Journal of Nutrition and Health

The Korean Nutrition Society (한국영양학회)
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The Interaction of the 5, 10-methylenetetrahydrofolate Reductase (MTHFR) Polymorphism with Folate and Vitamin $B_12$ and Serum Homocysteine Concentrations in Pregnant Women

임신부의 5, 10-methylenetetrahydrofolate Reductase (MTHFR) 유전자형과 엽산 및 비타민 $B_12$ 섭취량이 혈중 호모시스테인 수준에 미치는 영향

  • 김기남 (이화여자대학교 생활환경대학 식품영양학과) ;
  • 김영주 (이화여자대학교 의과대학 산부인과학교실) ;
  • 장남수 (이화여자대학교 생활환경대학 식품영양학과)
  • Published : 2002.12.01
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Abstract

Hyperhomocysteinemia, resulted from an interaction between the mutation of MTHFR gene and B vitamin deficiency, is suggested as a possible cause for complications and adverse outcomes of pregnancy. The purpose of the present study was to investigate the relationship between the intakes of B vitamins and serum homocysteine concentrations with the C677T mutation in the MTHFR genotypes in 135 normal pregnant women of 24-28 weeks of gestation. Dietary intake of B vitamins did not differ among the three genotypes, but the negative correlation between dietary folate intake and the serum homocysteine level was the strongest in the T/T type (r ＝ -0.249) than in other genotypes (C/T: r＝ -0.040, C/C:r＝ 0.126, p＜0.05). Among the subject with the T/T type, the pregnant women who consumed folate less than 50% of the RDA had higher serum homocysteine levels than those who consumed folate greater than 125% of the RDA (10.4$\pm$5.9 vs 7.0$\pm$1.5 $\mu$mol/L, p＜0.05). Serum homocysteine levels were higher in the women with micronutrient supplements than those with no supplements in the T/T type, but such relation was not present in the C/C or the C/T type. In conclusion, serum homocysteine concentrations were influenced by the interrelationship between the MTHFR polymorphisms and dietary folate intake or micronutrient supplementation.

Keywords

References

  1. Atherosclerosis v.119 Genetic and nongenetic factors for moderate hyperhomocysteinemia Kang SS;Wong PWK https://doi.org/10.1016/0021-9150(95)05648-3
  2. J Soc Gynecol Invest v.6 Methylenetetrahydrofolate reductase polymorphism, folate, and susceptibility to preeclampsia Power RW;Minich LA;Lykins DL;Ness RB;Crombleholme WR;Roberts JM https://doi.org/10.1016/S1071-5576(98)00052-5
  3. Trends in Pharmacol Sci v.22 Biological and clinical implications of the MTHER C677T polymorphism Ueland PM;Hustad S;Schneede J;Refsum H;Vollset SE
  4. Fertil Steril v.60 Hyperhomocysteinemia: a risk factor in woman with unexplained recurrent early pregnancy loss Wouters MG;Boers GH;Blom HJ;Trijbels FJ;Thomas CM;Borm GF;Steegers-Theunissen RP;Eskes TK https://doi.org/10.1016/S0015-0282(16)56282-7
  5. Am J Obstet Gynecol v.179 Effects of folic acid and vitamin B6 supplementation on women with hyperhomocysteinemia and a history of preeclampsia or fetal growth restriction Leena M;Riyazi N;de Vries JIP;Jakobs C;van Geijn HP;Dekker GA https://doi.org/10.1016/S0002-9378(98)70263-7
  6. Eur J Obstet Gynecol v.66 Hyperhomocysteinemia: a risk factor for placental abruption or infarction Goddijn-Wessel TAW;Wouters MGAJ;vanden Molen EF;Spuijbroek MDEH;Steeger-Theunissen RPM;Blom HJ;Boers GHJ;Eskes TKAB https://doi.org/10.1016/0301-2115(96)02383-4
  7. N Engl J Med v.326 Intrauterine growth retardation,perinatal death, and maternal homocysteine levels Burke G;Robinson K;Refsum H;Stuart B;Drumm J;Graham I https://doi.org/10.1056/NEJM199201023260112
  8. Lancet v.345 Homocysteine metabolism in pregnancies complicated by neural tube defects Mill JL;McPartlin JM;Kirke PN https://doi.org/10.1016/S0140-6736(95)90165-5
  9. Proc Natl Acad Sci USA v.93 Homcysteine induces congenital defects of the heart and neural tube: effect of folic acid Rosenquist TH;Ratashak SA;Selhub J https://doi.org/10.1073/pnas.93.26.15227
  10. Am J Clin Nutr v.71 Plasma total Homocysteine pregnancy complications and aderse pregnancy outcomes: the Hordaland Homocysteine Study Vollset SE;Refsum H;Irgens LM;Emblem BM;Tverdal A;Gjessing HK;Monsen ALB;Ueland PM
  11. Am J Clin Nutr v.69 Low-dose folec acid supplementation decreases plasma homocystiene concentrations: a randomized trial Brouwer IA;van Dusseldorp M;Thomas CMG;Duran M;Hautvast JGAJ;Eskes TKAB;Steegers-Theunissen RPM
  12. Am Coll Obstet Gynecol v.92 Plasma homocysteine concentrations in pregnant and nonpregnant woman with controlled folate intake Bonnette RE;Caudill MA;Boddie AM;Hutson AD;Kauwell GPA;Bailey LB
  13. J Nutr v.131 Polymorphisms of key enzymes in homocysteine metabolism affect diet responsiveness of plasma homocysteine in healthy woman Silaste ML;Rantala M;Sampi M;Alfthan G;Aro A;Kesaniemi A
  14. J Am Coll Cardiol v.38 Optimization of dietary folate or low-dose folic acid supplements lower homocystiene but do not enhance endothelial function in healthy adults, irres pective of the methylenetetrahydrofolate reductase(C677T)genotype Pullin CH;Ashfield-Watt PAL;Burr ML;Clark ZE;Lewis MJ;Moat SJ;Newcombe RG;Powers HJ;Whiting JM;McDowell IFW https://doi.org/10.1016/S0735-1097(01)01668-0
  15. Lancet v.349 Thermolabile variant of 5,10-methylenetertrahydrofolate reductase associated with low redcell folates: implications for folate intake recommendations Molloy AM;Daly S;Mills JL;Whitehead AS;Ramsbottom D;Conley MR;Weir DG;Scott JM https://doi.org/10.1016/S0140-6736(96)12049-3
  16. Korean J Nutrition v.34 no.7 comparison of folate intake and food sources in college students using the 6th vs. 7th nutrient database Hyun T;Han YH
  17. J Korean Soc Food Sci Nutr v.30 no.1 Major foods for folate and their folate contents of Korean child-bearing woman Jin HO;Lim HS
  18. J Chromatogr v.422 Determination of free and total homocysteine in human plasma by high performance liquid chromatography with fluorescence detection Araki A;Sako Y https://doi.org/10.1016/0378-4347(87)80438-3
  19. Nat Genet v.10 Candidate genetic risk factor for vascular disease: a common mutation in methylenetetrahydrofolate reductase Frosst P;Blom HJ;Milos R;Goyette P;Sheppard C;Matthew R;Boers GJ;den Heijer M;Kluijtmans LA;van den Heuvel LP;Rozen RA https://doi.org/10.1038/ng0595-111
  20. Am J Clin Nutr v.71 Is homocysteine a biomarker for identifying women at risk of complications and adverse pregnancy outcomes? Picciano MF
  21. Korean J Nutr v.33 no.8 Serum folate levels of maternal-umbilical cord blood and pregnancy outcomes Ahn HS;Kim JS;Lee GJ;Kim YT
  22. Korean J Nutr v.31 no.8 Folate levels of umbilical cord blood and pregnancy outcomes Lim HS;Lee JA
  23. Korean J Nutr v.26 no.4 Effect of dietary folate intakes on serum folate levels of pregnant and lactation women Kang M;Chang NS
  24. J Nutr v.127 Folate status response to controlled folate intake in pregnant woman Caudill MA;Cruz AC;GregoryⅢ JF;Hutson AD;Bailey LB
  25. Korean J Nutr v.34 no.4 Serum vitamin $B_{12}$ levels of maternal-umbilical cord blood and pregnancy outcomes Ahn HS;Lee GJ;Hong HK;Chung SW;Yang JH;Chung HW
  26. J Clin Invest v.98 Determinants and vitamin responsiveness of intermediate hyperhomocysteinemia(>or = 40 micromol/liter).The Hordaland Homocysteine Study Guttormsen AB;Ueland PM;Nesthus I;Nygard O;Schneede J;Vollset SE;Refsum H https://doi.org/10.1172/JCI119024
  27. Am J Kideny Diseas v.39 no.5 Methylenetetrahydrofolate reductase genotype, vitamin $B_{12}$, and folate influence plasma homocsyteine in hemodialysis patients Nakamura T;Saionji K;Hiejima Y;Hirayama H;Tago K;Takano H;Tajiri M;Hayashi K;Kawabata M;Funamizu M;Makita Y;Hata A https://doi.org/10.1053/ajkd.2002.32779
  28. Eur J Clin Biochem v.30 Decreased serum homcoysteine in pregnancy Andersson A;Hultberg B;Brattstrom L;Isakssson A
  29. Am J Clin Nutr v.76 Methylenetetrahydrofolate reductase 677C→T genotype modulates homocysteine responses to a folate-rich diet of a low-dose folic acid supplement: a randomized controlled trial Ashfield-Watt PAL;Pullin CH;Whiting JM;Clark ZE;Moat SJ;Newcombe RG;Burr ML;Lewis MJ;Powers HJ;McDowell FW
  30. J Obstet Gynaecol Res v.27 no.6 The relation between plasma homocysteine concentration and methylenetetrahydrofolate reductase gent polymorphism in pregnant woman Murakami S;Matsubara N;Saitoh M;Miyakaw S;Shoji M;Kubo T https://doi.org/10.1111/j.1447-0756.2001.tb01284.x
  31. Korean J Genetics v.20 no.1 The C677T mutation in the methylenetetrahydrofolate reductase gene in Koreans Park KS;Podskarbi T;Yoo EA;Shin YS
  32. Br J Nutr v.85 Longitudinal vitaminand homocysteine levels in normal pregnancy Cikot RJLM;Steegers-Theunissen RPM;Thomas CMG;de Boo TM;MErkus HMWM;Steegers EAP https://doi.org/10.1079/BJN2000209
  33. Eur J Obstet Gynecol v.61 Changes of some vitamin levels during and after normal pregnancy Bruinse HW;van den Berg H https://doi.org/10.1016/0028-2243(95)02150-Q