Journal of fish pathology (한국어병학회지)

The Korean Society of Fish Pathology (한국어병학회)
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Establishment and characterization ofnew cell line derived from black seabream (Acanthopagrus schlegeli)

감성돔(Acanthopagrus schlegeli) 유래의 주화세포의 확립과 확립된 세포의 특성

  • Im, Eun-Young (Department of Aqualife Medicine, Yosu National University) ;
  • Kang, Min-Sue (Department of Aqualife Medicine, Yosu National University) ;
  • Oh, Myung-Joo (Department of Aqualife Medicine, Yosu National University) ;
  • Jung, Tae-Sung (College of Veterinary Medicine, Gyeongsang National University) ;
  • Jung, Sung-Ju (Department of Aqualife Medicine, Yosu National University)
  • 임은영 (여수대학교 수산생명의학과) ;
  • 강민수 (여수대학교 수산생명의학과) ;
  • 오명주 (여수대학교 수산생명의학과) ;
  • 정태성 (경상대학교 수의학과) ;
  • 정성주 (여수대학교 수산생명의학과)
  • Published : 20031200
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Abstract

A stable cell line, BSBS (black seabream spleen), was established from the cells in spleen of black seabream, Acanthopagrus schlegeli, and characterized. Subculture maintained more than 60 passages and mophologically, BSBS cell was epithelioid cell. The cells grew optimally at 20℃ in Leibovitz's L-15 medium supplemented with 10% fetal bovine serum with incubation temperature of 20℃. BSBS cells supported the growth of marine birnavirus (MABV Y-6), chum salmon reovirus (CSV), spring viremia of carp virus (SVCV) and hirame rhabdovirus (HIRRV). Thus, the new cell line may be useful for studying wide range of fish viruses.

감성돔 (Acanthopagrus schlegeli)의 비장에서 주화세포인 BSBS를 확립하고 세포의 특성을 검사하였다. BSBS세포는 60회 이상의 계대배양이 가능하였고 형태학적으로는 상피성 세포였다. 세포는 20℃, 10%의 FBS가 든 L-15배지에서 배양하는 조건에서 잘 자랐다. BSBS세포는 해양버나바이러스 (MABV Y-6), 잉어의 봄바이러스병바이러스 (SVCV), 넙치의 랍도바이스 (HIRRV)와 연어바이러스 (CSV)를 접종했을 때 세포변성효과가 나타났다. 새로 확립된 주화세포는 앞으로 많은 바이러스병의 연구에 유용하게 사용할 수 있을 것으로 생각된다.

Keywords

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