International Journal of Industrial Entomology and Biomaterials

Korean Society of Sericultural Science (한국잠사학회)
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Isolation and Purification of Polyhydroxylated Alkaloids from Silkworm (Bombyx mori L.)

  • Kim, Hyun-Su (Biotopia Co., Ltd.) ;
  • Ko, Hyun-Jung (Department of Biology, The University of Suwon) ;
  • Cho, Yong-Seak (Department of Biology, The University of Suwon) ;
  • Lee, Jae-Yeon (Department of Biology, The University of Suwon) ;
  • Hwang, Kyo-Yeol (Department of Biology, The University of Suwon) ;
  • Kim, Jin-Won (Department of Sericulture and Entomology, The National Institute of Agricultural Science ＆ Technology, RDA) ;
  • Lee, Heui-Sam (Department of Sericulture and Entomology, The National Institute of Agricultural Science ＆ Technology, RDA) ;
  • Kim, Iksoo (Department of Sericulture and Entomology, The National Institute of Agricultural Science ＆ Technology, RDA) ;
  • Ryu, Kang-Sun (Department of Sericulture and Entomology, The National Institute of Agricultural Science ＆ Technology, RDA)
  • Published : 2003.12.01
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Abstract

Several polyhydroxylated alkaloids were isolated from the extracts of freeze-dried silkworm powder, and purified by ion exchange chromatographic analysis. Through the HPLC analysis, we could identify 1-deoxynojirimycin (DNJ) and a kind of calystegin $B_2$ (HS-58) as well as a noble compound (HS-74) from the purified polyhydroxylated alkaloids. In order to know the characteristics of these isolated alkaloids as enzyme inhibitors, glycosidase inhibition activities of these identified alkaloids including other two non-purified alkaloids (SWP 3-1 and SWP 3-2) were investigated.

Keywords

References

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