Clinical and Experimental Reproductive Medicine

The Korean Society for Reproductive Medicine (대한생식의학회)
권호 표지

Expression of doc-1 in Pregnant Uterus of the Mouse

임신한 생쥐 자궁에서의 doc-1 발현

  • Cheon, Yong-Pil (Department of Biosciences, College of Veterinary Medicine, University of Illinois)
  • 전용필 (일리노이대학교 수의과대학 생물과학과)
  • Published : 2002.12.30
Download PDF
⟨ Previous Next ⟩

Abstract

Uterine cells carry out proliferation and differentiation for preparation the embryonic implantation during pregnancy. Therefore regulation of the cell proliferation is an essential step for uterine preparation, but there is not much information about the proliferation related genes in pregnant uterus. To identify these implantation specific genes, a PCR-select cDNA subtraction method was employed and got a few genes. One of the identified genes is a novel gene encoding oral tumor suppressor doc-1. To detect the doc-1 expression on the pregnant uterus, dot blotting, RT-PCR, and in situ hybridization were employed. Dot blotting revealed that doc-1 mRNA expression increase after implantation. During normal pregnancy, doc-1 mRNA expression was detected as early as day 1 of pregnancy with RT-PCR. Its expression was increased about 15 times after embryonic implantation. doc-1 transcript was localized in luminal epithelial cells but it was very faint during preimplantation. After starting the implantation, it localized in the stromal cells; heightened expression of doc-1 correlates with intense stromal cell proliferation surrounding the implanting blastocyst on day 6 morning. However in the decidualized cells, the intensity of localized doc-1 mRNA was weak. From those results, it is revealed that doc-1 express at pregnant uterus of the mouse. In addition it is suggested that doc-1 is the gene regulating the proliferation of the luminal epithelial cells and stromal cells during early implantation and decidualization.

Keywords

References

  1. Ferenczy A. Regeneration of the human endometrium. In: Genoglio CM, Wolff LM, editor. Progress in surgical pathology. New York: Masson; 1980. p.157-77
  2. Pedykula HA, Coles LG, McCracken JA, King NW, Jr Longcope C, Kaiserman-Abramof IR. A zonal pattern of cell proliferation and differentiation in the rhesus endometrium duringthe estrogen surge. BioI Reprod 1984; 32: 1103-18
  3. Leavitt WW. Cell biology of the endometrium. In: Wynn RM, Jollie WP, editor. Biology of the uterus. New York: Plenum Medical Book Company; 1989. p. 131-73
  4. Ben-Zimra M, Koler M, Melamed-Book N, Arensburg J, Payne AH, Orly J. Uterine and placental expression of steroidogenic genes during rodent pregnancy. Mol Cell Endo 2002; 187: 223-31 https://doi.org/10.1016/S0303-7207(01)00713-4
  5. Chantakru S, Miller C, Roach LE, Kuziel WA, Maeda N, Wang WC, et al. Contri- butions from self-renewal and trafficking to the uterine NK cell population of earlypregnancy. J Immun 2002; 168: 22-8 https://doi.org/10.4049/jimmunol.168.1.22
  6. Croy BA, Chankakru S, Esadeg S, Ashkar AA, Wei Q. Decidual natural killer cells: key regulators of placental development (a review). J Reprod Immun 2002;57: 151-68 https://doi.org/10.1016/S0165-0378(02)00005-0
  7. Robb L, Li R, Hartley L, Nandurkar HH, Koentgen F, Begley CG. Infertility in female mice lacking the receptor for interleukin 11 is due to a defective uterine response to implantation. Nat Med 1998;4:303-8 https://doi.org/10.1038/nm0398-303
  8. Xiao LJ, Chang H, Ding NZ, Ni H, Kadomatsu K, Yang ZM. Basigin expression and hormonal regulation in mouse uterus during peri-implantation period. Mol Reprod Dev 2002;63: 47-54 https://doi.org/10.1002/mrd.10128
  9. Ying Y, Zhao GQ. Detection of multiple bone morphogenetic protein messenger ribonucleic acidsand their signal transducer, Smad1, during mouse decidualization. BioI Reprod 2000; 63: 1781-9 https://doi.org/10.1095/biolreprod63.6.1781
  10. Abrahamsohn PA, Zorn TMT. Implantation and decidualization in rodents. J Exp Zool 1993; 266: 603-28 https://doi.org/10.1002/jez.1402660610
  11. Finn CA, McLaren A. A study of the early stages of implantation in mice. J Reprod Fert 1967; 13: 259-67 https://doi.org/10.1530/jrf.0.0130259
  12. Lim H, Paria BC, Das SK, Dinchuk JE, Langenbach R, Trzaskos JM, et al. Multiple female reproductive failures in cyclooxygenase 2-deficient mice. Cell 1997;91: 197-208 https://doi.org/10.1016/S0092-8674(00)80402-X
  13. Matsumoto H, Ma W, Dailoku T, Zhao X, Paria BC, Das SK, et al. Cyclooxygenase-2 differentially directs uterine angiogenesis during implantation in mice. J BioI Chem 2002; 277: 29260-7 https://doi.org/10.1074/jbc.M203996200
  14. Rider Y, Jones SR, Foster RT, ImakawaK. Changes in the temporal and spatial expression of H$\beta$58 during formation and maturation of the chorioallantoic placenta in the rat. BioI Reprod 2000; 63: 1735-46 https://doi.org/10.1095/biolreprod63.6.1735
  15. Benson GV, Lim H, Paria BC, Satokata I, Dey SK, Maas RL. Mechanisms of reduced fertility in Hoxa-10 mutant mice: uterine homeosis and loss of maternal Hoxa-10 expression. Development 1996; 122:2687-96
  16. Reese J, Brown N, Das SK, Dey SK. Expression of neu differentiation factor during the periimplantation period in the mouse uterus. BioI Reprod 1998; 58: 719-27 https://doi.org/10.1095/biolreprod58.3.719
  17. Bany B, Schultz GA. Peiotrophin messenger ribonucleic acid levels in mouse endometrial stromal cells during decidualizaiton. Endocronology 2001; 142:955-8 https://doi.org/10.1210/en.142.2.955
  18. Todd R, McBride J, Tsuji T, Donoff RB, Nagai M, Chou MY, et al. Deleted in oral cancer-I (doc-I), a novel oral tumor suppressor gene. FASEB J 1995; 9: 1362-70 https://doi.org/10.1096/fasebj.9.13.7557027
  19. Tsuji T, Duh FM, Latif F, Popescu NC, Zimonjic DB, McBride J, et al. Cloning, mapping, expression, function, and mutation analysis of the human ortholog of the hamster putative tumor suppressor gene doc-I. J BioI Chem 1998;2733: 6704-9
  20. Shintani S, Mihara M, Terakado N, NakaharaY, Matsumura T, Kohno Y, et al. Reduction of $p12^{DOC-1}$ expression is a negative prognostic indicator in patients with surgically resected oral squamous cell carcinoma. Clin Cancer Res 2001; 7: 2776-82
  21. Gordon HM, Kucera G, Salvo R, Boss JM. Tumor necrosis factor induces genes involved in inflammation, cellular and tissue repair, and metabolism in murine fibroblasts. J Immunol 1992; 148:4021-7
  22. Shintani S, Ohyarna H, Zhang X, McBride J, Matsuo K, Tsuji T, et al. $p12^{DOC-1}$ is a novel cyclin-dependent kinase 2-associated protein. Mol Cell BioI 2000; 20: 6300-7 https://doi.org/10.1128/MCB.20.17.6300-6307.2000
  23. Chen D, Xu X, Zhu LJ, Angervo M, Li Q, Bagchi MK, et al. Cloning and uterus/oviduct-specific expression of a novel estrogen-regulated gene (ERGI). J BioI Chem 1999;274: 32215-24 https://doi.org/10.1074/jbc.274.45.32215
  24. Dey SK. Implantation. In: Adashi EY, Rock JA, Rosenwaks Z, editor. Reproductive endocrinology, surgery and technology. New York: Lippincott-Raven Publishers; 1996. p. 421-34
  25. Ansell JD, Barlow PW, McLaren A. Binucleate and polyploid cells in the deciduas of the mouse. J Embryol Experi Morphol 1974;31: 223-7
  26. Das SK, Lim H, Paria BC, Dey SK. Cyclin D3 in the mouse uterus is associated with the dediualization process during early pregnancy. J Mol Endocrinol 1999;22: 91-101 https://doi.org/10.1677/jme.0.0220091
  27. Sachs L, Shelesnyak MC. The development and suppression of polyploidy in the developing and suppressed deciduoma in the rat. J Endocrinol 1995; 12: 146-51 https://doi.org/10.1677/joe.0.0120146
  28. Morgan DO. Principles of CDK regulation. Nature 1995;374: 131-4 https://doi.org/10.1038/374131a0
  29. Sherr CJ. Cancer cell cycles. Science 1996; 274:1672-7 https://doi.org/10.1126/science.274.5293.1672
  30. Hunter T, Pines J. Cyclins and cancer. II: Cyclin D and CDK inhibitors come of age. Cell 1994; 79:573-82 https://doi.org/10.1016/0092-8674(94)90543-6
  31. Murray AW. Creative blocks: cell-cycle checkpoints and feedback controls. Nature 1992; 359:599-604 https://doi.org/10.1038/359599a0
  32. Tan J, Raja S, Davis MK, Tawfik O, Dey SK, Das SK. Evidence for coordinated interaction of cyclin D3 with p21 and cdk6 in directing the development of uterine stromal cell decidualization and polyploidy during implantation. Mech Devel 2002; 111:99-113 https://doi.org/10.1016/S0925-4773(01)00614-1
  33. Hatakeyama S, Jensen JP, Weissman AM. Subcellular localization and ubiquitin-conjugation enzyme (E2) interactions of mammalian HECT family ubiquitin protein. J BioI Chem1997; 272: 15085-92 https://doi.org/10.1074/jbc.272.24.15085
  34. King RW, Deshaies RJ, Reters JM, Kirschner MW. How proteolysis drivesthe cell cycle. Science 1996;274: 1652-9 https://doi.org/10.1126/science.274.5293.1652
  35. Sherr CJ, Roberts JM. CDK inhibitors: positive and negative regulation of Gl-phase progression. Genes Dev 1999; 13: 1501-12 https://doi.org/10.1101/gad.13.12.1501