Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
권호 표지

Antitumor Activity of Lactobacillus plantarum Cytoplasm on Teratocarcinoma-Bearing Mice

  • Kim, Ji-Yeon (Department of Food Science and Technology and School of Agricultural Biotechnology, Seoul National University) ;
  • Woo, Hee-Jong (College of Veterinary Medicine, Seoul National University) ;
  • Kim, Kyoung-Heon (Department of Food Science and Technology and School of Agricultural Biotechnology, Seoul National University) ;
  • Kim, Eung-Ryool (R&D Center, Maeil Dairy Industry Co. Ltd.) ;
  • Jung, Hoo-Kil (R&D Center, Maeil Dairy Industry Co. Ltd.) ;
  • Juhn, Ho-Nam (R&D Center, Maeil Dairy Industry Co. Ltd.) ;
  • Lee, Hyong-Joo (Department of Food Science and Technology and School of Agricultural Biotechnology, Seoul National University)
  • Published : 2002.12.01
Download PDF
⟨ Previous Next ⟩

Abstract

Potential antitumor activity of Lactobacillus plantarum cytoplasm was examined using F9 teratocarcinoma-bearing BALB/C mice. The cytoplasmic fraction of L. plantarum was separated by sonication followed by ultracentrifugation. The fraction at a dose of 100 or 200 mg/kg/day was orally administered for 7 consecutive days before or after tumor inoculation to 16 mice. As a control, heat-killed whole cell was used at a dose of 100 mg/kg/day. Upon oral administrations of both the cytoplasm and heat-killed whole cell, when performed after and before tumor inoculation, the survival of F9-bearing mice prolonged more effectively. Administration of the cytoplasm after tumor inoculation extended the average survival days by 30 and $40\%$ at daily dosages of 100 and 200 mg/kg/day, respectively. This result suggests that the cytoplasmic fraction of L. plantarum has strong antitumor activity against mouse F9 teratocarcinoma in vivo.

Keywords

References

  1. J. Microbiol. Biotechnol v.10 Anti-Helicobacter pytoli activity of Bifidobacterium spp. Bae,E.A.;D.H.Kim;M.J.Han
  2. Cancer Lett. v.147 Inhibitory effects of freeze-dried milk fermented by selected Lactobacillus bulgaricus strains on carcinogenesis induced by 1,2-dimethylhydrazine in rats and by diethylnitrosamine in hamsters Balansky,R.;B.Gyosheva;G.Ganchev;Z.Mircheva;S.Minkova;G.Georgiev https://doi.org/10.1016/S0304-3835(99)00287-6
  3. Cancer Lett. v.85 Non-immunologicaly-mediated cytotoxicity of Lactobacillus casei and its derivative peptidoglycan against tumor cell lines Fichera,G.A.;G.Giese https://doi.org/10.1016/0304-3835(94)90244-5
  4. J. Appl. Bacteriol. v.66 A review: Probiotics in man and animals Fuller,R. https://doi.org/10.1111/j.1365-2672.1989.tb05105.x
  5. FEMS Microbiol. Rev. v.87 Health and nutritional benefits from lactic acid bacteria Gilliland,S.E.
  6. J. Natl. Cancer Inst. v.64 Effect of Lactobacillus acidophilus dietary supplements on 1,2-dimethylhydrazine dihydtochloride-induced intestinal cancer in rats Goldin,B.R.;S.L.Gorbach https://doi.org/10.1093/jnci/64.2.263
  7. Nutr. Cancer. v.25 The effect of Lactobacillus rhamnosus GG on the initiation and promotion of DMH-induced intestinal tumors in the rat Goldin,B.R.;L.J.Gualtieri;R.P.Moor https://doi.org/10.1080/01635589609514442
  8. Int. J. Imnunother. v.7 Influence of long-term yogurt consumption in young adults Halpem,G.M.;G. Vruwing;J.Van de Water;C.L.Keen;M.E.Gershwin
  9. J. Gen. Microbiol. v.133 Antitumor activity of purified arabinogalactan-peptidoglycan complex of the cell wall skeleton of Rhodococcus lentifragmentus Hirai,O.;T.Fujitsu;J.Mori;H.Kikuchi;S.Koda;M.Fujioka;Y.Morimoto
  10. Biosci. Biotechnol. Biochem. v.61 Characterization of a water-soluble polysaccharide fraction with immunopotentiating activity from Bifidobacterium adolescentis M101-4 Hosono,A.;J.Lee;A.Ametani;M.Natsume;M.Hirayama;T.Adachi;S.Kaminogawa https://doi.org/10.1271/bbb.61.312
  11. J. Microbiol. Biotechnol. v.10 Comparative evaluation of probiotic activities of Bifidobacterium longum MK-G7 with commerical Bifidobacteria strains Jung,H.K.;E.R.Kim;G.E.Ji;J.H.Park;S.K.Cha;S.L.Juhn
  12. Proc. Soc. Exp. Biol. Med. v.207 Inhibitory effect of Bifidobacterium longum cultures on the azoxymethane-induced aberrant crypt foci formation and fecal bacterial β-glucuronidase Kulkarni,N.;B.S.Reddy https://doi.org/10.3181/00379727-207-43817
  13. J. Microbiol. Biotechnol. v.11 Purification and characterization of bacteriocin J105 produced by Lactococcus lactis ssp. lactis J105 isolated from Kimchi Kwak,G.S.;S.K.Kim;H.K.Jun
  14. Ind. J. Exp. Biol. v.27 A cytotoxic substance produced by a wild culture of Lactobacillus casei D-34 against tumor cells Manjunath,N.;B.Ranganathan
  15. Nutr. Cancer. v.35 A probiotic strain of L. acidophilus reduces DMH-induced large intestinal tumors in male Sprague-Dawley rats. McIntosh,G.H.;P.J.Royle;M.J.Playne https://doi.org/10.1207/S15327914NC352_9
  16. J. Microbiol. Biotechnol. v.10 Cytotoxicity of shikonin metabolites with biotransformation of human intestinal bacteria Min,B.S.;M.R.Meselhy;M.Hattori;H.M.Kim;Y.H.Kim
  17. Agric. Biol. Chem. v.47 Anti-tumor polysaccharide from Lactobacillus sp. Oda,M.;H.Hasegawa;S.Komatsu;M.Kanbe;F.Tsuchiya https://doi.org/10.1271/bbb1961.47.1623
  18. Cancer Res. v.53 Inhibitory effect of Bifidobacterium longum on colon, mammary and liver carcinogenesis induced by 2-amino-3-methylimidazole[4,5-flquinoline, a food mutagen. Reddy,B.S.;A.Rivenson
  19. Jpn. J. Cancer Res. v.79 Antitumor effect of normal microflora on Ehrlich Ascites Tumor. Sakamoto,K.;K.Konishi https://doi.org/10.1111/j.1349-7006.1988.tb00017.x
  20. Cancer Res. v.45 A mew morphologically characterized cell wall preparation (whole peptidoglycan) from Bifidobacterium infantis with a higher efficacy on the regression of an established tumor in mice. Sekine,K.;T.Toida;M.Saito;M.Kuboyama;T.Kawashima;Y.Hashimoto
  21. Nutr. Cancer. v.5 Effect of feeding fermented milk on the incidence of chemically-induced colon tumors in rats. Shakelford,L.A.;D.R.Rao;C.B.Chawan;S.R.Pulusani https://doi.org/10.1080/01635588309513793
  22. Carcinogenesis. v.18 Bifidobacterium longum, a lactic acid-producing intestinal bacterium, inhibits colon cancer and modulates the intermediate biomarkers of colon carcinogenesis. Singh,J.;A.Rivenson;M.Tomita;S.Shimamura;N.Ishibashi;B.S.Reddy https://doi.org/10.1093/carcin/18.4.833
  23. J. Food Prot. v.62 Proinflammatory cytokine and nitric oxide induction in murine macrophage by cell wall and cytoplasmic extracts of lactic acid bacteria Tejada-Simon,M.V.;J.Pestka https://doi.org/10.4315/0362-028X-62.12.1435
  24. Jpn. J. Urol. v.85 Influence of Lactobacillus casei on rat bladder carcinogenesis Tomita,K.;H.Akaza;K.Nomoto;T.Yokokura;H.Matsushita;Y.Honma;Y.Aso https://doi.org/10.5980/jpnjurol1989.85.655