Journal of Microbiology

  • 제41권3호
  • /
  • Pages.196-201
  • /
  • 2003
  • /
  • 1225-8873(pISSN)
  • /
  • 1976-3794(eISSN)
한국미생물학회 (The Microbiological Society of Korea)
권호 표지

Isolation and Characterization of Bacillus Strains for Biological Control

  • Kim, Han-Soo (Department of Biotechnology, Yonsei University) ;
  • Park, Jiyong (Department of Biotechnology, Yonsei University) ;
  • Cho, Sung-Won (Institute of Life Science, Greenbiotech Co.) ;
  • Park, Kee-Hyun (Institute of Life Science, Greenbiotech Co.) ;
  • Lee, Gung-Pyo (Institute of Natural Science, Seoul Women′s University) ;
  • Ban, Soo-Jung (Division of Bioindustrial Science, College of Life and Environmental Science, Korea University) ;
  • Lee, Chang-Roo (Division of Bioindustrial Science, College of Life and Environmental Science, Korea University) ;
  • Kim, Chung-Sun (Division of Bioindustrial Science, College of Life and Environmental Science, Korea University)
  • 발행 : 2003.09.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

The object of this study was to characterize Bacillus strains GB-017 and GB-0356, which produce antifungal substances, especially for plant pathogens. In addition, this study was undertaken to characterize the culture conditions required for the production of antifungal substances and to document some of the properties of the antifungal substance produced by these soil-isolated strains. Strains GB-0365 and GB-017 were found to be bacillus-shaped, gram-positive and motile, and to inhibit Botrytis cineria, Fusarium sp., Pythium sp., and Rhizoctonia solani. Antagonistic activity was maintained up to pH 9.0, and the antifungal activity was stable to heat at 80$^{\circ}C$ for 1 h. Antifungal substances were separated and purified using ion exchange and adsorption columns including WK-I0(H$\^$＋) (pH 7.0), HP20 column (pH 3.0) and IPA (pH 3.0). and IPA. Its UV absorption spectrum showed major peaks at 231 and 259 nm, corresponding to polyene and lactone. A fast atom bombardment mass spectrum (FAB MS) showed a highest peak at 441 m/z and major peaks at 192, 205, and 370 m/z.

키워드

참고문헌

  1. Soil Biol. Biochem. v.20 Survival and growth of bacteria introduced into soil Acen,M.J.;C.R.Moore;M.Alexander https://doi.org/10.1016/0038-0717(88)90066-1
  2. Appl. Environ. Microbiol. v.62 Biocontrol of Rhizoctonia solani damping-off of tomato with Bacillus subtilis RB14 Asaka,O.;M.Shoda
  3. Pest management at the crossroads Benbrook,C.M.;E.Groth;J.M.Halloran;M.K.Hansen;S.Marquardt
  4. Proceedings of the Beltwide Cotton Production Research Conferences Potential models of action for suppression of root diseases and yield enhancement when using Bacillus subtilis seed inoculants on cotton Brannen,P.M.
  5. Biol. Control. v.7 Safety of microorganisms intended for pest and plant disease control: a framework for scientific evaluation Cook,R.J.;W.L.Bruckart;J.R.Coulson;M.S.Goettel;R.A.Humber;R.D.Lumsden;J.V.Maddox;M.L.McManus;L.Moore;S.F.Meyer;P.C.Jr.Quimby;J.P.Stack;J.L.Vaughn https://doi.org/10.1006/bcon.1996.0102
  6. Agron. J. v.83 Disease enhancement and Kentucky bluegrass quality as influenced by fungicides Dernoeden,P.H.;M.S.McIntosh https://doi.org/10.2134/agronj1991.00021962008300020012x
  7. Trends Biotechnol. v.12 Metabolites of Pseudomonas involved in the biocontrol of plant disease Dowling,D.N.;F.O'Gara https://doi.org/10.1016/0167-7799(94)90091-4
  8. Pest Control with Enhanced Environmental Safety Challenges of pest control with enhanced toxicological and environmental safety Duke,S.O.;J.J.Menn;J.R.Plimmer;Duke,S.O.(ed.);J.J.Menn(ed.);J.R.Plimmer(ed.)
  9. Bioproducts and bioprocesses Dynamic in situfractional of extracelluar proteins produced in a bakers yeast cultivatrion process Effler,W.T.;R.D.Tanner;G.W.Malaney;Fiechter,A.(ed.);H.Okada(ed.);R.D.Tanner(ed.)
  10. Microb. Ecol. v.22 Side effects of propiconazole(Tilt 250 EC) on non-target soil fungi in a field trial compared with natural stress effects Elmholt,S. https://doi.org/10.1007/BF02540216
  11. J. Bacteriol. v.74 The production of antifungal volatiles by Bacillus subtillis Fiddaman,P.J.;S.Rossal https://doi.org/10.1111/j.1365-2672.1993.tb03004.x
  12. Ann. Rev. Phytopathol. v.26 Role of antibiosis in the biocontrol of diseases Fravel,D.R. https://doi.org/10.1146/annurev.py.26.090188.000451
  13. Crit. Rev. Biotechnol. v.10 Microbial fungicides: recent approaches to educidating mechanisms Gutterson,N. https://doi.org/10.3109/07388559009038205
  14. J. Microbiol. v.37 Isolation and identification of an anaerobic dissimilatory Fe(Ⅲ)-reducing bacterium, Shewanella putrefaciens IR-1 Hyun,M.;B.Kim;I.Chang;H.Park;H.Kim;G.Kim;M.Kim;D.Park
  15. Proceedings of the Beltwide Cotton Production Research Conferences Studies on the mode of action of Bacillus subtillis as a biocontrol agent in cotton Kenney,D.S.;C.R.Howell;E.B.Minton
  16. Mycopathologia v.116 Inhibition of some mycotoxigenic fungi by iturin A, a peptidolipid produced by Bacillus subtilis Klich,M.A.;A.R.Lax;J.M.Bland https://doi.org/10.1007/BF00436368
  17. Phytopathology v.74 An approach to stimulate population proliferation Trichoderma species and other potential biocontrol fungi introduced into natural soil Lewis,J.A.;G.C.Papaizas https://doi.org/10.1094/Phyto-74-1240
  18. Appl. Environ. Microbiol. v.44 Fate in model ecosystems of microbial species of potential use in genetic engineering Liang,L.N.;J.L.Sinclair;L.M.Mallory;M.Alexander
  19. Biology and Control of Soil-Borne Plant Pathogens Influence of volatiles of plant origin on soil borne plant pathogens Linderman,R.G.;R.G.Gilbert;Bruehl,G.W.(ed.)
  20. Biotechnol. Bioeng. v.8 Microfloatation: new low gas-flow rate foam separation technique for bacteria and algae Rubin,A.J.;E.A.Cassel;O.Henderson;J.D.Johnson;J.C.Lamb https://doi.org/10.1002/bit.260080112
  21. J. Agric. Sci. v.124 Fungicide resistance: occurrence and management Russell,P.E. https://doi.org/10.1017/S0021859600073275
  22. J. Gen. Microbiol. v.81 Growth of Bacillus substilis and spore germination in soil observed by a fluorescent-antibody technique Siala,A.;T.R.Gray https://doi.org/10.1099/00221287-81-1-191
  23. Appl. Environ. Microbiol. v.60 Biological activities of two fungistatic antibiotics produced by Bacillus cereus UW85 Silo-suh,L.A.;B.J.Lethbridge;S.J.Raffel;H.He;J.Clardy;J.Handesman
  24. Plant disease control: towards environmentally acceptable methods Strange,R.N.
  25. Can. Bot. v.62 Antagonism of isolates of Bacillus subtilis to Phytophthora cactorum Utkhede,R.S. https://doi.org/10.1139/b84-142
  26. Annu. Rev. Phytopathol. v.26 Biological control of soilborne plant pathogens in the shizosphere with bacteria Weller,D.M. https://doi.org/10.1146/annurev.py.26.090188.002115
  27. FEMS Microbiol. Lett. v.30 Bacillus volatiles antagonize cyanobacteria Wright,S.J.L.;R.J.Thompson https://doi.org/10.1111/j.1574-6968.1985.tb01093.x