Journal of the Korean Chemical Society (대한화학회지)

Korean Chemical Society (대한화학회)
권호 표지
DOI QR코드

DOI QR Code

Activation of Cabbage Phospholipase D by Polyamines

폴리아민에 의한 양배추 포스포리파제 D의 활성화

  • Eun-Hie Koh (Department of Chemistry, Duksung Wommen's University)
  • 고은희 (덕성여자대학교 화학과)
  • Published : 2003.10.20
Download PDF
⟨ Previous Next ⟩

Abstract

The effect of polyamines on the cabbage phospholipase D(PLD) activity was investigated. The PLD activity was determined by pH-stat titration of phosphatidic acid, one of the enzymatic reaction product, using phosphatidyl choline small unilamellar vesicles as a substrate. The cabbage PLD was activated approximately 4 fold by spermine at 1 mM concentration. This spermine effect appears to be similar to the previous report on the PLD activation of rat brain mitochondrial fraction. It was also found that cationic polypetides such as polylysine and polyhistidine exerted a marked enhancement effect on the cabbage PLD. Particularly polyhistidine exerted approximately 5.5 fold enhancement effect at 0.062 mM concentration. The polyamine effect on the cabbage PLD was reexamined in the phosphatidylcholine/sodium dodecyl sulfate mixed micellar system. The relevance of polyamine effect on PLD activity is discussed in relation to the active site of PLD.

양배추 포스포리파제 D(PLD)에 대한 포리아민들의 영향을 조사하였다. PLD 활성도는 phosphatidylcholine small unilamellar 베시클을 기질로 하여 pH- stat 방법으로 생성물질 phosphatidic acid를 적정하여 결정하였다. 양배추 PLD는 스퍼민 1 mM 농도에서 약 4배 활성화되었다. 이 스퍼민 효과는 전에 보고된 쥐 뇌 미토콘드리아 분획의 PLD 활성과 유사한 결과를 보여주고 있다. 양이온성 polypeptide인 polylysine과 polyhistidine에 의해서도 양배추 PLD가 상당히 활성화되는 것을 알았다. 특히 polyhistidine은 0.062 mM 농도에서 약 5.5배의 활성화 효과를 나타냈다. 이 포리아민 효과는 phosphatidylcholine/sodium dodecyl sulfate 혼합미셀계에서도 재확인하였다. 포리아민에 대한 PLD 활성화의 의미를 PLD 활성부위와 관계 지워 고찰하였다.

Keywords

References

  1. Heller, M. Adv. Lipid Res. 1978, 16, 267. https://doi.org/10.1016/B978-0-12-024916-9.50011-1
  2. Wang, X; Xu, L; Zheng, L. J. Biol. Chem. 1994, 269, 20312.
  3. Kim, D.-U.; Roh, T.-Y.; Lee, J.; Noh, J.-Y.; Jang, Y.-J.; Hoe, K.-L.; Yoo, H.-S.; Choi, M.-U. Biochim. Biophys. Acta 1999, 1437, 409. https://doi.org/10.1016/S1388-1981(99)00020-7
  4. Munnik, T.; Irvine, R. F.; Musgrave, A. Biochim. Biophys. Acta 1998, 1389, 222. https://doi.org/10.1016/S0005-2760(97)00158-6
  5. Kent, C. Annu. Rev. Biochem. 1995, 64, 315. https://doi.org/10.1146/annurev.bi.64.070195.001531
  6. Fan, L.; Zheng, S.; Wang, X. Plant Cell, 1997, 9, 2183. https://doi.org/10.1105/tpc.9.12.2183
  7. Lee, S.; Suh, S.; Kim, S.; Crain, R. C.; Kwak, J. M.; Nam, H. G.; Lee, Y. Plant J. 1997, 12, 547. https://doi.org/10.1046/j.1365-313X.1997.00547.x
  8. Zien, C. A.; Wang, C.; Wang, X.; Welti, R. Biochim. Biophys. Acta 2001, 1530, 236. https://doi.org/10.1016/S1388-1981(01)00091-9
  9. Young, S. A.; Wang, X.; Leach, J. E. Plant Cell, 1996, 8, 1079. https://doi.org/10.1105/tpc.8.6.1079
  10. Voisine, R.; Vezina, L.; Willemot, C. Plant Physiol. 1993, 102, 213. https://doi.org/10.1104/pp.102.1.213
  11. Ryu, S. B.; Karlsson, B. H.; Ozgen, M.; Palta, J. P. Proc. Natl Acad. Sci. U.S.A. 1997, 94, 12717. https://doi.org/10.1073/pnas.94.23.12717
  12. Dittrich, N.; Haftendorn, R.; Ulbrich-Hofmann, R. Biochim. Biophys. Acta. 1998, 1391, 265. https://doi.org/10.1016/S0005-2760(97)00208-7
  13. Teti, D.; Visalli, M; McNair, H. J. Chromatography B 2002, 781, 107. https://doi.org/10.1016/S1570-0232(02)00669-4
  14. Coburn, R. F.; Jones, D. H.; Morgan, C. P.; Baron, C. B.; Cockcroft, S. Biochim. Biophys. Acta 2002, 1584, 20. https://doi.org/10.1016/S1388-1981(02)00265-2
  15. Koh, E.-H. J. Korean Chem. Soc. 2000, 44, 448.
  16. Lee, J.; Choi, M.-U. Bull. Korean Chem. Soc. 1996, 17, 905.
  17. Lee, C. H.; Choi, M.-U. Bull. Korean Chem. Soc. 1984, 5, 154. https://doi.org/10.1007/BF02697670
  18. Vepa, S.; Scribner, W. M.; Natarajan, V. Am. J. Physiol. 1997, 27, 2608.
  19. Bashford, C. L.; Alder, G. M.; Graham, J. M.; Menestrina, G.; Pasternak, C. A. J. Menbrane Biol. 1988, 103, 79. https://doi.org/10.1007/BF01871934
  20. Eichberg, J.; Zetisky, W.; Bell, M. E.; Cavanagh, E. J. Neurochem, 1981, 36, 1868. https://doi.org/10.1111/j.1471-4159.1981.tb00444.x
  21. Haber, M. T.; Fukui, T.; Lebowitz, M. S.; Lowenstein, J. M. Arch. Biochem. Biophys. 1991, 288, 243. https://doi.org/10.1016/0003-9861(91)90191-K
  22. Tassani, V.; Bibom, C.; Toniello, A.; Siliprands, D. Bichim. Biophys. Acta. 1995, 1348, 265.
  23. Xie, L. H.; John, S. A.; Weiss, J. N. J. Gen. Physiol. 2002, 120, 53. https://doi.org/10.1085/jgp.20028576
  24. Leiros, I.; Secundo, F.; Zambonelli, C.; Servi, S.; Hough, E. Structure, 2000, 8, 655. https://doi.org/10.1016/S0969-2126(00)00150-7

Cited by

  1. 양배추 포스포리파제 D 활성에 미치는 포스포리파제 A2의 영향 vol.52, pp.5, 2003, https://doi.org/10.5012/jkcs.2008.52.5.488