Journal of Animal Science and Technology

Korean Society of Animal Sciences and Technology (한국축산학회)
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Effects of Supplementary Yeast Culture(Saccharomyces cerevisiae, Pichia pastoris) on the Performance Small Intestinal Microflora and Serum IgG Concentration in Broiler Chickens

Yeast Culture(Saccharomyces cerevisiae, Pichia pastoris)가 육계의 생산성, 소장내 미생물 균총 및 혈청 IgG 농도에 미치는 영향

  • Park, D.Y. (Department of Animal Science and Technology, College of Industrial Science) ;
  • NamKung, H. (Department of Animal Science and Technology, College of Industrial Science) ;
  • Baek, I.K. (Department of Animal Science and Technology, College of Industrial Science)
  • 박대영 (중앙대학교 산업과학대학 동물자원과학과) ;
  • 남궁환 (중앙대학교 산업과학대학 동물자원과학과) ;
  • 백인기 (중앙대학교 산업과학대학 동물자원과학과)
  • Published : 2002.06.30
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Abstract

A broiler experiment was conducted to investigate the effect of supplementing yeast culture (Saccharomyces cerevisiae, Pichia pastoris) on the growth performance, small intestinal microflora and immune response in broiler chickens. One thousand hatched broiler chickens(Ross$^{(R)}$) were assigned to 6 treatments: control (basal diet), CTC; chlorotetracycline 100ppm, YC-SC; yeast culture(Saccharomyces cerevisiae) 0.3%, YC-PP; yeast culture(Pichia pastoris) 0.3%, RPPC-0.1; refined Pichia pastoris culture 0.1%, RPPC-0.3; refined Pichia pastoris culture 0.3%. There were no significant differences in growth, feed intake, feed efficiency and mortality among the treatments. However, chickens fed diets with yeast cultures showed numerically higher weight gain than those fed the control diets. Supplementation of yeast cultures and CTC improved feed efficiency and decreased mortality compared to control. Nutrient digestibilities were not affected by the dietary treatments. Total number of Lactobacilli in small intestine was higher while that of Cl. perfringens was lower with yeast culture treatments than control. Small intestine E. coli population of RPPC-0.3 treatment was significantly lower than that of the control. The serum IgG concentration tended to be higher in broilers fed yeast cultures than those fed the control and CTC diet. In conclusion, the supplementation of yeast culture products showed, although not significant but, numerical advantages in productivity and profile of microbial flora and serum IgG compared to the control and CTC supplementation.

본 시험은 yeast culture제제들(Saccharomyces cerevisiae, Pichia pastoris)의 첨가가 육계의 성장, 장내 미생물균총 및 혈청 IgG농도에 미치는 영향을 측정하기 위하여 기초사료(control)에 chlorotetracycline 100ppm(CTC), Saccharomyces cerevisiae 0.3%(YC-SC), Pichia pastoris 0.3% (YC-PP), 정제된 Pichia pastoris culture 0.1% (RPPC-0.1) 및 0.3%(RPPC-0.3)를 각각 첨가하였다. Ross 종 육계 1000수(암․수 각각 500수)를 20개의 floor pen에 50수(암 수 각 25수)씩 수용하여 control, CTC, YC-SC와 RPPC-0.1구는 3반복으로 YC-PP와 RPPC-0.3구는 4반복으로 배치하였다. 증체량은 처리간에 유의한 차이가 없었으나 대조구나 항생제첨가구에 비해 효모배양물의 첨가구(YC-SC, RPPC-0.1 및 RPPC- 0.3)에서 증체량이 약 4-5% 개선되는 효과가 있었다. 항생제와 효모배양물은 첨가한 구가 대조구에 비해 사료효율이 개선되며 폐사율이 감소하는 경향이 있었다. 영양소 이용율은 yeast culture 첨가에 의해 유의한 영향을 받지 않았다. 소장하부 내용물의 Lactobacilli의 수는 효모배양물의 첨가구(YC-SC, RPPC-0.1 및 RPPC-0.3)에서 대조구보다 다소 높은 경향을 보였고, Cl. perfringens의 수는 CTC와 정제된 Pichia pastoris culture의 첨가에 의해 뚜렷이 저하되었다. E. coli의 수는 정제된 Pichia pastoris 구들이 낮았는데 특히 RPPC-0.3가 대조구보다 유의하게(P$\prec$0.02) 낮았다. 혈청 IgG 농도는 처간에 유의한 차이는 없었으나 효모배양물의 첨가구들에서 control구나 항생제 첨구에서보다 높은 경향을 나타내었다. 결론적으로 본시험에 사용된 yeast culture제제는 생산성, 소장내 미생물 균총과 혈청 IgG에 있어서 유의한 차이는 없었으나 항생제(CTC)와 같거나 그 이상의 개선효과를 나타내었다.

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