Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
권호 표지

Growth Responses of seven Intestinal Bacteria Against Phellodendron amurense Root-Derived Materials

  • Kim, Min-Jeong (Research Center for Industrial Development of Biofood Materials and Institute of Agricultural Science and Technology, College of Agriculture, Chonbuk National University) ;
  • Lee, Sang-Hyun (Research Center for Industrial Development of Biofood Materials and Institute of Agricultural Science and Technology, College of Agriculture, Chonbuk National University) ;
  • Cho, Jang-Hee (Research Center for Industrial Development of Biofood Materials and Institute of Agricultural Science and Technology, College of Agriculture, Chonbuk National University) ;
  • Kim, Moo-Key (Research Center for Industrial Development of Biofood Materials and Institute of Agricultural Science and Technology, College of Agriculture, Chonbuk National University) ;
  • Lee, Hoi-Seon (Research Center for Industrial Development of Biofood Materials and Institute of Agricultural Science and Technology, College of Agriculture, Chonbuk National University)
  • Published : 2003.08.01
Download PDF
⟨ Previous Next ⟩

Abstract

The growth responses of Phellodendron amurense root-derived materials against seven intestinal bacteria were examined, using an impregnated paper disk agar diffusion method and spectrometric method under $O_2$-free condition. The biologically active constituent of the P. amurense root extract was characterized as berberine chloride ($C_{20}H_{18}NO_{41}Cl$) using various spectroscopic analyses. The growth responses varied depending on the bacterial strain, chemicals, and dose tested. At 1 mg/disk, berberine chloride strongly inhibited the growth of Clostridium perfringens, and moderately inhibited the growth of Escherichia coli and Streptococcus mutans without any adverse effects on the growth of three lactic acid-bacteria (Bifidobacterium bifidum, B. longum, and Lactobacillus acidophilus). The structure-activity relationship revealed that berberine chloride exhibited more growth-inhibiting activity against C. perfringens, E. coli, and S. mutans than berberine iodide and berberine sulfate. These results, therefore, indicate that the growth-inhibiting activity of the three berberines was much more pronounced as chloridated analogue than iodided and sulphated analogues. As for the morphological effect caused by 1 mg/disk of berberine chloride, most strains of C. perfringens were damaged and killed, indicating that berberine chloride showed a strong inhibition against C. perfringens. As naturally occurring growth-inhibiting agents, the P. amurense root-derived materials described could be useful as a preventive agent against diseases caused by harmful intestinal bacteria such as clostridia.

Keywords

References

  1. Korean J. Ginseng Sci. v.4 Effects of Panax ginseng extract on growth responses of human intestinal bacteria and bacterial metabolism Ahn,Y.J.;M.Kim;T.Kawamura;T.Yamamoto;T.Fujisawa;T.Mitsuoka
  2. Agric. Biol. Chem v.55 Tea polyphenols:selective growth inhibitors of Clostridium spp. Ahn,Y.J.;T.Kawamura;M.Kim;T.Yamamoto;T.Mitsuoka https://doi.org/10.1271/bbb1961.55.1425
  3. Agric. Chem. Biotechnol. v.45 Growth-inhibiting effects of flowers toward beneficial and harmful human intestinal bacteria Baek,B.R.;M.K.Kim;H.S.Lee
  4. Human Intestinal Microflora in Health and Disease Biotransformation of steroids Bokkenheuser,V.D.;J.Winter
  5. J. Clin. Invest. v.3 Alpha toxin from Clostridium perfringens induces proinflammatory changes in endothelial cells Bunting,M.;D.E.Lorant;A.E.Bryant;G.A.Zimmerman;T.M.Mclntyre;D.L.Stevens;S.M.Prescott https://doi.org/10.1172/JCI100101
  6. Food Sci. Biotechnol. v.11 Fatty acid composition of safflower oil and growth-promoting effect of safflower seed extract toward beneficial intestinal bacteria Cho.J.H.;M.K.Kim;H.S.Lee
  7. Fitoterapia v.72 Topical anti-inflammatory activity of some Asian medicinal plants used in dermatological disorders Cuellar,M.J.;R.M.Giner;M.C.Recio;S.Manez;J.L.Rios https://doi.org/10.1016/S0367-326X(00)00305-1
  8. Cancer Res. v.35 Fecal bacteriology of colonic polyp patients and control patients Finegold,S.M.;D.J.Flora;H.R.Attebery;V.L.Sutter
  9. Human Intestinal Microflora in Health and Disease Biochemical pharmacology and toxicology involving the intestinal flora Goldman,P.;D.J.Hentges(ed.)
  10. Gastroenterology v.53 Studies of intestinal microflora. I. effects of diet, age, and periodic sampling on numbers of fecal microorganisms in man Gorbach,S.L.;L.Nahas;P.I.Lerner;L.Weinstein
  11. Pediatrics v.11 A hiterto unrecognized biochemical difference between human milk and cow's milk Gyogy,P.
  12. Human Intestinal Microflora in Health and Disease Role of the intestinal microflora in host defense against infection Hentges,D.J.
  13. Food Technol. v.45 Bifidobacteria:Their potential for use in American dairy products Hughes,D.B.;D.G.Hoover
  14. J. Microbiol . Biotechnol. v.12 Antibacterial adtivity of Pinus densiflora leaf-derived components tower human intestinal bacteria Hwang,Y.H.; H.S.Lee,
  15. Agric. Chem. Biotechnol. v.45 Fatty acid composition of rice bran oil and growth-promoting effect of rice bran extract and rice bran oil on Bifidobacterium and Lactobacillus Hwang,Y.H.;Y.S.Jang;H.S.Lee
  16. J. Microbiol. Biotechnol. v.12 Identification of Clostridium perfringens AB&J and its uptake of bromophenol blue Kim,J.D.;H.Y.An;J.H.Yoon;Y.H.Park;F.Kawai;C.M.Jung;K.H.Kang
  17. Agric. Chem. Biotechnol. v.44 Growth-inhibiting effects of herb plants on human intestinal bacteria Kim,M.K.;B.S.Park;B.S.Kim;H.S.Lee
  18. Agric. Chem. Biotechnol v.44 Growth-inhibiting effects of vegetable extracts on beneficial and harmful human intestinal bacteria Kim,M.K.;M.J.Kim;D.H.Shin;C.G.Song;H.S.Lee
  19. Food Sci. Britechnol v.11 Growth responses of various flower extracts toward human intestinal microflora Kim,M.K.;H.S.Lee
  20. J. Agric. Food Chem. v.46 Growth-inhibiting effects of Cinnamomum cassia bark-derived materials on human intestinal bacteria Lee,H.S.;Y.J.Ahn https://doi.org/10.1021/jf970548y
  21. Estimating the viability of Fifidobacterium longum in Ca-alginate beads against simulated gastroenteric juices v.11 Lee,K.Y.;J.Y.Kim;Y.J.Lee;E.H.Choi;D.H.Shin;T.R.Heo
  22. J. Ethnopharmcol. v.73 Water extract of 1:1 mixture of Phellodendron cortex and Aralia cortex has inhibitory effects on oxidative stress in kidney of diabetic rats Lee,Y.M.;H.Kim;E.K.Hong;B.H.Kang;S.J.Kim https://doi.org/10.1016/S0378-8741(00)00302-0
  23. Cancer Res. v.38 Fecal profiles of anaerobic microflora of large bowel cancer patients and patients with nonhereditary large bowel polyps Mastromarino,A.;B.S.Reddy;E.L.Wynder
  24. Ann. Microboil.(Paris) v.129B Amino acids produced by bifidibacteria and some clostridia Matteuzzi,D.;F.Crociani;O.Emaldi
  25. J. Microbiol. Biotechnol. v.10 Cytotoxicity of shikonin metabolites with biotransformation of human intestinal bacteria Min,B.S.;M.R.Meselhy;M.Hattori;H.M.Kim;Y.H.Kim
  26. The Lactic Acid Bacteria v.Ⅰ The human gastrointestinal tract Mitsuoka,T.;P.Emeritus;B.J.B.(ed.)
  27. Mitsuoka, T. 1984. A Color Atlas of Anaerobic Bacteria. Shobansha, Tokyo, Japan
  28. A Color Atlas of Anaerobic Bacteria Mitsuoka,T.
  29. Can. Inst. Food Sci. Technol. J. v.23 Bifidobacteria and bifidogenic factors Modler,H.W.;R.C.McKellar;M.Yaguchi https://doi.org/10.1016/S0315-5463(90)70197-6
  30. Appl. Microbiol. v.27 Human fecal flora:The normal flora of 20 Japanese-Hawaiians Moore,W.W.C.;L.V.Holdeman
  31. Planta Medica v.60 Principle of the bark Phellodendron amurense to suppress the cellular immune response Mori,H.;M.Fuchigami; N.Inoue;H.Nagai;A.Koda;I.Nishioka https://doi.org/10.1055/s-2006-959529
  32. J. Dairly Sci. v.78 Immune system stimulation by probiotics Perdigon,G.;S,Alvarez;M.Rachid;G.Aguero;N.Gobbato https://doi.org/10.3168/jds.S0022-0302(95)76784-4
  33. Nutr. Res. v.13 Induction of human cytokines by bacteria used in dairy foods Pereyra,B.S.;D.Lemonnier https://doi.org/10.1016/S0271-5317(05)80737-7
  34. Bifidobacteria and Their Role Rasic,J.L.;J.A.Kurmann
  35. Jpn. J. Microbiol. v.12 Growth responses of Bifidobacterium bifidum co coenzyme A, its precusors and carrot extract Yoshioka,M.;S.Yoshioka;Z.Tamura;K.Ohta