Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Growth Stimulation of In Vitro Shoots by the Post-supplying of Liquid Medium in Anthurium andreanum ′Atlanta′

액체배지 첨가에 의한 Anthurium andreanum ′Atlanta′의 기내생육 촉진

  • Published : 2003.06.01
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Abstract

In order to enhance shoot elongation and rooting of Anthurium andreanum 'Atlanta' in vitro, 15mL of liquid media containing various concentrations of activated charcoal, sucrose and MS salts were added in same vessels after small shoots were induced from the calli on mudium supplemented with 10.0mg/L BA and 0.1mg/L 2.4-D. The post-supplying of 15mL liquid medium containing MS macro and micro elements, 30g/L sucrose and 5.0∼10.0g/L activated charcoal was significantly stimulated the shoot elongation and rooting of regenerated shoots from calli. The medium addition was also resulted in the enhanced soil survival, elongation and rooting of plantlets in cultural soil mixed with perlite and vermiculite(1 : 1)

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References

  1. Anagnostakis SL (1974) Haploid plants from anthers of tobacco-enhancement with charcoal. Planta 115: 281-283 https://doi.org/10.1007/BF00390524
  2. Debergh PC, Maene LJ (1981) A scheme for commercial propagation of ornamental plants by tissue culture. Sci Hort 14: 335-345 https://doi.org/10.1016/0304-4238(81)90047-9
  3. Doman A, Davidson C, Williams C (1978) Establishment of tissue culture grown plants in the green house environment. Proc Fla State Hort Sci 91: 253-237
  4. Fridborg G, Eriksson T (1975) Effects of activated charcoal on growth and morphogenesis in cell cultures. Physiol Plant 34: 306-308 https://doi.org/10.1111/j.1399-3054.1975.tb03843.x
  5. Fridborg G, Pedersen M, Landstran LE, ErikssonT (1978) The effect of activated charcoal on tissue cultures: absorption ofmetabolites inhibiting morphogenesis. Physiol Plant 43: 104-106 https://doi.org/10.1111/j.1399-3054.1978.tb01575.x
  6. Geier T (1990) Anthurium. In: Ammirato PV, Evans DR, Sharp WR Bajaj YPS eds. Handbook of plant call culture. McGraw Hill Publishing Co New York, pp 228-252
  7. Kunisaki JT (1980) In vitro propagation of Anthurium andreanum Lind. Hort Sci 15: 508-509
  8. Maene LJ, Debergh PC (1983) Rooting of tissue cultured plants under in vivo conditions. Acta Hort 131:201-208
  9. Maene LJ, Debergh PC (1985) LIquid medium addition to established tissue cultures to improve elongation and rooting in vitro. Plant Cell Tissue Organ Cult 5: 23-33 https://doi.org/10.1007/BF00033566
  10. Murashige T, Skoog F (1962) A revised medium for rapid growth and biossays wtth tcoacco tissue culture. Physiol Plant 15: 473-497 https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  11. Pierik RLM (1975) Callus multiplication of Anthurium andreanum Lind. in liquid media. Neth. J Agri Sci 23: 199-302
  12. Pierik RML, Steegmans HHM, van der Meys JAJ (1974) Piantlet formation in callus tissues Anthurium andreanum Lind. Sci Hort 2: 193-198 https://doi.org/10.1016/0304-4238(74)90009-0
  13. Piqueras A, Han BH,van Huylenbrceck JM,Debergh PC (1998) Effect of different environmental conditions in vitro on sucrose metabolism and antioxidant enzymatic activities in cultured shoots of Nicoliana tabacum L. Plant Growth Regul 25: 5-10 https://doi.org/10.1023/A:1005961632380
  14. Yu KJ,Peak KY (1995a) Mictropropagation of Anthurium spp. through shoot tip and callus culture. J Kor Soc Hort Sci 36: 684-694
  15. Yu KJ, Paek KY (1995b) Effect of macroelement levels in the media on shoot tip culture of Anthurium spp. and reestablishment of plantlets in soil. J Kor Soc Hort Sci 36: 893-899
  16. Ziv M, Meir G, Halevy A (1981) Hardening camation plants regenerated from shoot tips cultured in vitro. Environ & Exp Bot 21: 423

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