International Journal of Industrial Entomology and Biomaterials

Korean Society of Sericultural Science (한국잠사학회)
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Possible Presence of an Interleukin-6-Like Molecule in the Immunized Bombyx mori L. (Lepidoptera)

  • Kim, Iksoo (Department of Sericulture and Entomology, The National Institute of Agricultural Science ＆ Technology, RDA) ;
  • Lee, Young-Shin (Department of Life Science, Hoseo University) ;
  • Lee, Joon-Ha (Department of Life Science, Hoseo University) ;
  • Kim, Sang-Hyun (Department of Sericulture and Entomology, The National Institute of Agricultural Science ＆ Technology, RDA) ;
  • Kang, Pil-Don (Department of Sericulture and Entomology, The National Institute of Agricultural Science ＆ Technology, RDA) ;
  • Lee, In-Hee (Department of Life Science, Hoseo University) ;
  • Kim, Jin-Won (Department of Sericulture and Entomology, The National Institute of Agricultural Science ＆ Technology, RDA) ;
  • Lee, Heui-Sam (Department of Sericulture and Entomology, The National Institute of Agricultural Science ＆ Technology, RDA) ;
  • Kang, Seok-Woo (Department of Sericulture and Entomology, The National Institute of Agricultural Science ＆ Technology, RDA)
  • Published : 2003.12.01
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Abstract

Cytokines represent an essential part of the innate immune response in mammals. Recently, several studies have reported the presence of cytokine-like activities and molecules in the invertebrates such as echinoderms, tunicates, mollusks and insects. In our serial study, we investigated presence of cytokines in the silkworm, Bombyx mori, infected with several immune inducers. Western blotting analysis using rabbit anti-human cytokines showed the presence of IL-6-like molecule in the hemolymph collected at 8 and 24 hrs after infection with peptidoglycan and oligodeoxynucleotide, and the molecular weight of the proteins was ∼45 kDa. We attempted to isolate the molecule by gel permeation HPLC, anion exchange chromatography, ultra centrifugation, and immuno-dot-blot assay, but until now the effort was not much successful yet. It, however, does not appear that the IL-6-like molecule in the silkworm larvae is a mere experimental artifact happened by Western blotting analysis. Instead, further experiment on this subject probably will provide us more fruitful result as detected in other invertebrates including insects.

Keywords

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