Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
권호 표지

HpkA, a Histidine Protein Kinase Homolog, is Required for Fruiting Body Development in Myxococcus xanthus

  • Park, Sooyeon (Section of Life Science, Hoseo University) ;
  • Kim, Jihoon (Section of Life Science, Hoseo University) ;
  • Lee, Bongsoo (Section of Life Science, Hoseo University) ;
  • Zusman, David R (Section of Life Science, Hoseo University) ;
  • Cho, Kyungyun (Section of Life Science, Hoseo University)
  • Published : 2003.06.01
Download PDF
⟨ Previous Next ⟩

Abstract

A gene (hpkA), encoding a histidine protein kinase homolog, has been identified in the upstream region of the espAB operon in Myxococcus xanthus. It encodes a 333 amino acid (35,952 Da) protein with a histidine protein kinase domain in the region from amino acid 90 to 317. Null mutations in the hpkA gene caused formation of loose irregular fruiting bodies, while wild-type strains developed tight hemispherical fruiting bodies under developmental conditions. Sporulation of the hpkA mutant was delayed by at least 12 h compared to that of the wild-type. It appeared that the hpkA mutation increased the expression of the espAB operon by more than 2-fold compared with the wild-type under developmental conditions. Expression of the hpkA gene was low under vegetative conditions, but was highly induced under developmental conditions.

Keywords

References

  1. Nucleic Acids Res. v.25 Gapped database search programs Altschul,S.F.;T.L.Madden;A.A.Schaffer;J.Zhang;Z.Zhang;W.Miller;D.J.Lipman https://doi.org/10.1093/nar/25.17.3389
  2. Proc. Natl. Acad. Sci. USA v.72 Regulation of development in Myxococcus xanthus: Effect of 3',5'-cyclic AMP, ADP, and nutrition Campos,J.M.;D.R.Zusman https://doi.org/10.1073/pnas.72.2.518
  3. Mol. Microbiol. v.34 AsgD, a new two-component regulator required for A-signalling and nutrient sensing during early development of Myxococcus xanthus Cho,K.;D.R.Zusman https://doi.org/10.1046/j.1365-2958.1999.01594.x
  4. Mol. Microbiol. v.34 Sporulation timing in Myxococcux xanthus is controlled by the espAB locus Cho,K.;D.R.Zusman https://doi.org/10.1046/j.1365-2958.1999.01633.x
  5. J. Microbiol. Biotechnol. v.12 Identification and functional analysis of the putAP genes encoding Vibrio vulnificus proline dehydrogenase and proline permease Kim,H.J.;J.H.Lee;J.E.Rhee;H.S.Jeong;H.K.Choi;H.J.Chung;S.Ryu;S.H.Choi
  6. Myxobacteria Ⅱ Dworkin,M.;D.Kaiser
  7. J. Gen. Microbiol. v.137 Induction of germination in Myxococcus xanthus fruiting body spores Elias,M.;F.J.Murillo https://doi.org/10.1099/00221287-137-2-389
  8. Modern Microbial Genetics(2nd ed.) Genetic tools for dissecting motility and development of Myxococcus xanthus Hartzell,P.;U.N.Streips(ed.);R.E.Yasbin(ed.)
  9. J. Microbiol. Biotechnol. v.12 Utilization of lacZ to isolate regulatory genes from Corynebacterium glutamicum Kim,H.J.;J.S.Park;H.S.Lee;Y.Kim
  10. J. Bacteriol. v.183 Myxococcus xanthus mokA encodes a histidine kinase-response regulator hybrid sensor required for development and osmotic tolerance Kimura,Y.;H.Nakano;H.Terasaka;K.Takegawa https://doi.org/10.1128/JB.183.4.1140-1146.2001
  11. Dev. Biol. v.117 A global analysis of developmentally regulated genes in Myxococcus xanthus Kroos,L;A.Kuspa;D.Kaiser https://doi.org/10.1016/0012-1606(86)90368-4
  12. Mol. Microbiol v.40 C-signal: A cell surface-associated morphogen that induces and co-ordinates multicellular fruiting body morphogenesis and sporulation in Myxococcus xanthus Kruse,T.;S.Lobedanz;N.M.Berthelsen;L. Sogaard-Andersen https://doi.org/10.1046/j.1365-2958.2001.02365.x
  13. J. Bacteriol. v.179 Methods for generating precise deletions and insertions in the genome of wild-type Escherichia coli: Application to open reading frame characterization Link,A.J.;D.Phillips;G.M.Church
  14. J. Bacteriol. v.140 Myxococcus xanthus mutants with temperature-sensitive, stage-specific defects: Evidence for independent pathways in development Morrison,C.E.;D.R.Zusman
  15. EMBNEW NEWS v.4 GeneDoc: Analysis and visualization of genetic variation Nicholas,K.B.;H.B.Nicholas, Jr.;D.W.Deerfield. Ⅱ.
  16. Annu. Rev. Genet. v.26 Communication modules in bacterial signaling proteins Parkinson,J.S.;E.C.Kofoid https://doi.org/10.1146/annurev.ge.26.120192.000443
  17. J. Bacteriol. v.171 Characterization of the gene for a protein kinase which phosphorylates the sporulation-regulatory proteins Spo0A and Spo0F of Bacillus subtilis Perego,M.;S.P.Cole;D.Burbulys;K.Trach;J.A.Hoch
  18. J. Bacteriol. v.177 The Myxococcus xanthus asgA gene encodes a novel signal transduction protein required for multicellular development Plamann,L.;Y.Li;B.Cantwell;J.Mayor
  19. Environ. Microbiol. v.1 The ecology of the myxobacteria Reichenbach,H. https://doi.org/10.1046/j.1462-2920.1999.00016.x
  20. Molecular Cloning: A Laboratory Manual(2nd ed.) Sambrook,J.;E.F.Fritsch;T.Maniatis
  21. Nucleic Acids Res. v.28 SMART: A web-based tool for the study of genetically mobile domains Schultz,J.;R.R.Copley;T.Doerks;C.P.Ponting;P.Bork https://doi.org/10.1093/nar/28.1.231
  22. Methods Mol. Genet. v.3 Motility and chemotaxis in Myxococcus xanthus Shi,W.;T.Kohler;D.R.Zusman
  23. Prokaryotic Development Developmental aggregation and fruiting body formation in the gliding bacterium Myxococcus xanthus Ward,M.J.;D.R.Zuxman;Y.V.Brun(ed.);L.J.Shimkets(ed.)
  24. Myxobacteria Ⅱ Myxospores and fruiting body morphogenesis White,D.;M.Dworkin(ed.);D.Kaiser(ed.)