• BMB Reports
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• v.38 no.1
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• pp.24-27
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• 2005

The role of 3' exonuclease excision in DNA polymerization was evaluated for primer extension using inert allele specific primers with exonuclease-digestible ddNMP at their 3' termini. Efficient primer extension was observed in amplicons where the inert allele specific primers and their corresponding templates were mismatched. However, no primer-extended products were yielded by matched amplicons with inert primers. As a control, polymerase without proofreading activity failed to yield primer extended products from inert primers regardless of whether the primers and templates were matched or mismatched. These data indicated that activation was undertaken for the inert allele specific primers through mismatch proofreading. Complementary to our previously developed SNP-operated on/off switch, in which DNA polymerization only occurs in matched amplicon, this new mutation detection assay mediated by $exo^+$ DNA polymerases has immediate applications in SNP analysis independently or in combination of the two assays.

• Journal of KIISE:Software and Applications
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• v.33 no.2
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• pp.231-242
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• 2006

Accurate proofreading Interface requires resolving the ambiguity that occurs when the system determines the relations between the free-form by the user and editing scopes of the document. Proofreading for structure documents such as XML/XHTML involves modification of document structures and modified document also should follow the pre-defined DTD. This paper present a CPI (Context-based Proofreading Interface) based on the XML. The CPI supports free-form drawing of correction marks and provides context-based scope recognition and revision methods. CPI provides both implicit and explicit modification methods for document structures. As a result, the correction mark information produced in this paper includes more accurate scope information than that in other systems.

• Journal of Life Science
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• v.29 no.8
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• pp.916-921
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• 2019

In this study, we constructed a biological system that exhibited thermotolerance, ethanol tolerance, and increased ethanol productivity using a random mutagenesis method. We attempted to isolate a thermotolerant mutant using proofreading-deficient DNA polymerase ${\delta}$ and ${\varepsilon}$ encoded by the pol3 and pol2 genes, respectively, in Saccharomyces cerevisiae. To obtain mutants that could grow at high temperatures ($38^{\circ}C$ and $40^{\circ}C$), random mutagenesis of AMY410 (pol2-4) and AMY126 (pol3-01) strains was induced. The parental strains (AMY410 and AMY126) grew poorly at temperatures higher than $38^{\circ}C$. By stepwise elevation of the incubation temperature, AMY410-Ht (heat tolerance) and AMY126-Ht strains that proliferated at $40^{\circ}C$ were obtained. These strains were further incubated in medium containing 6% and 8% ethanol and then AMY410-HEt (heat and ethanol tolerance) and AMY126-HEt strain with ethanol tolerance at an 8% ethanol concentration was obtained. The AMY126-HEt strain grew even at an ethanol concentration of 10%. Furthermore, following the addition of high concentrations of glucose (5% and 10%), an AMY126-HEt3 strain with increased ethanol productivity was isolated. This strain produced 24.7 g/l of ethanol (95% theoretical conversion yield) from 50 g/l of glucose. The findings demonstrate that a new biological system (yeast strain) showing various phenotypes can be easily and efficiently bred by random mutagenesis of a proofreading- deficient mutant.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2007.06a
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• pp.83-86
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• 2007

The necessity of the high speed exclusive use server that connect digital measure instrument flag to internet and CPU use rate of measure flag uses other imbedded operating system with many general servers to verification & proofreading system in remotion. This research is objective that embody high speed network module for Site-Based remote verification & proofreading system and through internet digital measure instrument flag to remote verification & proofreading, TCP/IP Offload Engine processing and improved that is Imbedded TCP/IP stack for high speed networking.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.11 no.6
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• pp.1077-1082
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• 2007

The necessity of the high speed exclusive use server that connect digital measure instrument flag to internet and CPU use rate of measure flag uses other imbedded operating system with many general servers to verification & proofreading system in remotion. This research is objective that embody high speed network module for Site-Based remote verification & proofreading system and through internet digital measure instrument flag to remote verification & proofreading, TCP/IP Offload Engine processing and improved that is Imbedded TCP/IP stack for high speed networking.

• BMB Reports
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• v.36 no.6
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• pp.525-528
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• 2003

The role of 3' exonuclease excision in DNA polymerization was evaluated in primer extensions using 3' allele-specific primers that had exonuclease-digestible and exonuclease-resistant 3' termini. With exonuclease-digestible unmodified 3' mismatched primers, the exo+ polymerase yielded template-dependent products. Using exonuclease-resistant 3' mismatched primers, no primer-extended product resulted from exo+ polymerase. As a control, polymerase without proofreading activity yielded primer-dependent products from 3' mismatched primers. These data indicated that a successful removal of the mismatch is required for DNA polymerization from the 3' mismatched primers by exo+ polymerase. In addition to the well-known proofreading from this mismatch removal, the premature termination in DNA polymerization, due to the failure of the efficient removal of the mismatched nucleotides, worked as an off-switch in maintaining the high fidelity in DNA replication from exo+ polymerase.

• Journal of the Korea Computer Graphics Society
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• v.27 no.4
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• pp.1-9
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• 2021

Segmenting the compartments of neurons, such as axons, dendrites, and cell bodies, is helpful in the analysis of neurological phenomena. Recently, there have been several studies to segment the compartments through deep learning. However, this approach has the potential to include errors in the results due to noise in data and differences between training data and actual data. Therefore, in order to use these for actual analysis, it is essential to proofread the results. The proofreading process requires a lot of effort and time because an expert must perform it manually. We propose an interactive neuron structure proofreading method that can more easily correct errors in the segmentation results of a deep learning. This method proofread the neuron structure based on the characteristics of the neuron with structural consistency, so that a high-accuracy proofreading result can be obtained with less interaction.

• Journal of The Korean Association of Information Education
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• v.11 no.1
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• pp.107-116
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• 2007

To produce accurate editing results, the ambiguity of editing scopes related to marked correction signs should be solved. Proofreading the web document modifies the document structures, and the modified structures should be robustly valid for the defined DTD. This paper presents a pen-based proof-reading interface in the XML document. In the proposed interface, correction signs are free-drawn, and the editing scopes are recognized and revised based on the contexts of the document to minimize the ambiguity of the editing scopes. The proposed interface provides both implicit and explicit modification methods for document structures. As a result, the editing scopes processed in the proposed interface are more accurate, and the document structures are maintained valid for DTD after the editing.

• Industrial Engineering and Management Systems
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• v.9 no.3
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• pp.275-284
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• 2010

Offshore software development (OSD) is international business collaboration. OSD projects often encounter intercultural and inter-linguistic problems disturbing the projects. Business documents are formal media of information and knowledge for OSD. While OSD documents should convey common understanding of the OSD products, the documents may contain unsuitable expressions which draw misunderstanding of the required products and offensive issues for the collaboration. Intercultural and inter-linguistic differences cause mistakes and inappropriate expressions. OSD from Japan to China is the largest in Asia, and Japanese language is often used in OSD documents. Large similarity is found between Japanese and Chinese in their languages, while many differences exist even for the same word. The similarity induces to write unsuitable expressions for both sides of OSD. To introduce risks for OSD projects caused by unsuitable or inappropriate expressions in OSD documents, we propose to apply a proofreading system of Japanese documents for OSD. Japanese consignor uses the system to refine OSD documents written by Japanese engineers for Chinese readers, and Chinese consignee uses it to refine Japanese documents written by Chinese Engineers as derivatives of OSD projects. Effectiveness of applying the proofreading system is discussed for actual projects.

• Proceedings of the Korean Society for Language and Information Conference
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• 1998.02a
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• pp.328-333
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• 1998