Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Protective Effect of Puerariae radix Against Ethanol-induced Apoptosis on Human Neuroblastoma Cell Line SK-N-MC

  • Koo Gyo Sung (Oriental Medicine Research Institute, Kyungwon University) ;
  • Cho Son Hae (Oriental Medicine Research Institute, Kyungwon University) ;
  • Jang Mi Hyean (Department of Physiology. College of Medicine, Kyunghee University) ;
  • Kim Chang Ju (Department of Physiology. College of Medicine, Kyunghee University) ;
  • Kim Ee Hwa (Department of Meridinology, College of Oriental. Medicine, Semyung University) ;
  • Lee Choong Yeol (Oriental Medicine Research Institute, Kyungwon University)
  • Published : 2002.06.01
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Abstract

To investigate whether Puerariae radix (PR) possesses protective effect against ethanol (EtOH)-inducecl apoptosis in the central nervous system, MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay, flow cytometric analysis, DNA fragmentation assay. and reverse transcription-polymerase chain reaction (RT-PCR) were performed on human neuroblastoma cell line SK-N-MC. Morphological and biochemical analyses demonstrated that SK-N-MC cells treated with EtOH exhibit classical apoptotic features. On the other hand, cells pre-treated with PR prior to EtOH exposure showed decreased occurrence of classical apoptotic features. In addition, it was shown that PR pre-treatment inhibits EtOH-induced increases in the levels of mRNA expression of bax and caspase-3, while it further enhances the level of bcl-2 expression. These results suggest that PR may exert protective effects against EtOH-induced apoptosis in human neuroblastoma cells.

Keywords

References

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