Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
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Variation of RAPD patterns between Male and Female Genomic DNAs in Dioecious Rumex acetosa L.

자웅이주 식물 수영 (Rumex acetosa L.)에서 암.수에 따른 RAPD pattern의 다양성 분석

  • 김동순 (충남대학교 자연과학대학 생물학과) ;
  • 구달회 (충남대학교 자연과학대학 생물학과) ;
  • 허윤강 (충남대학교 자연과학대학 생물학과) ;
  • 방재욱 (충남대학교 자연과학대학 생물학과)
  • Published : 2003.02.01
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Abstract

The genetic variation of random amplified polymorphic DNA (RAPD) patterns of genomic DNAs was investigated in dioecious plant Rumex acetosa L., which carries different sex chromosome complements in female (2n=12A+XX) and male (2n=12A+XY$_1$Y$_2$). One hundred and twenty random primers consisted of 10-mer were used for PCR amplification. Polymorphic bands were found in 24 primers. Specific bands for female and male were 16 and 18, respectively. Especially, a band of 1,440 bp from the OPC-10 primer was male specific. These sex specific RAPD markers are used to understanding the sex determination mechanism in plants.

암$.$수 개체 사이에 상이한 성염색체 조성을 지니는 자웅 이주 식물인 수영 (Rumex acetsa L.)에서 120개 의 10-mer random primer를 이용하여 RAPD 분석을 수행하였다. 적용한 primer들 중 24개의 primer 에서 34개의 암$.$수 특이 밴드가 관찰되었다 암 개체 특이 밴드는 16개였으며, 수 개체 특이 밴드는 18개로 나타났다. 특히 OPC-10 primer로부터 얻은 1,440 bp인 DNA 단편은 수 개체 특이적으로 나타났다. 본 연구에서 얻어진 성 특이적인 RAPD 마커들은 식물에서 성 결정 메커니즘 구명의 기본 자료로 활용될 수 있을 것이다.

Keywords

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