Parasites, Hosts and Diseases

The Korea Society for Parasitology and Tropical Medicine (대한기생충학·열대의학회)
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Laboratory passage and characterization of an isolate of Toxoplasma gondii from an ocular patient in Korea

  • Chai, Jong-Yil (Department of Parasitology and Tropical Medicine, Seoul National University Medical Research Center) ;
  • Lin, Aifen (Department of Parasitology and Tropical Medicine, Seoul National University Medical Research Center) ;
  • Shin, Eun-Hee (Department of Parasitology and Tropical Medicine, Seoul National University Medical Research Center) ;
  • Oh, Myoung-Don (Department of Internal Medicine, Seoul National University College of Medicine, and Institute of Endemic Diseases, Seoul National University Medical Research Center) ;
  • Han, Eun-Taek (Department of Parasitology, Kangwon National University College of Medicine) ;
  • Nam, Ho-Woo (Department of Parasitology and Catholic Institute of Parasitic Diseases, College of Medicine, Catholic University of Korea) ;
  • Lee, Soon-Hyung (Department of Parasitology and Tropical Medicine, Seoul National University Medical Research Center)
  • Published : 2003.09.01
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Abstract

Toxoplasma gondii tachyzoites were isolated from the blood of an ocular patient, and have been successfully passaged in the laboratory, for over a year, by peritoneal inoculation in mice. The isolated parasite was designated the Korean Isolate-1 (KI-1) and its characteristics were compared with those of the RH strain, a well-known virulent strain originating from a child who suffered from encephalitis. The morphology, pathogenicity, infectivity and cell culture characteristics of the KI-1 were similar to those of the RH strain. Both RH and KI-1 antigens were detected by an anti-T gondii monoclonal antibody (mAb), Tg563, against the major surface protein SAG1 (30 kDa), whereas no reaction was observed against an anti-Neospora caninum mAb, 12B4. The KI-1 was confirmed as an isolate of T. gondii. A long-term laboratory maintenance and characterization of a local T gondii isolate is reported for the first time in the Republic of Korea.

Keywords

References

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