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In vitro cytotoxic evaluation of some essential oils

  • 발행 : 2003.12.30

초록

Seven essential oils were tested for in vitro cytotoxicity against the cancerous cell lines A-549, HEp-2 and DLA and normal BRL-3A, NRK-49F and Vero cell lines using standard MTT, SRB and dye exclusion techniques. The A-549 cell line was found to be the most susceptible to all the essential oils. The essential oils of A. nilagirica, A. calamus and O. sanctum were found to be the more active against these cells with mean $CTC_{50}$ values of 17.75, 19.00 and $24.37\;{\mu}g/ml$, respectively. The essential oil of Acorus calamus was found to be the most potent with low $CTC_{50}$ values against the cancerous and comparatively higher $CTC_{50}$ values against the normal cell lines. Artemisia pellens and Pelargonium graveolens oils also showed potent activity. These oils merit further investigation to identify the active principles and nature of the anti tumor activity in animal models.

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참고문헌

  1. Anonymous. (1995) The Wealth of India, Council of Scientific and Industrial Research, New Delhi.
  2. Aruna K, Sivaramakrishnan VM. (1996) Anticarcinogenic effects of the essential oils from Cumin, Poppy and Basil. Phytother. Res. 10, 577-580. https://doi.org/10.1002/(SICI)1099-1573(199611)10:7<577::AID-PTR906>3.0.CO;2-Q
  3. Badami S, Manohara Reddy SA, Kumar EP, Vijayan P, Suresh B. (2003) Anti tumor activity of total alkaloid fraction of Solanum pseudocapsicum leaves. Phytother. Res. 000 (In press).
  4. Belman S. (1983) Onion and garlic oil inhibit tumor promotion. Carcinogenesis 4, 1063-1065. https://doi.org/10.1093/carcin/4.8.1063
  5. Chen CY, Shen YC, Chen YJ, Sheu JH, Duh CY. (1999) Bioacitve sesquiterpenes from a Taiwanese marine sponge Parahigginsia sp. J. Nat. Prod. 62, 573-576. https://doi.org/10.1021/np980491p
  6. De la Puerta R, Saenz MT, Garcia MD. (1993) Cytostatic activity against Hep-2 cells and antibacterial activity of essential oil from Helichrysum picardii. Phytother. Res. 7, 378-380. https://doi.org/10.1002/ptr.2650070511
  7. Duke JA, Ayensu ES. (1985) Medicinal Plants of China, 14 pp. 52-361. Reference publications, Inc. Algonac, Michigon.
  8. Francis D, Rita L. (1986) Rapid colorimetric assay for cell growth and survival: modifications to the tetrazolium dye procedure giving improved sensitivity and reliability. J. Immunol. Methods 89, 271-277. https://doi.org/10.1016/0022-1759(86)90368-6
  9. Hwang YK, Kim DC, Hwang WH. (1999) Anti tumor effect of Mugwort (Artemisia princes) in vivo. Nat. Prod. Sci. 5, 1-6.
  10. KarthikeyanK, Gunasekaran P, Ramamurthy, Govindasamy S. (1999) Anticancer activity of Ocimum sanctum. Pharm. BioI. 37, 285-290. https://doi.org/10.1076/phbi.37.4.285.5801
  11. Lama S, Santra SC. (1979) Development of Tibetan plant medicine. Sci. Cult. 45, 262-265.
  12. Moldeus P, Hogberg J, Orrhenius S, Fleischer S, Packer L. (1978) Methods in Enzymol, Vol. 52, pp. 60-71, Academic Press, New York. https://doi.org/10.1016/S0076-6879(78)52006-5
  13. Motley TJ. (1994) The Ethnobotony of sweet flag, Acorus calamus (Araceae). Econ. Bot. 48, 397-412. https://doi.org/10.1007/BF02862235
  14. Philip S, Rista S, Dominic S, Anne M, James M, David V, Jonathan TW, Heidi B, Susan K, Michale RB. (1990) New colorimetric cytotoxic assay for anti cancer drug screening. J. Natl. Cancer Inst. 82, 1107-1112. https://doi.org/10.1093/jnci/82.13.1107
  15. Prakash J, Gupta SK, Joshi S. (1999) Antiproliferative studies on Ocimum sanctum. Indian J. Pharmacol. 31, 73-76.
  16. Satyavati GV, Gupta AK, Tandon N. (1987) Medicinal Plants of India, Vol. 2, pp. 354-366. Indian Council of Medical Research, New Delhi.
  17. Setzer WN, Setzer Me, Moriarity DM, Bates RB, Haber WA. (1999) Biological activity of the essential oil of Myrcianthes sp. Nov. "Black fruit" from Monteverde, Costa Rica. Planta Med. 65, 468-469. https://doi.org/10.1055/s-2006-960816
  18. Vijayan P, Vinod kumar S, Dhanaraj S A, Shrishailappa Badami, Suresh B. (2002) In vitro cytotoxicity and anti tumor properties of the total alkaloid fraction of unripe fruits of Solanum pseudocapsicum. Pharm. BioI. 40, 456-460. https://doi.org/10.1076/phbi.40.6.456.8444
  19. Vijayan P, Vinod S Kumar, Dhanraj SA, Pulok K Mukherjee, Suresh B. (2003) In vitro cytotoxicity and antitumor properties of Hypericum Mysorens and Hypericum patulam. Phytother. Res. 000 (In press).
  20. Wang Q, Zhou BN, Zhang RW, Lin YY, Cordell GA. (1996) Cytotoxicity and NMRspectral assignments of ergolide and bigelovin. Planta Med. 62, 166-168. https://doi.org/10.1055/s-2006-957843
  21. Zheng G, Kenney PM, Lam LKT. (1993) Potential anticarcinogenic natural products isolated from lemongrass oil and galanga root oil. Zj. Agric. Food chem. 41, 153-156. https://doi.org/10.1021/jf00026a001