Journal of Periodontal and Implant Science

Korean Academy of Periodontology (대한치주과학회)
권호 표지

The effect of PDGF-BB loaded TCP/chitosan microgranules on new bone formation

혈소판유래성장인자를 함유한 TCP-chitosan 미세과립이 신생골조직 형성에 미치는 영향에 관한 연구

  • Seol, Yang-Jo (Department of Periodontology, College of Dentistry, Seoul National University, Department of Periodontics, Samsung Medical Center, Sungkyunkwan University School of Medicine) ;
  • Lee, Jue-Yeon (Department of Pharmacy, College of Pharmacy, Ewha Womans University) ;
  • Kye, Seung-Beom (Department of Periodontics, Samsung Medical Center, Sungkyunkwan University School of Medicine) ;
  • Lee, Young-Kyu (Department of Periodontics, Samsung Medical Center, Sungkyunkwan University School of Medicine) ;
  • Kim, Won-Kyeong (Department of Periodontology, College of Dentistry, Seoul National University, Department of Dentistry, Asan Medical Center) ;
  • Lee, Yong-Moo (Department of Periodontology, College of Dentistry, Seoul National University) ;
  • Ku, Young (Department of Periodontology, College of Dentistry, Seoul National University) ;
  • Han, Soo-Boo (Department of Periodontology, College of Dentistry, Seoul National University) ;
  • Lee, Seung-Jin (Department of Pharmacy, College of Pharmacy, Ewha Womans University) ;
  • Chung, Chong-Pyoung (Department of Periodontology, College of Dentistry, Seoul National University) ;
  • Rhyu, In-Chul (Department of Periodontology, College of Dentistry, Seoul National University)
  • 설양조 (서울대학교 치과대학 치주과학교실, 성균관대학교 의과대학 삼성의료원 치과진료부 치주과) ;
  • 이주연 (이화여자대학교 약학대학 약학과) ;
  • 계승범 (성균관대학교 의과대학 삼성의료원 치과진료부 치주과) ;
  • 이영규 (성균관대학교 의과대학 삼성의료원 치과진료부 치주과) ;
  • 김원경 (서울대학교 치과대학 치주과학교실, 서울아산병원 치과) ;
  • 이용무 (서울대학교 치과대학 치주과학교실) ;
  • 구영 (서울대학교 치과대학 치주과학교실) ;
  • 한수부 (서울대학교 치과대학 치주과학교실) ;
  • 이승진 (이화여자대학교 약학대학 약학과) ;
  • 정종평 (서울대학교 치과대학 치주과학교실) ;
  • 류인철 (서울대학교 치과대학 치주과학교실)
  • Published : 2002.09.30
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Abstract

The purpose of this study was to evaluate newly fabricated tricalcium phosphate(TCP)/chitosan microgranuls as bone substitutes. TCP/chitosan microgranules were fabricated by dropping TCP-chitosan suspension into the NaOH/ethanol solution. The size of microgranules could be controllable via airflow rate. PDGF-BB was loaded into the fabricated granules via freeze-drying methods(300 ng/20 mg). To evaluate cell proliferation, cultured osteoblasts cell lines(MC3T3-El) was dropped on the BioOss(R), chitosan microgranules, TCP/chitosan microgranules and cultured for 1, 7 , 14, and 28 days. Scanning electron microscopic observation was done after 7 days of culture and light microscopic examination was done after 28 days of culture. PDGF-BB release from the microgranules was tested. Rabbit calvarial defects(8 mm in diameter) were formed and chitosan, TCP/chitosan, PDGF-TCP/chitosan microgranules, and BioGran(R) were grafted to test the ability of new bone formation. At SEM view, the size of prepared microgranules was 250-1000 um and TCP powders were observed at the surface of TCP/chitosan microgranules. TCP powders gave roughness to the granules and this might help the attachment of osteoblasts. The pores formed between microgranules might be able to allow new bone ingrowth and vascularization. There were no significant differences in cell number among BioOss(R) and two microgranules at 28 day. Light and scanning electron microscopic examination showed that seeded osteoblastic cells were well attached to TCP/chitosan microgranules and proliferated in a multi-layer. PDGF-BB released from TCP/chitosan microgranules was at therapeutic concentration for at least 1 week. In rabbit calvarial defect models, PDGF-TCP/chitosan microgranules grafted sites showed thicker bone trabeculae pattern and faster bone maturation than others. These results suggested that the TCP/chitosan microgranules showed the potential as bone substitutes.

Keywords

References

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