Toxicological Research

Korean Society of Toxicology & Korea Environmental Mutagen Society (한국독성학회)
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Evaluation of Local Lymph Node Assay as an Alternative Method for Skin Sensitization Potential in Baltic Mice

Balb/c 마우스에서 Local Lymph Node Assay(LLNA)를 01용한 피부 감작성 시험 대체시험법 연구

  • 이종권 (식품의약품안전청 국립독성연구원 면역독성과) ;
  • 황인창 (식품의약품안전청 국립독성연구원 면역독성과) ;
  • 박재현 (식품의약품안전청 국립독성연구원 면역독성과) ;
  • 김형수 (식품의약품안전청 국립독성연구원 면역독성과) ;
  • 정승태 (식품의약품안전청 국립독성연구원 면역독성과) ;
  • 엄준호 (식품의약품안전청 국립독성연구원 면역독성과) ;
  • 오혜영 (식품의약품안전청 국립독성연구원 면역독성과)
  • Published : 2002.06.01
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Abstract

Allergic contact dermatitis (skin sensitization) may be caused by a wide variety of chemicals. A murine local lymph node assay (LLNA) has been developed as an alternative to guinea pig models for assessing the contact sensitization potential of chemical. This study was carried out to evaluate the skin sensitization potential for chemicals in Balb/c mice by LLNA. Contact allergen, dinitrochlorobenzene (DNCB), respiratory allergen, toluene diisocyanate (TDI) and a weak allergen, $\alpha$-hexlycinnamaldehyde (HCA) were wed as positive chemicals and irritant, sodium lauryl sulfate(SLS) also wed as a reference chemical in this study. The weights of lymph node in the mice treated with DNCB, TDI, and HCA were increased compared to vehicle control. There was a significant increase in lymph node weight of mice treated with high concentration of SLS compared to vehicle control. The stimulation index (SI) of Lymph node cell in the mice treated with DNCB, TDI, and HCA revealed over three-fold increase compared to vehicle control by $3^H$-thymidine uptake. All allergens correctly identified in this LLNA study wing Balb/c mice. These results suggest that LLNA wing Balb/c mice could be a useful method for screening the allergenic potential of chemicals. The expression of IL-2 mRNA was slightly increased in draining auricular lymph node cell of the mice treated with TDI and HCA by RT-PCR. However the IL-2 levels in DNCB and SLS of treated animals were not significantly changed.

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References

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