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생화학분자생물학회 (Korean Society for Biochemistry and Molecular Biology)
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On-off Regulation of 3' Exonuclease Excision to DNA Polymerization by Exo+ Polymerase

  • Zhang, Jia (Genomapping Inc.) ;
  • Li, Kai (Institute of Life Science and Biotechnology, Jinan University)
  • 투고 : 2003.02.07
  • 심사 : 2003.03.04
  • 발행 : 2003.11.30
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초록

The role of 3' exonuclease excision in DNA polymerization was evaluated in primer extensions using 3' allele-specific primers that had exonuclease-digestible and exonuclease-resistant 3' termini. With exonuclease-digestible unmodified 3' mismatched primers, the exo+ polymerase yielded template-dependent products. Using exonuclease-resistant 3' mismatched primers, no primer-extended product resulted from exo+ polymerase. As a control, polymerase without proofreading activity yielded primer-dependent products from 3' mismatched primers. These data indicated that a successful removal of the mismatch is required for DNA polymerization from the 3' mismatched primers by exo+ polymerase. In addition to the well-known proofreading from this mismatch removal, the premature termination in DNA polymerization, due to the failure of the efficient removal of the mismatched nucleotides, worked as an off-switch in maintaining the high fidelity in DNA replication from exo+ polymerase.

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참고문헌

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피인용 문헌

  1. Proofreading genotyping assays mediated by high fidelity exo+ DNA polymerases vol.23, pp.2, 2005, https://doi.org/10.1016/j.tibtech.2004.12.009
  2. Molecular diagnosis of HIV and relevant novel technologies in mutation analysis vol.26, pp.5, 2008, https://doi.org/10.1016/j.biotechadv.2008.04.007
  3. Discrimination of A1555G and C1494T Point Mutations in the Mitochondrial 12S rRNA Gene by On/Off Switch vol.166, pp.1, 2012, https://doi.org/10.1007/s12010-011-9419-4
  4. Exo+ proofreading polymerases mediate genetic analysis and its application in biomedical studies1 vol.26, pp.3, 2005, https://doi.org/10.1111/j.1745-7254.2005.00056.x