The Journal of Korean Medicine (대한한의학회지)

The Society of Korean Medicine (대한한의학회)
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The Effect of Woohwangcheongsim-won on Delayed Neuronal Death in Hypoxia

저산소증으로 유발된 지연성 신경세포사에 우황청심원이 미치는 영향

  • 김민석 (동의대학교 한의과대학 내과학교실) ;
  • 정승현 (동의대학교 한의과대학 내과학교실) ;
  • 신길조 (동의대학교 한의과대학 내과학교실) ;
  • 문일수 (동의대학교 한의과대학 해부학교실) ;
  • 이원철 (동의대학교 한의과대학 내과학교실)
  • Published : 2002.09.01
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Abstract

Objectives: The purpose of this investigation was to evaluate the effects of Woohwangcheongsim-won and to study the mechanism for neuronal death protection in hypoxia with Embryonic day 20 (E20) cortical cells of a rat (Sprague Dawley). Methods: E20 cortical cells were dissociated in neurobasal media and grown for 14 days in vitro (DIV). On 14 DIV, Woohwangcheongsim-won was added to the culture media for 24 hrs or 72 hrs. On 17 DIV, cells were given a hypoxic shock and further incubated in normoxia for another three days. On 20 DIV, Woohwangcheongsim-won's effects for neuronal death protection were evaluated by LDH assay, propidium iodide stain and phospho-H2AX immunostain and the mechanisms were studied by Bcl-2, Bak, Bax, caspase family, PKCα, ca1pain I. Results & Conclusions : 1. This study indicated that Woohwangcheongsim-won's effects for neuronal death protection in hypoxia were confirmed by LDH assay, propidium iodide stain and phospho-H2AX immunostain in culture method of Embryonic day 20(E20) cortical neuroblasts. 2. Woohwangcheongsim-won's mechanisms for neuronal death protection in hypoxia are to reduce the membrane damage fraction, to restrain DNA truncate, to restrain inflow of cytochrome c into cellularity caused by Bak diminution, to reduce the caspase cascade intiator caspase-8 and the effector caspase-3, to reduce the calpain I activity and to increase PKCand its activity in the membrane fraction. (J Korean Oriental Moo 2002;23(3):145～163)

Keywords

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