ALGAE

The Korean Society of Phycology (한국조류학회(藻類))
권호 표지
DOI QR코드

DOI QR Code

Phylogenetic Relationships between Ulva conglobata and U. pertusa from Jeju Island Inferred from nrDNA ITS 2 Sequences

  • Kang, Sae-Hoon (Faculty of Applied Marine Sciences, Cheju National University) ;
  • Lee, Ki-Wan (Faculty of Applied Marine Sciences, Cheju National University)
  • Published : 2002.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

In this study the length of ITS2 from four species of the Ulvaceae in Jeju Island varied between 167 and 203 bp. The resuits of this investigation showed that two genus, Ulva and Enteromorpha are grouped in a monophyletic assemblage with 100% bootstrap support in all phylogenetic trees. However, a thorough eamination of these characters from representatives does not provided a way to identify any unique morphological features of clasdes in this tree. This study reveals that Ulva conglobata and Ulva pertusa belong to one clade in the phylogenetic tree with the samples from Jeju Island, Korea.

Keywords

References

  1. Bae E.H. and Lee I.K. 2001. Umbraulva, a new genus based on Ulva japonica (Holemes) Pepenfuss (Uvaceae, Chlorophyta). Algae16: 217-231.
  2. Bakker F.T., Olsen J.L. and van den Hoek C. 1992. Nuclear ribo-somal DNA internal transcribed spacer regions (ITS1 and ITS2) define discrete biogeographic groups in Cladophora albida(Chlorophyta). J. Phyeol. 28: 839-845. https://doi.org/10.1111/j.0022-3646.1992.00839.x
  3. Baldwin C.C. and Johnson G.D. 1993. Phylogency of the Epinephelinae (telestei: Serranidae). Bull. Mar. Sci. 52: 240-283.
  4. Blomster J., Maggs C.A. and Stanhope M.J. 1998. Molecular and morphological analysis od Enteromorpha intestinalis and E. compressa (Chlorophyta) in the British isles. J. Phyeol. 34: 319-340. https://doi.org/10.1046/j.1529-8817.1998.340319.x
  5. Blomster J., Maggs C.A. and Stanhope M.J. 1999. Extensive intraspecific morphological variation in Enteromrpha mus-coides (Chlorophyta) revealed by molecular analysis. J. Phycol. 35: 575-586. https://doi.org/10.1046/j.1529-8817.1999.3530575.x
  6. Blomster J., Hoey E.M., Maggs C.A. and Stanhope M.J. 2000.Species-specific oligonucleotide probes for macroalgae:molecular discrimination of two marine fouling species of Enteromopha(Ulvophyceae). Mol. EeoI. 9: 177-186 https://doi.org/10.1046/j.1365-294x.2000.00850.x
  7. Bonneau R.H., Brehm M.A. and KernA.M. 1997. The impact of psychological stress on the efficacy of anti-vial adoptive immunotherapy in an immunocompromised host. J. NeuroimmunoI. 78: 19-33. https://doi.org/10.1016/S0165-5728(97)00079-9
  8. Callow M.E. 1986. Fouling algae from 'in-service' ships. Bot. Mar. 24: 351-357
  9. Coat G., Dion P., Noailles M.-C., Reviers B.De., Fontaine J.-M.,Ulva armor-icana (Ulcales, Chlorophyta) from the coasts of Brittany(France).II. Nuclear rDNA ITS sequence analysis. Eur. J. Phycol. 33: 81-86. https://doi.org/10.1080/09670269810001736563
  10. Coleman A.W. and Mai J.C. 1997. Ribosomol DNA ITS-1 and ITS-2 sequence comparisons as a tool for predicting genetic relatedness. J. Mol. Evol. 45: 168-177. https://doi.org/10.1007/PL00006217
  11. Fama P., Olsen J.L., Stam T.W. and Procaccini G. 2000. High levels of intra-and iner-individual polymorphism in the rDNA ITS1 of caulerpa racemosa(Chlorophyta) Eur. J. Phycol. 35: 349-356. https://doi.org/10.1080/09670260010001735951
  12. Felsenstein J. 1985. Confidence limits on phylogenies: and approach using the bootstrap. Evolution 39: 783-91. https://doi.org/10.2307/2408678
  13. Fitch W.M. 1971. Toward defining the course of evolution:Minimum change for a specific tree topology. Syst. Zool. 20: 406-416. https://doi.org/10.2307/2412116
  14. Gayral P. 1967. Mise au point sur les Ulvacees (Chlorophycees), particulierment sur les resultants de leur etude en labora-toire. Botaniste 50: 205-251.
  15. Jukes T.H. and Cantor C.R., 1969. Evolution of protein molecules.In: Munro H.N. (ed.), Mammalian Protein Metabolism. Academic Press, New York. pp. 21-132.
  16. Kang G.Y. 2001. Phylogenetic Relationships among Groupers (Genus Epinephelus) Based on Mitochondrial Cytochrome b DNA Sequence. MS thesis, Cheju Nat. Univ.
  17. Kimura M. 1980. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J. Mol. Evol. 16: 111-120. https://doi.org/10.1007/BF01731581
  18. Kumar S., Tamura K., Jakobsen I.B. and Nei M. 2001 MEGA2:Molecular evolutionary genetics analysis software. Bioinformatics 17: 1244-1245. https://doi.org/10.1093/bioinformatics/17.12.1244
  19. Lee I.K. and Kang J.W. 1986. A check List of Marine Algae in Korea. Kor. J. Phycol. 1: 311-325.
  20. Lee I.K., Lee Y.P. and Ahn Y.S. 1986. Flora of Marince Algae in Cheju Island 1. Ulvaceae. Kor. J. Phycol. 1: 157-167.
  21. Leskinen E. and Pamilo P. 1997. Evolution of the ITS sequences of ribomal DNA in Enteromorpha (Chlorophyceae). Hereditas 126: 17-23. https://doi.org/10.1111/j.1601-5223.1997.00017.x
  22. Lobban C.S. and Harrison P.J. 1994. Seaweed ecology and physiology.2nd ed. Cambridge University Press, Cambridge. 366pp.
  23. Maggs C.A. and Ward B.A. 1996. The genus Pikea (Dumontiaceae, Rhodophyta) in England and the North pacific: comparative morpholofical, life history, and mole-cular studies. J. Phycol. 32: 176-193. https://doi.org/10.1111/j.0022-3646.1996.00176.x
  24. Malta E.-J., Draisma S.G.A. and Kamermans P. 1999. FFree-floating Ulva in the southwest Netherlands: species or morpho-types? A morphological, molecualr and ecological comparison. Eur.J. Phycol. 34: 443-454. https://doi.org/10.1080/09541449910001718801
  25. Nakanishi K., Nishijimal M., Nishumura M., Kuwano K. and Saga N. (1996). Bacteria that induce morphogenesis in Ulva pretusa(Chlorophyta) grown under axenic conditions. J. Phycol. 32: 479-482. https://doi.org/10.1111/j.0022-3646.1996.00479.x
  26. Phillips J.A. 1984. The validity of morphological and anatomi-cal characters in distinguishing species of Ulva in southern Australia. pp. 353-361. In: Irvine D.E.G. and John D.M. (eds), Systematics of Green Algae. Academic Press, London. 449pp.
  27. Pillman A., Woolcott G.W., Olsen J.L., Starn W.T. and King R.J. 1997. Inter- and intraspecific genetic variation in Caulerpa(Chlorophyta)based on nuclear rDNA sequences. Eur. J.Phycol. 32:379-86. https://doi.org/10.1080/09670269710001737319
  28. Provasoli L. and Pintner I.J. 1980. Bacteria induced polymor-phism in an axenic laboratory strain of Ulva lactuca (chlorophtceae). J. Phycol. 16: 196-201. https://doi.org/10.1111/j.1529-8817.1980.tb03019.x
  29. Rousseau F., Reviers B.de., Leclerc M.-C, Asensi A. and Delepine R. 2000. Adenocystaceae fam. nov. (Phaeophyceae) based on morphological and molecular evidence. Eur. J. Phycol. 35: 35-43. https://doi.org/10.1080/09670260010001735601
  30. Saitou N. and Nei M. 1987. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol. BioI. Evol. 4: 406-425.
  31. Stiger V., Horiguchi T., Yoshida T., Coleman A.W. and Masuda M. 2000. Phylogenetic relationships of Sargassum(Fargassaceae, Phaeophyceae)with reference to a taxonom-ic revision of the section Phyllocystae based on ITS-2 nr DNA sequences. Phycol. Res.48: 251-260. https://doi.org/10.1111/j.1440-1835.2000.tb00221.x
  32. Swofford D.L. 1998.PAUP:phylohenetic analysis using parsimony. Version 4. Sunderland, MA.
  33. Tamura K. and Nei M. 1993. Estimation of the number of nucleotide substitutions in the control region of mitocheon-drial DNA in humans and chimpanzees. Mol. Biol. Evol. 10: 512-526.
  34. Tan I.H. and Sluiman H.J. 2000. Molecular Phyogeny of selected Ulvaleds(Ulvophyceae, Chlorophyta). http://www.ncbi.nlm.nih.gov / entrez/.
  35. Tan I.H., Blomster J., Hansen G., Leskinen E., Maggs C.A., Mann D.G., Sluiman H.J. and Stanhope M.J. 1999. Molecular phylogenetic evidence for a recersible morpho-genetic switch controlling the gross morphology of two common genera of green seaweeds, Ulva and Enteromorpha. Mol. BioI. Evol. 16: 1011-1018. https://doi.org/10.1093/oxfordjournals.molbev.a026190
  36. Woolcott G.W., lima M. and King R.J. 2000. Speciation within Blidingia minima(Chlorophyta) in Japan: evidence from morphology, ontogency, and analyses of nuclear rDNA ITS sequence. J. phycol. 36: 227-236. https://doi.org/10.1046/j.1529-8817.2000.99034.x

Cited by

  1. Phylogeny of Oedogoniales, Chaetophorales and Chaetopeltidales (Chlorophyceae): inferences from sequence-structure analysis of ITS2 vol.109, pp.1, 2012, https://doi.org/10.1093/aob/mcr275
  2. Systematics of green algae resemblingUlva conglobata, with a description ofUlva adhaerenssp. nov. (Ulvales, Ulvophyceae) vol.50, pp.1, 2015, https://doi.org/10.1080/09670262.2014.994189