooke DL, Waites WM, Leifert C (1992) Effect of Agrobacterium tumefaciens, Erwinia carotovora, Pseudomonas syringae and Xanthomonas campestris on plant tissue cultures of Aster, Cheiranthus, Delphinium, Iris and Rosa, disease development in vivo as a result of latent infection in vitro. J Plant Dis Protec 99: 469-481
  4. Eapen S, George L (1990) Infuence of phytohormones, carbohydrates, amino acids, growth supplements and antibiotics on somatic embryogenesis and plant regeneration in finger millet. Plant Cell Tiss Org Cult 22: 87-93 https://doi.org/10.1007/BF00043683
  5. Falkiner FR (1988) Strategy for the selection of antibiotics for use against common bacterial pathogens and endophytes of plants. Acta Hart 225: 53-56
  6. Gerhardt P (1981) Manual of methods for general bacteriology. American Society for Microbiology, Washington, DC
  7. Gerhardt P (1994) Methods for general and molecular bacteriology, 2nd edition, American Society for Microbiology, Washington, DC
  8. Gunson HE, Spencer-Phillips PTN (1993) Latent bacterial infections: endophytes as contaminants of micropropagated plants. In: Lumsden PJ, Nicholas JR, Davies WJ, (eds), Physiology, growth and development of plants in culture, Kluwer Academic Publishers, Dordrocht, Holland, pp 379-396
  9. Holford P, Newbury HJ (1992) The effects of antibiotics and their breakdown products on the in vitro growth of Antirrhinum majus. Plant Cell Rep 11: 93-96
  10. Holt JG (1993) Bergey's manual of determinative bacteriology, 9th Edition, Williams&Wilkins Co., Baltimore
  11. Kim HH, Cha YS, Baek HJ, Cho EG, Chae YA, Engelmann F (2002) Cryopreservation of tea (Camellia sinensis L.) seeds and embryonic axes. Cryo-Letters 23: 209-216
  12. Kim HH, Chae YA (1994) Effects of carbon and nitrogen sources, cytokinin and antibiotics on corm form ation in in vitro cultures of Pinellia ternata(Thunb.) Breit. Korean J Breed 26: 276-281
  13. Leifert C, Camotta H, Waites WM (1992) Effects of combinations of antibiotics on micropropagated Clematis, Delphinium, Hosta and Photinia. Plant Cell TIss Org Cult 29: 153-160 https://doi.org/10.1007/BF00033621
  14. Leifert C, Marris CE, Waites WM (1994) Ecology of microbial saprophytes and pathogens in tissue culture and field-grown plants: reasons for contamination problems in vitro. Crit Rev Plant Sci 13(2): 139-183 https://doi.org/10.1080/713608058
  15. Leifert C, Ritchie JY, Waites WM (1991) Contaminants of planttissue and cell cultures. World J Microbiol Biotech 7: 452-469 https://doi.org/10.1007/BF00303371
  16. Leifert C, Waites WM (1992) Bacterial growth in plant tissue cultures. J Appl Bacteriol 72: 460-466 https://doi.org/10.1111/j.1365-2672.1992.tb01859.x
  17. Leifert C, Waites WM, Niclas JR (1989) Bacterial contaminants of microprpagated plant cultures, J Appl Bacteriol 67: 353-361 https://doi.org/10.1111/j.1365-2672.1989.tb02505.x
  18. Long RD,Curtin TF, Cassels AC (1988) An investigation of the efforts of bacterial contaminants on potato nodal cultivars. Acta Hort 225: 83-91
  19. Mathias PJ, Alderson PG, Leakey RRB (1987) Bacterial contamination in tropical hardwood cultures. Acta Hart 212:43-48
  20. Mathias RJ, Boyd LA (1986) Cefotaxime stimulates callus growth, embryogenesis and regeneration in hexaploid bread wheat (Triticum aestivum L). Plant Sci 46: 212-223
  21. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tatacco tissue culture. Physiol Plant 15: 473-497 https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  22. Nakano M, Mii M (1993) Antibiotics stimulate somatic embryogenesis without plant growth regulators in several Dianthus cultivars. J Plant Physiol 141: 721-725 https://doi.org/10.1016/S0176-1617(11)81581-6
  23. Staley JT (1989) Bergey's manual of systematic bacteriology. Williams and Wilkins Co., Baltimore
  24. Young PM, Hutchins AS, Canfield ML (1984) Use of antibiotics to control bacteria in shoot cultures of woody plants. Plant Sci Lett 34: 203-209 https://doi.org/10.1016/0304-4211(84)90143-3