Resveratrol Induces the Apoptosis of Osteosarcoma Saos-2 Cells

레스베라트롤에 의한 골육종 Saos-2 세포의 세포고사

  • 이현장 (원광대학교 의과대학 미생물학교실) ;
  • 양재현 (원광대학교 의과대학 미생물학교실) ;
  • 최익준 (원광대학교 의과대학 미생물학교실) ;
  • 최이천 (원광대학교 의과대학 미생물학교실) ;
  • 김용권 (원광대학교 의과대학 미생물학교실) ;
  • 임창인 (원광대학교 의과대학 미생물학교실) ;
  • 윤재도 (원광대학교 의과대학 미생물학교실) ;
  • 김호찬 (원광대학교 의과대학 미생물학교실) ;
  • 원진숙 (전북대학교 의과대학 생화학교실)
  • Published : 2002.09.01

Abstract

Resveratrol, a phytoalexin found in grapes, berries, and peanuts, is one of the most promising agents for cancer prevention. Recent studies show that the antitumor activity of resveratrol occurs through p53-mediated apoptosis. This study demonstrated the mechanism that resveratrol induced apoptosis in human osteosarcoma Saos-2 cells lacking p53. Treatment of osteosarcoma Saos-2 cells with resveratrol resulted in decrease of cell viability, which was revealed as apoptosis characterized by activation of caspase-3 protease as well as cleavage of poly(ADP-ribose) polymerase (PARP) with change of mitochondrial membrane potential transition. These results suggest that resveratrol may be potentially useful to treat osteosarcoma via activation of caspase protease and mitochondrial dysfunction.

Keywords

References

  1. Eur. J. Cancer v.33 no.11 On the current management of osteosarcoma. A critical evaluaion and a proposal for a modified treatment strategy Bruland,O.S.;Pihl,A https://doi.org/10.1016/S0959-8049(97)00252-9
  2. Hosp Pract(Off Ed.) v.28 no.12 Apoptosis: the physiologic pathway of cell death Cohen,J.J.
  3. J. Clin. Oncol. v.12 no.2 Prognostic factors in osteosarcoma:a critical Davis,A.M.;Bell,R.S.;Goodwin,P.J. https://doi.org/10.1200/JCO.1994.12.2.423
  4. FEBS Lett. v.421 no.3 Resveratrol, a remarkable inhibitor of ribonucleotide reductase Fontecave,M.;Lepoivre,M.;Elleingand,E.;Gerez,C.;Guittet,O. https://doi.org/10.1016/S0014-5793(97)01572-X
  5. Journal of Surgical Oncology v.4 no.5 The therapy of osteogenic sarcoma: current status and thoughts for the future Friedman,M.A.;Carter,S.K. https://doi.org/10.1002/jso.2930040512
  6. Drugs Exp. Clin. Res. v.25 no.2-3 Cancer chemopreventive activity of resveratrol Jang,M.;Pezzuto,J.M.
  7. Science v.275 no.5297 Cancer chemopreventive activity of resveratrol, a natural product derived from grapes Jang,M.;Cai,L.;Udeani,G.O.;Slowing,K.V.;Thomas,C.F.;Beecher,C.W.;fong,H.H.;Farnsworth,N.R.;Kinghorn,A.D.;Mehta,R.G.;Moon,R.C.;Pezzuto,J.M. https://doi.org/10.1126/science.275.5297.218
  8. J. Biol. Chem. v.272 no.49 Nitric oxide inhibits apoptosis by preventing increases in caspase-3-like activity via two distinct mechanisms Kim,Y.M.;Talanian,R.V.;Billiar,T.R. https://doi.org/10.1074/jbc.272.49.31138
  9. FEBS Lett. v.499 no.1-2 pRb suppresses camptothecin-induced apoptosis in human osteosarcoma Saos-2 cells by inhibiting c-Jun N-terminal kinase Lauricella,M.;Calvaruso,G.;Carabillo,M.;D'anneo,A.;Giuliano,M.;Emanuels,S.;Vento,R.;Tesoriere,G. https://doi.org/10.1016/S0014-5793(01)02553-4
  10. Cell. v.88 no.3 p53, the cellular gatekeeper for growth and division Levine,A.J. https://doi.org/10.1016/S0092-8674(00)81871-1
  11. Cell. v.74 no.6 p53-dependent apoptosis modulates the cytotoxicity of anticancer agents Lowe,S.W.;Ruley,H.E.;Jacks,T.;Housman,D.E. https://doi.org/10.1016/0092-8674(93)90719-7
  12. Nature v.362 no.6423 p53 is required for radiation-induced apoptosis in mouse thymocytes Lowe,S.W.;Schmitt,E.M.;Smith,S.W.;Osborne,B.A.;Jacks,T. https://doi.org/10.1038/362847a0
  13. Int. J. Cancer v.94 no.5 Resveratrol induces colon tumor cell apoptosis independently of p53 and precede by epithelial differentiation, mitochondrial proliferation and membrane potential collapse Mahyar-Roemer,M.;Katsen,A.;Mestres,P.;Roemer,K. https://doi.org/10.1002/ijc.1516
  14. Pediatr Clin. North. Am. v.44 no.4 Osteosarcoma Meyers,P.A.;Gorlick,R. https://doi.org/10.1016/S0031-3955(05)70540-X
  15. Int. J. Oncol. v.12 no.4 Antiproliferative effect of synthetic resveratrol on human breast epithelial cells Mgbonyebi,O.P.;Russo,J.;Russo,I.H.
  16. J. Pediatr. Hematol. Oncol. v.22 no.1 Osteosarcoma associated with absent thumbs: a report of two cases Orme,L.;Gorlick,R.;Meyers,P.A.;Athanasian,E.;Huvos,A.G. https://doi.org/10.1097/00043426-200001000-00014
  17. Leuk. Lymphoma. v.40 no.5-6 Resveratrol-from the bottle to the bedside? Pervaiz,S. https://doi.org/10.3109/10428190109097648
  18. Exp. Cell. Res. v.252 no.1 Paclitaxel induces apoptosis in Saos-2 cells with CD95L upregulation and Bcl-2 phosphorylation Pucci,B.;Bellincampi,L.;Tafani,M. Masciullo,V.;Melino,G.;Giordano,A. https://doi.org/10.1006/excr.1999.4591
  19. Biochem. Biophys. Res. Commun. v.250 no.1 Resveratrol arrests the cell division cycle at S/G2 phase transition Ragione,F.D.;Cucciolla,V.;Borriello,A.;Pietra,V.D.;Racioppi,L.;Soldati,G.;Manna,C.;Galletti,P.;Zappia,V. https://doi.org/10.1006/bbrc.1998.9263
  20. Eur. J. Biochem. v.254 no.3 Apoptosis signaling by death receptors Schulze-Osthoff,K.;Ferrari,D.;Los,M.;Wesselborg,S.;Peter,M.E. https://doi.org/10.1046/j.1432-1327.1998.2540439.x
  21. Pathol. annu. v.17 Necrosis and apoptosis: distinct modes of cell death with fundamentally different significance Searle,J.;Kerr,J.F.;Bishop,C.J.
  22. Mutat. Res. v.496 no.1-2 Resveratrol, a natural phenolic compound, inhibits cell proliferation and prevents oxidative DNA damage Sgambato,A.;Ardito,R.;Faraglia,B.;Boninsegna,A.;Wolf,F.I.;Cittadini,A. https://doi.org/10.1016/S1383-5718(01)00232-7
  23. Cancer Res. v.61 no.4 Resveratrol-induced activation of p53 and apoptosis is mediated by extracellular-singnal-regulated protein kinases and p38 kinase She,Q.B.;Bode,A.M.;Ma,W.Y.;Chen,N.Y.;Dong,Z.
  24. Int. Rev. Cytol. v.68 Cell death: the significance of apoptosis Wyllie,A.H.;Kerr,J.F.;Currie,A.R. https://doi.org/10.1016/S0074-7696(08)62312-8