Parasites, Hosts and Diseases

The Korea Society for Parasitology and Tropical Medicine (대한기생충학·열대의학회)
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Specific and common antigens of Clonorchis sinensis and Opisthorchis viverrini (Opisthorchidae, Trematoda)

  • Choi, Min-Ho (Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, and Institute of Endemic Diseases, Seoul National University Medical Research Center) ;
  • Ryu, Jin-Sook (Department of Nuclear Medicine, Asan Medical Center, Ulsan University) ;
  • Lee, Me-Jeong (Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, and Institute of Endemic Diseases, Seoul National University Medical Research Center) ;
  • Li, Shun-Yu (Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, and Institute of Endemic Diseases, Seoul National University Medical Research Center) ;
  • Chung, Byung-Suk (Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, and Institute of Endemic Diseases, Seoul National University Medical Research Center) ;
  • Chai, Jong-Yil (Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, and Institute of Endemic Diseases, Seoul National University Medical Research Center) ;
  • Sithithaworn, Paiboon (Department of Parasitology, Faculty of Medicine, Khon Kaen University) ;
  • Tesana, Smarn (Department of Parasitology, Faculty of Medicine, Khon Kaen University) ;
  • Hong, Sung-Tae (Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, and Institute of Endemic Diseases, Seoul National University Medical Research Center)
  • Published : 2003.09.01
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Abstract

The antigenic characterizations and serological reactions of human liver flukes, Clonorchis sinensis and Opisthorchis viverrini, were analyzed by immunoblot. The antigenic profiles of the crude extract of Clonorchis contained major proteins of 8, 26-28, 34-37, 43, and 70 kDa, and those of Opisthorchis 34-37, 43, 70, and 100 kDa. Of these, the 8, 26-28 and 34-37 kDa bands of Clonorchis and the 100 kDa of Opisthorchis were major components of each excretory-secretory antigen. The 8 and 26-28 kDa bands were specific to Clonorchis but the 100 kDa of Opisthorchis cross-reacted with the sera of clonorchiasis, and the 34-37, 70 and 100 kDa bands cross-reacted with sera of other helminthiases. The frequency and intensity of the immunoblot reactions were positively correlated with the intensity of the liver fluke infection.

Keywords

References

  1. Chen M, Lu Y, Hua X, Mott KE (1994) Progress in assessment of morbidity due to Clonorchis sinensis infection: a review of recent literature. Trop Dis Bull 91:R9-R65.
  2. Choi MH, Park IC, Li S, Hong ST (2003) Excretory-secretory antigen is better than crude antigen for the serodiagnosis of clonorchiasis by ELISA. Korean J Parasitol 41: 35-39.
  3. Elkins DB, Sithithaworn P, Haswell-Elkins M, Kaewkes S, Awacharagan P, Wongratanacheewin S (1991) Opisthorchis viverrini: relationships between egg counts, worms recovered and antibody levels within an endemic community in northeast Thailand. Parasitology 102 Pt 2: 283-288.
  4. Garcia LS, Bruckner DA (1997) Diagnostic Medical Parasitology. (3rd ed.). ASM Press, Washington, D.C., USA.
  5. Haswell-Elkins MR, Satarug S, Elkins DB (1992) Opisthorchis viverrini infection in northeast Thailand and its relationship to cholangiocarcinoma. J Gastroenterol Hepatol 7: 538-548.
  6. Hong ST (1988) Changes of anti-Clonorchis sinensis IgG antibody in serum after praziquantel treatment in human clonorchiasis. Korean J Parasitol 26: 1-8.
  7. Hong ST, Kho WG, Lee M, Lee JS, Lee SH (1997) Immunoblot patterns of clonorchiasis. Korean J Parasitol 35: 87-93.
  8. Hong ST, Lee M, Sung NJ, Cho SR, Chai JY, Lee SH (1999) Usefulness of IgG4 subclass antibodies for diagnosis of human clonorchiasis. Korean J Parasitol 37: 243-248.
  9. Kim SI (1998) A Clonorchis sinensis-specific antigen that detects active human clonorchiasis. Korean J Parasitol 36: 37-45.
  10. Lee SH, Hwang SW, Chai JY, Seo BS (1984) Comparative morphology of eggs of heterophyids and Clonorchis sinensis causing human infections in Korea. Korean J Parasitol 22: 171-179.
  11. Rim HJ (1986) Current pathobiology and chemotherapy of clonorchiasis. Korean J Parasitol 24(Supplement): 1-141.
  12. Sirisinha S, Sahassananda D, Bunnag D, Rim HJ (1990) Immunological analysis of Opisthorchis and Clonorchis antigens. J Helminthol 64: 133-138.
  13. Sithithaworn P, Haswell-Elkins MR, Mairiang P, et al. (1994) Parasite-associated morbidity: liver fluke infection and bile duct cancer in northeast Thailand. Int J Parasitol 24:833-843.
  14. Sithithaworn P, Tesana S, Pipitgool V, et al. (1991) Relationship between faecal egg count and worm burden of Opisthorchis viverrini in human autopsy cases. Parasitology 102 Pt 2: 277-281.
  15. Yong TS, Im KI, Chung PR (1991) Analysis of Clonorchis sinensis antigens and diagnosis of clonorchiasis using monoclonal antibodies. Korean J Parasitol 29: 293-310.