Clinical and Experimental Reproductive Medicine

The Korean Society for Reproductive Medicine (대한생식의학회)
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Effects of Exposure of Propidium Iodide and Bisbenzimide on Differential Staining of Mouse Blastocysts

마우스 배반포 배의 Differential staining에서 Propidium Iodide와 Bisbenzimide의 노출이 미치는 영향

  • Park, Kee-Sang (Division of Life Resources, Daegu University) ;
  • Park, Sung-Baek (Division of Life Resources, Daegu University) ;
  • Lee, Taek-Hoo (Department of OB/Gyn, Kyungpook National University Hospital) ;
  • Chun, Sang-Sik (Department of OB/Gyn, Kyungpook National University Hospital) ;
  • Song, Hai-Bum (Division of Life Resources, Daegu University)
  • 박기상 (대구대학교 생명자원학부) ;
  • 박성백 (대구대학교 생명자원학부) ;
  • 이택후 (경북대학교병원 산부인과) ;
  • 전상식 (경북대학교병원 산부인과) ;
  • 송해범 (대구대학교 생명자원학부)
  • Published : 2002.12.30
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Abstract

Objective: These experiments were conducted to investigate the optimal expose length of propidium iodide (PI) and bisbenzimide on differential staining of mouse blastocysts. Materials and methods: A total 964 blastocysts (early${\sim}$hatched) was exposed to PI (n=831) (group I: $\leq$ 10; II: $11{\sim}15$; III: $16{\sim}20$; IV: $\geq$21 sec) and bisbenzimide (n=133) (group A: $\leq$1; B: $1{\sim}$3; C: $\geq$ 4 hr) in several periods for differential staining. Statistical analysis was performed using t-test with SigmaPlot-2001. P-values < 0.05 were accepted as statistically significant. Results: In case of PI exposure, differential staining rates were significantly higher (p<0.05) in group I (89.8%) than in any others (group II: 77.6%; III: 29.6%; IV: 22.2%) and higher (p<0.05) in group II than in group III and IV. In case of bisbenzimide exposure, differential staining rates were not statistically differences in three groups (group A: 97.4%; B: 87.8%; C: 93.3%). Conclusion: The differential staining rates of mouse blastocysts are not affected by the exposure length of bisbenzimide. However, blastocysts were exposed to PI with period of shorter than 15 sec show best outcomes of differential staining rates.

Keywords

References

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