Fisheries and Aquatic Sciences

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Algicidal Activity of Substance Purified from Marine Bacteria Metabolites against Cochlodinium polykrikoides

  • Byun Hee-Guk (Department of Chemistry, Pukyong National University) ;
  • Jeong Seong-Youn (Department of Microbiology, Pukyong National University) ;
  • Park Young-Tae (South Sea Fisheries Research Institute, National Fisheries Research and Development Institute) ;
  • Lee Won-Jae (Department of Microbiology, Pukyong National University) ;
  • Kim Se-Kwon (Department of Chemistry, Pukyong National University)
  • Published : 2002.09.01
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Abstract

Marine bacteria producing algicidal substance against Cochlodinium polykrikoides was screened and isolated from seawater. Metabolite of marine bacteria Micrococcus sp. LG-5 showed strong algicidal activity against C. polykrikoides. C. polykrikoides was inhibited above $90\%$ at $5\%$ solution of the metabolite within 24 hrs. Algicidal substance from the metabolite was extracted with ethyl acetate, and then purified by PTLC and reversed-phase HPLC. Algicidal activity of purified compound against C. polykrikoides was above $90\%$ at 3.7, 11.0 and 33.0${\mu}g/mL$ concentration after 12, 9 and 3 hrs, respectively. Ninety percent inhibition of other red tides, Gymnodinium sanguineum and Gyrodinium impudicum was observed when treated with 3.7${\mu}g/mL$ of purified compound within a period of 12 hrs. The microscopic view of red tides treated with purified compound showed the deformations such as cell node cuts and swelling of cells.

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References

  1. Cho, E.S., C.S. Kim, S.G. Lee and Y.K. Chung. 1999. Binding of alcian blue applied to harmful microalgae from Korean coastal waters. Bull. Natl. Fish. Res. Dev. Inst., 55, 133-138
  2. Dakhama, A., J. de la Noue and M.C. Lavoie. 1993. Isolation and identification of antialgal substance produced by Pseudomonas aemginosa. J. Appl. Phytoplank., 5, 297-306
  3. Droop, M.R 1967. A procedure for routine purification of algal cultures with antibiotics. Brit. Phycol. Bull., 3, 295-297 https://doi.org/10.1080/00071616700650171
  4. Fukami, K., .A. Yuzawa, T. Nishijima and Y. Hata. 1992. Isolation and properties of a bacterium inhibiting the growth of Gymnodinium nagasakiense. Nippon Suisan Gakkaishi, 58, 1073-1077 https://doi.org/10.2331/suisan.58.1073
  5. Guillard, R.R.L. and J.H. Ryther. 1962. Studies of marine planktoric diatoms 1. Cyclotella nana HUSTEDT and Detonua confervacea (CLEVE) GRAN. Can. J. Microbiol., 229-239
  6. Imai, I., S. Nagagiri, K. Nagai, K. Nagasaki, S. Itakura and M. Yamaguchi. 1998. Fluctuations of algicidal microorganisms against the harmful dinoflagellate Heterocapsa circularisquama in Ago Bay, Mie prefecture. Japan. Bull. Nansei Natl. Fish. Res. Inst., 31, 53-61 (in Japanese)
  7. Jeong, S.Y., Y.T. Park and WJ. Lee. 2000. Isolation of marine bacteria killing red tide microalgae. J. Korean Fish. Soc., 33, 331-338 (in Korean)
  8. Kim, C.S., S.G. Lee and H.G. Kim. 2000b. Biochemical responses of fish exposed to a harmful dinoflagellate Cochlodinium -polykrikoides. J. Experimental Marine Biology and Ecology, 254, 131-141 https://doi.org/10.1016/S0022-0981(00)00263-X
  9. Kim, C.S., H.M. Bae, S.J. Yun, Y.C. Cho and H.G. Kim. 2000 c. Ichthyotoxicity of a harmful dinoflagellate Cochlodinium polykrikoides: Aspect of hematological responses of fish exposed to algal blooms. J. Fish. Sci. Tech., 3, 111-117
  10. Kim, H.G., Y.C. Cho, C.S. Jeong, H.M. Bae, B.C. Oh, C.K. Lee, E.S. Cho, C.S. Kim, W.A. Lim, S.H. Youn and J.B. Kim. 2000a. In Harmful Algal Blooms in Korean Coastal Waters in 1999, National Fisheries Research & Development Institute Republic of Korea, Hangul Graphics Co., Busan, Korea (in Korean)
  11. Kim, M.C., I. Yoshinaga, I.Imai, K. Nagasaki, S. Itakura and Y. Ishida. 1998. A close relationship between algicidal bacteria and termination of Heterosigma akashiwo (Raphidophyceae) bloom in Hiroshima Bay, Japan. Mar. Ecol. Prog. Ser., 170, 25-32 https://doi.org/10.3354/meps170025
  12. Lee, J.S. 1996. Bioactive components from red tide plankton, Cochlodinium polykrikoides. J. Kor. Fish. Soc., 29, 165-173 (in Korean)
  13. Mitsutani, A., K. Takasue, M. Kirita and Y. Ishida. 1992. Lysis of Skeletonema costatum by Cytophaga sp. isolated from the costal water of the Ariake Sea. Nippon Suisan Gakkaishi, 58, 2159-2169 https://doi.org/10.2331/suisan.58.2159
  14. Nakanishi, K., I. Karube, S. Hiroshi, A. Uchida and Y. Ishida. 1996. Detection of the red tide-causing plankton Chattonella marina using a piezoelectric immunosensor. Analytical Chimica Acta, 325, 73-80 https://doi.org/10.1016/0003-2670(96)00006-2
  15. Onoue, Y. and K. Nozawa. 1989. Separation of toxins from harmful red tides occurring along the coast of Kagoshima prefecture, In Red Tides: Biology, Environmental Science, and Toxicology, T. Okaichi, D.M. Anderson and T. Nemoto, eds. Elsevier, New York, pp. 371-374
  16. Taga, N. 1968. Some ecological aspects of marine bacteria in the Kuroshio current. Bull. Misaki Mar. Biol. Kyoto Univ., 12, 50-76
  17. Uchida, T., S. Toda, Y. Matsuyama, M. Yamaguchi, Y. Kotani and T. Honjo. 1999. Interactions between the red tide dinoflagellates Heterocapsa circularisquama and Gymnodinium mikimotoi in laboratory culture. J. Experimental Marine Biology and Ecology, 241, 285-299 https://doi.org/10.1016/S0022-0981(99)00088-X
  18. Yanagi, T., T. Yamamoto, Y. Koizumi, T. Ikeda, M. Kamizono and H. Tamori. 1995. A numerical simulation of red tide formation. J. Marine Systems, 6, 269-285 https://doi.org/10.1016/0924-7963(94)00027-9
  19. Yoshinaga, I., T. Kawai, T. Takeuchi and Y. Ishida. 1995. Distribution and fluctuation of bacteria inhibiting the growth of a marine red tide phytoplankton Gymnodinium mikimotoi in Tanabe Bay (Wakayama Pref., Japan). Fisheries Sci., 61, 780-786 https://doi.org/10.2331/fishsci.61.780

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  2. Isolation, identification, and algicidal activity of marine bacteria against Cochlodinium polykrikoides vol.20, pp.6, 2002, https://doi.org/10.1007/s10811-008-9312-x
  3. Isolation of marine algicidal bacteria from surface seawater and sediment samples associated with harmful algal blooms in Korea vol.52, pp.1, 2002, https://doi.org/10.7845/kjm.2016.5048