The Korean Journal of Ecology

The Ecological Society of Korea (한국생태학회)
권호 표지
DOI QR코드

DOI QR Code

Effect of 2,4,5-Trichlorobiphenyl (PCB-29) on Oxidative Stress and Activities of Antioxidant Enzymes in Tomato Seedlings

  • Published : 2002.12.01
Download PDF
⟨ Previous Next ⟩

Abstract

Leaves of two-week old seedlings of tomato (Lycopersicon esculentum) were treated with various concentrations (0, 0.2 and 0.4 $\mu$g/1) of 2,4,5-trichlorobiphenyl (PCB-29) and subsequent growth of seedlings, symptoms of oxidative stress and activities of antioxidant enzymes were investigated. Compared with the non-treated control, foliar application of PCB-29 decreased both biomass and superoxide ($O_2$) radical production but increased hydrogen peroxide production and lipid peroxidation such as malondialdehyde (MDA) formation with increased activities of superoxide dismutase (SOD), ascorbate peroxidase (APX) and guaiacol peroxidase (GPX). Further studies on the isozymes of SOD, peroxidase (POD) and APX showed that all three isozymes of SOD such as Mn-SOD, Fe-SOD and Cu/Zn-SOD, two among four isozymes of POD and all three isozymes of APX were selectively increased in response to PCB. Therefore, we suggest that a possible cause for the reduction of seedling growth by PCB exposure is the oxidative stress including over production of hydrogen peroxide and the selective expression of specific isozymes of some antioxidant enzymes.

Keywords

References

  1. Plant Physiol v.107 Dissection of oxidative stress tolerance using transgenic plants Allen,R.D. https://doi.org/10.1104/pp.107.4.1049
  2. Physiol. Plant. v.85 Ascorbate peroxidase - a hydrogen peroxide scavenging enzyme in plants Asada,K. https://doi.org/10.1111/j.1399-3054.1992.tb04728.x
  3. Appl. Environ. Microbiol. v.51 Rapid assay for screening and characterizing microorganisms for the ability to degrade polychlorinated biphenyls Bedard,D.;R.L.H.Unterman;Brennen, Bopp, J.;Haber,M.L.;Johnson,C.
  4. J. Biol. Chem. v.195 A spectrophotometric method for measuring the breakdown of hydrogen peroxide by catalase Beers,R.F.;I.W.Sizer
  5. Sci. Total Environ. v.250 The influence of a capacitor plant in Serpukhov on vegetable contamination by polychlorinated biphenyls Bobovnikova,Ts.I.;L.B.Alekseeva;A.V.Dibtseva;G.V.Chernik;D.B.Orlinsky;I.V.Priputina;G.A.Pleskachevskaya https://doi.org/10.1016/S0048-9697(00)00364-8
  6. Plant Sci. v.161 Water stress generates an oxidative stress through the induction of a specific Cu/Zn superoxide dismutase in Lotus comiculatus leaves Borsani,O.;P.Diaz;M.F.Agius;V.Valpuesta;J.Monza https://doi.org/10.1016/S0168-9452(01)00467-8
  7. Annu. Rev. Plant Physiol. Plant Mol. Biol. v.43 Superoxide dismutase and stress tolerance Bowler,C.;T.Van Montagu;D.Inze https://doi.org/10.1146/annurev.pp.43.060192.000503
  8. Cri. Rev. Plant Sci. v.13 Superoxide dismutase in plants Bowler,C.;W.Can Camp;M.Van Montagnu;D.Inze https://doi.org/10.1080/713608062
  9. Anal. Biochem. v.72 A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding Bradford,M.M. https://doi.org/10.1016/0003-2697(76)90527-3
  10. Plant Sci. v.161 The role of active oxygen species in plant signal transduction Breusegem,F.V.;E.Vranova;J.F.Dat;D.Inze https://doi.org/10.1016/S0168-9452(01)00452-6
  11. New Phytol. v.126 Role of superoxide, lipid peroxidation and superoxide dimutase in membrane perturbation during loss of viability in seeds of Shorea robusta Gaertn Chaitanya,K.S.K.;S.C.Naithani https://doi.org/10.1111/j.1469-8137.1994.tb02957.x
  12. Plant Sci. v.156 Mercury-induced oxidative stress in tomato seedlings Cho,U.H.;J.O.Park https://doi.org/10.1016/S0168-9452(00)00227-2
  13. J. Exp. Bot. v.32 Leaf senescence correlated with increased levels of membrane permeability and lipid peroxidation and decreased levels of superoxide dismutase and catalase Dhindsa,R.S.;P.Dhindsa;T.A.Thorpe https://doi.org/10.1093/jxb/32.1.93
  14. Plant Physiol. v.113 Responses of antioxidants to paraquat in pea leaves Donahue,J.;C.Moses Okpodu;C.Cramer;E.Grabau;E.Grene Alscher https://doi.org/10.1104/pp.113.1.249
  15. Nature v.341 The pine needles as a monitor of atmospheric pollution Eriksson,G.;G.Jensen;H.Kylin https://doi.org/10.1038/341042a0
  16. Bull. Environ. Contam. Toxicol. v.66 Disappearance of polychlorinated biphenyls (PCBs) when incubated with tissue cultures of different plant species Estime,L.;Jr.J.P.Rier https://doi.org/10.1007/s001280061
  17. Plant v.199 Chilling, oxidative stress and antioxidant responses in shoot cultures of rice Fadzilla,N.M.;V.Gill;R.P.Pinch;R.H.Burdon
  18. Biotech. Lett. v.9 Polychlorinated biphenyl (PCB) metabolism by plant cells Fletcher,J.;A.W.Groeger;J.McCardy;J.McFarlane https://doi.org/10.1007/BF01028290
  19. Antioxidants in Higher Plants Ascorbic acid Foyer,C.H.;R.C.Alscher(ed.);J.L.Hess(ed.)
  20. Physiol. Plant. v.92 Photooxidative stress in plants Foyer,C.H.;M.Lelandais;K.J.Kunert https://doi.org/10.1111/j.1399-3054.1994.tb03042.x
  21. Arch. Biochem. Biophys. v.247 Biological effects of the superoxide radical Fridovich,I. https://doi.org/10.1016/0003-9861(86)90526-6
  22. Free Radicals in Biology and Medicine Halliwell,B.;J.M.C.Gutteridge
  23. Toxicology v.145 The relative abilities of TCDD and its congeners to induce oxidative stress in the hepatic and brain tissues of rats after subchronic exposure Hassoun,E.A.;Li, Feng;A.Abushaban;S.J.Stohs https://doi.org/10.1016/S0300-483X(99)00221-8
  24. Physiol. Plant v.89 Salt-induced oxidative stress mediated by activated oxygen species in pea leaf mitochondria Hernandez,J.A.;F.J.Corpas;M.Gomez;L.A.del Rio;F.Sevilla https://doi.org/10.1111/j.1399-3054.1993.tb01792.x
  25. Science v.240 DNA damage and oxygen radical toxicity Imlay,J.A.;S.Linn https://doi.org/10.1126/science.3287616
  26. J. Agr. Food Chem. v.19 Absorption and translocation of root and foliage applied 2,4-dichlorophenol, 2,7-dichlorodibenzo-p-dioxin, and 2,3,7,8-tetrachlorodibenzo-p-dioxin Isensee,A.R.;G.E.Jones https://doi.org/10.1021/jf60178a045
  27. Plant Physiol. v.116 Oxidative damage in pea plants exposed to water deficit or paraquat Iturbe-Ormaetxe,I.;P.R.Escuredo;C.Arrese-Igor;M.Bacana https://doi.org/10.1104/pp.116.1.173
  28. Chemosphere v.24 Atmospheric pollution by persistent organic compounds: monitoring with pine needles Jensen,S.;G.Eriksson;H.Kylin https://doi.org/10.1016/0045-6535(92)90396-9
  29. Envron Res Sec A v.80 PCB congener pattern in rats consuming diets containing Great Lakes Salmon: analysis of fish, diets, and adipose tissue Jordan,A.J.;M.M.Feeley
  30. Biochem. Biophys. Acta. v.440 The effect of hydrogen peroxide on CO₂fixation of isolated intact chloroplast Kaiser,W.
  31. Free Radic. Res. v.31 Differential gene expression of rice superoxide dismutase isoforms to oxidative and environmental stresses Kamikana,H.;S.Morita;M.Tokumoto;T.Masamura;K.Tanka https://doi.org/10.1080/10715769900301541
  32. Eur. J. For. Pathol. v.4 The use of peroxidase activity for monitoring and mapping air pollution areas Keller,T. https://doi.org/10.1111/j.1439-0329.1974.tb00407.x
  33. Bull. Environ. Contam. Toxicol. v.66 Polychlorinated biphenyls and organochlorine pesticides in vegetation samples collected in Croatia Krauthacker,B.;S.H.Romanic;E.Reiner https://doi.org/10.1007/s00128-001-0010-5
  34. Plant Sci. v.159 Chilling stress-induced changes of antioxidant enzymes in the leaves of cucumber: in gel enzyme activity assays Lee,D.H.;C.B.Lee https://doi.org/10.1016/S0168-9452(00)00326-5
  35. Handbook of detection of enzymes on electrophoresis gels Manchenko,G.P.
  36. Plant Sci. v.127 Response of antioxidant enzymes to excess copper in tomato (Lycopersicon esculentum Mill) Mazhoudi,S.;A.Chaoui;M.H.Ghorbal;E.E.Ferjani https://doi.org/10.1016/S0168-9452(97)00116-7
  37. J. Biol. Chem. v.244 Superoxide dismutase: an enzymatic function for erythrocuprein (hemocuprein) McCord,J.M.;I.Fridovich
  38. Plant Physiol v.122 Iron-superoxide dismutase expression in transgenic alfalfa increases winter survival without a detectable increase in photosynthetic oxidative stress tolerance McKersie,B.D.;J.Murnaghan;K.S.Jones;S.R.Bowley https://doi.org/10.1104/pp.122.4.1427
  39. Physiol. Plant. v.95 Development of antioxidative defense system of wheat seedlings in response to high light Mishra,N.P.;T.Fatma;G.S.Singhal https://doi.org/10.1111/j.1399-3054.1995.tb00811.x
  40. Plant J. v.5 Regulation of pea cytosolic ascorbate and other antioxidant enzymes during the progression of drought stress and following recovery from drought Mittler,R.;A.Zilinskas https://doi.org/10.1111/j.1365-313X.1994.00397.x
  41. Plant Cell Physiol. v.22 Hydrogen peroxide is scavenged by ascorbate specific peroxidase in spinach chloroplasts Nakano,Y.;K.Asada
  42. Plant Cell Physiol. v.35 The influence of apoplastic ascorbate on the activities of cell-wall associated peroxidase and NADH-oxidases in needles of Noeway spruce (Picea abies L.) Otter,T.;A.Polle https://doi.org/10.1093/oxfordjournals.pcp.a078717
  43. Environ. Sci. Technol. v.30 PCBs in Lake Michigan water revisited Pearson,R.F.;K.C.Hornbuckle;S.J.Eisenreich;D.L.Swackhamer https://doi.org/10.1021/es940626n
  44. Oxidative Stress and the Molecular Biology of Antioxidants Defense Defense against photooxidative damage in plants Polle,A.;J.Scandalios(ed.)
  45. Env. And Exp. Bot. v.44 Changes in peroxidase activity and isozymes in spruce needles after exposure to different concentrations of cadmium Radotic,K.;T.Ducic;D.Mutavdzic https://doi.org/10.1016/S0098-8472(00)00059-9
  46. Physiol. Plant. v.72 Toxic oxygen species and protective systems of the chloroplast Salin,M.L.
  47. Plant Sci. v.161 Effect of cadmium on lipid peroxidation, superoxide anion generation and activities of antioxidant enzymes in growing rice seedlings Shah,K.R.;G.Kumar;R.G.S.Verma;R.S.Dubey https://doi.org/10.1016/S0168-9452(01)00517-9
  48. Adv. Exp. Med. Biol. v.283 Evidence for the induction of an oxidative stress in rat hepatic mitochondria by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) Stohs,S.J.;N.Z.Alsharif;M.A.Shara;Z.A.Al-Bayati;Z.Z.Wahba https://doi.org/10.1007/978-1-4684-5877-0_115
  49. Plant Sci. v.161 Changes in the antioxidant enzyme efficacy in two high yielding genotypes of mulberry (Morus alba L.) under NaCl salinity Sudhakar,C.;A.Lakshmi;S.Giridarakumar https://doi.org/10.1016/S0168-9452(01)00450-2
  50. New Phytol. v.105 The role of free radicals in senescence and wounding Thompson,J.E.;R.L.Legge;R.L.Barber https://doi.org/10.1111/j.1469-8137.1987.tb00871.x
  51. J. Plant Physiol. v.149 Lipid peroxidation in sorghum and sunflower seedlings as affected by ascorbic acid, benzoic acid and propyl gallate Zhang,J.;M.B.Kirkham https://doi.org/10.1016/S0176-1617(96)80323-3