Biotechnology and Bioprocess Engineering:BBE

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Transglycosylation Reaction and Raw Starch Hydrolysis by Novel Carbohydrolase from Lipomyces starkeyi

  • Lee, Jin-Ha (Engineering Research Institute, Chonnam National University) ;
  • Lee, Sun-Ok (Department of Material and Biochemical Engineering, Chonnam National University) ;
  • Lee, Gwang-Ok (Department of Material and Biochemical Engineering, Chonnam National University) ;
  • Seo, Eun-Seong (Department of Material and Biochemical Engineering, Chonnam National University) ;
  • Chang, Suk-Sang (Pohang Accelerator Laboratory, Pohang University of Science and Technology) ;
  • Yoo, Sun-Kyun (Department of Food and Biotechnology, Joong Bu University) ;
  • Kim, Do-Won (Department of Physics, Kangnung National University) ;
  • Donal F. Day (Audubon Sugar Institute, Louisiana State University) ;
  • Kim, Doman (Engineering Research Institute, Faculty of Applied Chemical Engineering and Research Institute for Catalysis, Chonnam National University)
  • Published : 2003.04.01
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Abstract

A novel carbohydrolase, which is a DXAMase, containing both dextranase and amylase equivalent activities, was purified from Lipomyces starkeyi KSM22. The purified DXAMase was also found to hydrolyze cellobiose, gentiobiose, trehalose and melezitose, while disproportionation reactions were exhibited with various di- and tri-saccharides, such as maltose, isomaltose, gentiobiose, kojibiose, sophorose, panose, maltotriose, and isomaltotriose with various kinds of oligosaccharides produced as acceptor reaction products. Furthermore, the purified DXAMase hydrolyzed raw waxy rice Starch and produced maltodextrin to the extent of 50% as a glucose equivalent.

Keywords

References

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