Journal of Food Hygiene and Safety (한국식품위생안전성학회지)

The Korean Society of Food Hygiene and Safety (한국식품위생안전성학회)
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Identification of Gamma-Irradiated Fruits by using Germination Test

발아법을 이용한 과일류의 방사선 조사 여부 확인

  • 강은경 (한국원자력연구소 식품검지실) ;
  • 오경남 (한국원자력연구소 식품검지실) ;
  • 양재승 (한국원자력연구소 식품검지실)
  • Published : 2003.06.01
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Abstract

A germination method was used to detect biological changes in gamma-irradated apple, orange, and lemon at low doses at 0.1, 0.3, 0.5, 0.7, and 1.0 kGy. Ten fruit seeds of each sample were placed on moistened cotton and germinated at 3$0^{\circ}C$ for 7 days. Shoot lengths of all fruits were gradually grown for 7 days, but the growth was signficantly slow down by fifth day. During 7 days of germination, the growth of unirradiated fruits were significantly highter than the irradiated fruits. By examining the gamma-Irradiated fruits in this study, a germination method could be possibly one of the screening test to identify irradiated fruits.

본 연구는 과일류의 방사선 조사 여부를 검지하기 위해서 생물학적 방법인 발아법으로 방사선 조사여부를 확인하였다. 과일류는 사과, 오렌지, 그리고 레몬으로 0.1, 0.3, 0.5. 0.7, 1.0 kGy의 조사선량으로 방사선 조사하고 7일 동안 순의 길이를 측정하여 방사선 조사에 의한 발아 특성을 살펴보았다. 모든 시료는 7일 동안 지속적인 길이 성장을 하였지만, 방사선 조사에 의해서 길이 성장이 억제되어 3일에서 5일부터는 순의 길이에 유의적인 차이를 보이지 않았다. 사과를 제외하고, 오렌지는 5일째 각각 비조사 시료는 0.5 cm 이상 길이를 보여 비조사 시료와 조사 시 구분이 가능하였다. 레몬은 발아 5일째 0.2 cm 이하의 길이를 보여 비조사 시료와 조사시료의 구분이 가능하였다. 또한 오렌지와 레몬는 3일부터 길이 성장정도가 현저히 낮아지고 발아 5일동안 비조사 시료의 성장정도가 가장 큰 것으로 나타났다. 과일류의 방사선 조사여부는 발아 7일 동안 순 길이의 유의적인 길이성장과 비조사 시료와 조사 시료들의 성장정도를 측정함으로써 확인 가능하였다.

Keywords

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