• Proceedings of the Korean Society of Plant Pathology Conference
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• 1994.06a
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• pp.75-85
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• 1994

Biocontrol is playing a more and more important role in plant disease management. Evidences show that there are optimum prospects for people to apply biocontrol approach to control plant disease or to study the mechanism of antagonism.“The study of Antagonistic Protein of Bacillus spp.to Xanthomonas oryzae pv. oryzae”has been worked in our laboratory since 1986. One hundred and thirty antagonistics bacteria were screened out, most of them belonged to Bacillus spp., and showed very strong inhibitive effect to various plant pathogens. Nine antagonistic proteins (peptides) were purified (P11-I, P11-II, B8, B826-I, B826-II, A30-I, A30-II, G35). Two antagonistic protein related DNA fragments (B826-I, A30-II) were cloned and sequenced. B826-I DNA fragment composed by 905 bp, and it contained two ORF encoding 95, and 54 amino acids, respectively. By using Rifr and Kamr as the selective markers, we found the bacteria could colonize on rice leaf for at least 40 days. In greenhouse the antagonistic bacteria showed certain degree of control efficiency.

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.23
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• pp.10475-10481
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• 2015

Forkhead-box (FOX) transcription factors comprise a large gene family that contains more than 50 members in man. Extensive studies have revealed that they not only have functions in control of growth and development, but also play important roles in different diseases, especially in cancer. However, biological functions for most of the members in the FOX family remain unknown. In the present study, the expression of 39 FOX genes in 48 kinds of cancer was mined from the Gene Expression Atlas database of European Bioinformatics Institute. The analysis results showed that some FOX genes demonstrate overlapping expression in various cancers, which suggests particular biological functions. The pleiotropic features of the FOX genes make them excellent candidates in efforts aimed to give medical treatment for cancers at the genetic level. The results also indicated that different FOX genes may have the synergy or antagonistics effects in the same cancers. The study provides clues for further functional analysis of FOX genes, especially for the pleiotropic biological functions and crosstalk of FOX genes in human cancers.

• Journal of Microbiology
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• v.41 no.3
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• pp.196-201
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• 2003

The object of this study was to characterize Bacillus strains GB-017 and GB-0356, which produce antifungal substances, especially for plant pathogens. In addition, this study was undertaken to characterize the culture conditions required for the production of antifungal substances and to document some of the properties of the antifungal substance produced by these soil-isolated strains. Strains GB-0365 and GB-017 were found to be bacillus-shaped, gram-positive and motile, and to inhibit Botrytis cineria, Fusarium sp., Pythium sp., and Rhizoctonia solani. Antagonistic activity was maintained up to pH 9.0, and the antifungal activity was stable to heat at 80$^{\circ}C$ for 1 h. Antifungal substances were separated and purified using ion exchange and adsorption columns including WK-I0(H$\^$＋) (pH 7.0), HP20 column (pH 3.0) and IPA (pH 3.0). and IPA. Its UV absorption spectrum showed major peaks at 231 and 259 nm, corresponding to polyene and lactone. A fast atom bombardment mass spectrum (FAB MS) showed a highest peak at 441 m/z and major peaks at 192, 205, and 370 m/z.