밤나무 꽃과 잎의 화학성분 및 항균활성 검색 -제2보. 항균활성 검색-

Screening of Antimicrobial Activity from Castanea crenata Sieb. et Zucc. Leaves and Flowers. -II. Screening of Antimicrobial Activities-

  • 김해영 (경희대학교 생명과학부 식품공학과) ;
  • 조규성 (한경대학교 이공학부 식품공학과,식품생물산업연구소)
  • Kim, Hae-Yeong (Department of Food Science and Technology, Kyung Hee University) ;
  • Cho, Gyu-Seong (Department of Food Science and Technology, Han Kyong National University)
  • 발행 : 2003.08.30

초록

밤나무의 밤꽃과 밤잎의 항균활성 효과를 조사하기 위하여, 시료를 80% methanol로 추출하여 용매분획을 하였다. 용매분획별 수율은 밤꽃에서 ethylacetate 1.94%, methanol 4.75% 및 water 7.81%였고, 밤잎은 ethylacetate 2.24%, methanol 5.03% 및 water 8.47%였다. 밤꽃과 밤잎의 methanol 분획 추출물은 Gram양성 및 음성의 모든 균주에서 강한 항균활성을 보였으며, ethylacetate 및 water 분획 추출물은 약한 항균활성을 보였다. 최소저해농도는 $60\;{\mu}g/disc$ 이상에서 항균력이 인정되었다. Bacillus. subtilis에 대한 생육저해 효과는 control에 비하여 밤꽂과 밤잎 추출물은 모두 낮은 생육저해 효과를 보였으며, 또한 각 추출물은 모두 배양 15시간까지는 현저히 낮은 생육저해를 보이다가 그 후는 약간 높아졌다. 밤꽃 추출물에서는 methanol 추출물이 가장 높은 생육저해효과를 보인 반면에, water 추출물은 다른 추출물에 비하여 낮은 생육저해를 보였으며, 밤잎 추출물은 각 추출물에서 거의 비슷한 생육저해를 보였다. 밤꽃과 밤잎의 methanol 분획 추출물을 B. subtilis에 각각 500 ppm씩 처리한 후, 주사전자현미경(SEM)으로 관찰한 결과, 추출물을 처리하지 아니한 경우는 전형적인 Bacilli의 형태를 보였지만, methanol 분획추출물을 처리한 경우는 세포의 형태가 일그러지고 손상을 입은 형태를 보였다.

Chestnut (Casranea crenata S. et Z.) leaves and flowers were extracted with 80% methanol and then fractionated with ethylacetate, methanol and water. Their antimicrobial activities in each fraction were investigated. Methanol fraction of the chestnut leaves and flower showed strong antimicrbial activities against both of Gram positive and Gram negative bacteria. The ethylacetate and water fraction, however, showed only weak antimicrobial activities when the antimicrobial activities were occurred. Minimal inhibitory concentration (MIC) of the methanol extracts of the chestnut leaves and flowers against 5 strains of Gram positive and Gram negative bacteria were at $60\;{\mu}g/disc$. The extracts of the chestnut leaves and flowers inhibited the growth of Bacillus subtilis at 0.5% (w/w) concentration. In order to investigate the effect of extraction methods on the B. subtilis, scanning electron microscope was used. The B. subtilis was damaged when the methanol extracts of the chestnut leves and flowers were at 500 ppm.

키워드

참고문헌

  1. Kim, J. H., Kim, J. C., Ko, K. C., Kim, G. R. and Lee, J. C. (1998) In Detailed expositions of a fruit tree and horticulture (4th ed.), Hyangmoonsa, Seoul, pp. 382-396
  2. Suh, K. B., Han, P. J. and Lee, S. J. (1974) Studies on the processing of chestnut (Castanea pubinervis Sckneid). Korean J. Food Sci. Technol. 6, 98-108
  3. Kim, S. K., Jeon, Y. J., Kim, Y. T., Lee, B. J. and Kang, O. J. (1995) Physicochemical and textural properties of chestnut starches. J. Korean Soc. Food Nutr. 24, 594-600
  4. Yim, H., Kim, C. O., Shin, D. W. and Suh, K. B. (1980) Study on the storage of chestnut. Korean J. Food Sci. TechnoI. 12, 170-175
  5. Nha, Y. A. and Yang, C. B. (1996) Changes of constituent components in chestnut during storage. Korean J. Food Sci. TechnoI. 28, 1164-1170
  6. Kim, S. K., Jeon, Y. J., Kim, Y. T., Lee, B. J. and Kang, O. J. (1995) Sensory evaluation and retrogradation properties of chestnut mook. J. Korean Soc. Food Nutr. 24, 601-605
  7. Choi, O. B. (1999) Physiological activity and chemical composition of Castanea crenata leaf tea, Ph.D. Thesis, Chonnam University, Kwangju, Korea
  8. Lee, Y. S. (1996) Chenaical components and biological activities of chestnut flower (Castanea crenata). Ph.D. Thesis, Kyongsang University, Jinju, Korea
  9. Kwon, E. J., Kim, Y. C., Kwon, M. S., Kim, C. S., Kang, W. W., Lee, J. B. and Chung, S. K. (2001) Antioxidative activity of solvent fraction and isolation of andoxidative compound from chestnut husk. J. Korean Soc. Food Sci. Nutr. 30, 726-731
  10. Valencia-Barrera, R.M., Fombella-Blanco, M.A., Femandez-Gonzalez, D., Diaz-Gonzales, T. E., (1994) Pollen spectra of honey from different phytogeographical regions of the Leon proven: Dep. de Biologia vegetal Universidad de Leon, E-2471 Leon, Spain Grrana, 33, 268-275
  11. Bunchbauer, Gerhard, Jirovetz, Leopoid, Wasicky, Michael, Nikiforov, Alexej. (1994) Volotile common horsechestnut blossoms were analyzed by GC/FID, GC/FTIR/MSD and GC sniffng technique. J. Essent. OiI Res. 6, 93-95 https://doi.org/10.1080/10412905.1994.9698335
  12. Yamaguchi, K., Shibamoto, T. (1980) Volatile constituents of the chestnut flower (Castanea crenata). J. Asric. Food Chem. 28, 82-84 https://doi.org/10.1021/jf60227a021
  13. Amito M. J., Aubert S. Gonnet M. Tachmi M. (1989) The phenolic compounds in honeys preliminary study on identification and family quantfication Lab. De biochanie metabolique et technologie station de technologie des products vegetaux. Apidologie 20, 115-126 https://doi.org/10.1051/apido:19890202
  14. Talpay B. (1988) The ingredients of honey citric acid (citrate). Dtsch. Lebensm-Rmdsch 84, 41-44
  15. Battaflini, M. B. (1974) Sugar composition of some uniflora honeys and the nectrars they are derived from. Apic. Abstn. 25, 123-129
  16. Kullmann E. (1974) Qulitative determination of free amino acids and the honey dew honeys mixed honey and honeys from flowers. Apidologie 5, 21-38 https://doi.org/10.1051/apido:19740102
  17. Park, C. S., Kim, W. S., Ahn, C. Y. and Lee, M. H. (1999) In Chestnut, Persimmon, date, walnut. Naewyoi Press, Seoul. pp. 75-94
  18. Kwan, S. B. (1990) In Atlas of pharmacognogy encyclophedia (Ptants). Yonglim Press, Seoul, pp. 806-808
  19. Lee, T .B. (1994) In Illustrated fIora of Korea. Hyangmoon Press, Seoul. p. 274
  20. Roberto Chiej (1984) In The MacDonaId encylopedia of medical plants. MacDonald Press, Britain. p. 72
  21. Korean national institute of health. (1996) Antimicrobial test. pp. 59-70
  22. Baek, J. W., Chung, S. H. and Moon, G. S. (2002) Antimicrobial activities of ethanol extracts from Korean bamboo culms and leaves. Korean J. Food Sci. Technol. 34, 1073-1078
  23. Oh, D. H., Lee, M. K. and Park, B. K. (1999) Antimicrobial activities of commercially available tea on the harmful foodborne organisms. J. Korean Soc. Food Sci. Nutr. 28, 100-106
  24. Yeo, S. G., Ahn, C. W., Kim, I. S., Park, Y. H. and Kim, S. B. (1995) Antimicrobial effect of tea extract from green tea, oolong tea and black tea. J. Korean Soc. Food. Nutr. 24, 293-298
  25. Ikigai, H., Nakae, T., Hara, Y. and Shimamura, T. (1993) Bactericidal catechins damage the lipid bilayer. Biochem. Biophy. Acta 1147, 132 https://doi.org/10.1016/0005-2736(93)90323-R
  26. Kong, Y. J., Kang, T. S., Lee, M. K., Park, B. K. and Oh, D. H. (2001) Antimicrobial and antioxidadve activities of solvent fractions of Quercus monsolica leaf. J. Korean Soc. Food Sci. Nutr. 30, 338-343
  27. EL-Shenawy, M. A. and Marth, E. H. (1988) Inhibition and inactivation of Listeria monocytogenes by sorbic acid. J. Food Port. 51, 525-528 https://doi.org/10.4315/0362-028X-51.7.525
  28. EL-Shenawy, M. A. and Marth, E. H. (1989) Inactivation of Listeria monocytogenes by sodium benzonate together with some organic acids. J. Food Port. 52, 771-775 https://doi.org/10.4315/0362-028X-52.11.771
  29. Chung, K. H., Lee, S. H., Lee, Y. C. and Kim, J. T. (2001) Antimicrobial activity of Omija (Schizandra chinensis) extracts. J. Korean Soc. Food Sci. Nutr. 30, 127-132
  30. Preseott, L. M., Harley, J. P. and Klein, D. A. (2002) In Microbiology (5th ed), Antimicrobial chemotherapy. McGraw-Hill Co., N.Y, USA, pp. 805-822
  31. Cho, S. H., Lee, H. C., Seo, I. W., Kim, Z. U., Chang, Y. S. and Shin, Z. I. (1991) Efficacy of grapefruit seed extract in the preservation of Satsuma mandarin. Korean J. Food Sci. Technol. 23, 614-618