Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Neuroprotective Effect of Citri Pericarpium On Transient Global Ischemia in Gerbils

  • Kim Jiae (Department of Neuroscience. Graduate School of East-West Medical Science, Kyunghee University) ;
  • Jung Hyuk-Sang (Department of Neuroscience. Graduate School of East-West Medical Science, Kyunghee University) ;
  • Won Ran (Department of Neuroscience. Graduate School of East-West Medical Science, Kyunghee University) ;
  • Park Ji-Ho (Department of Neuroscience. Graduate School of East-West Medical Science, Kyunghee University) ;
  • Kang Chul hun (Department of Neuroscience. Graduate School of East-West Medical Science, Kyunghee University) ;
  • Sohn Nak-Won (Department of Neuroscience. Graduate School of East-West Medical Science, Kyunghee University)
  • Published : 2002.06.01
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Abstract

The current study was carried out to evaluate neuroprotective effects of Citri Pericarpium after transient global ischemia in gerbils. Male Mongolian gerbils weighing 60-80g were anesthetized with 2% isoflurane mixed with 30% oxygen and 70 % nitrogen. Bilateral common carotid arteries were occluded for 5 minute with microaneurysm dips. On 3 or 7 days after ischemic surgery, the gerbils were sacrificed. The brain were removed, embedded in paraffin and sectioned at 8㎛-thickness. Gerbils that received ischemic insult for 5 min showed extensive neuronal damage in the hippocampal CA1 region, and the number of viable neuronal cell was 51.0±2.5/mm, 32.2% of normal group at 7 days after ischemic surgery. In animals that underwent the extract of Citri Pericarpium treatment, the number of viable neuronal cell were significantly better preserved at 110.58±3.58/mm, 72.0% of normal group than those of ischemic group (P<0.01). In the immunohistochemistry of Bax and Bcl-2, the Citri Pericarpium treated group down-regulated the expression of Bax protein at 72hr after transient global ischemia. In contrast, Bcl-2 protein level was not changed. The appearance in TUNEL assay is similar to the pattern of Bax protein. The water extract of Citri Pericarpium significantly reduced the number of TUNEL-positive CA1 pyramidal neurons at 72hr. The results suggest that Citri Pericarpium has potential neuroprotective effects in the transient global ischemia and the increase in the ratio of Bcl-2 to Bax may contribute to the anti-apoptotic effect of Citri Pericarpium.

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