Biotechnology and Bioprocess Engineering:BBE

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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The Selective Visualization of Lignin Peroxidase, Manganese Peroxidase and Laccase, Produced by White Rot Fungi on Solid Media

  • Ryu, Won-Youl (School of Chemical Engineering & Technology, Yeungnam University) ;
  • Jang, Moon-Yup (School of Chemical Engineering & Technology, Yeungnam University) ;
  • Cho, Moo-Hwan (School of Chemical Engineering & Technology, Yeungnam University)
  • Published : 2003.04.01
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Abstract

A visual method for the selective screen Eng of lignin degrading enzymes, produced by white rot fungi (WRF), was investigated by the addition of coloring additives to solid media. Of the additives used in the enzyme production media, guaiacol and RBBR could be used for the detection of lignin peroxidase (LiP), manganese peroxidase (MnP) and lactase. Syringaldazine and Acid Red 264 were able for the detection of both the MnP and lactase, and the LiP and laccase, respectively, and a combination of these two additives was able to detect each of the ligninases produced by the WRF on solid media.

Keywords

References

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