Korean journal of applied entomology (한국응용곤충학회지)

Korean Society of Applied Entomology (한국응용곤충학회)
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Identification of a Pathogenic Bacterium, Staphylococcus gallinarum, to Bombyx mori

누에(Bombyx mori) 무름병을 야기하는 병원성 세균 Staphylococcus gallinarum의 동정

  • 김길호 (상주대학교 농학부 잠사곤충학과) ;
  • 박영진 (안동대학교 자연과학대학 농생물학과) ;
  • 김용균 (안동대학교 자연과학대학 농생물학과)
  • Published : 2002.12.01
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Abstract

Flacherie symptom was found in the fifth instar larvae of silkworm, Bombyx mori. The bacterial pathogen was isolated from the hemolymph of the infected silkworm and identified. The isolated bacteria caused a significant flacherie pathogenicity to the fifth instar larvae of B. mori when $5{\times}10^{6}$ cfu (colony-forming unit) of the bacteria was injected into each larva. The infected larvae began to die at 6 days after injection and resulted in complete mortality at 10 days. The bacterium was identified as Staphylococcus gallinarum based on the morphological and physiological characteristics described in Bergey's manual. This identification was further supported by the characters of carbohydrate utility analyzed from a bacterial identification system ($MicroLog^{\circledR}$) and also by the molecular structure of 165-23S rDNA internal transcribed spaces. As an insecticidal action, S. gallinarum caused hemolymph septicemia by its cytotoxic effect on the hemocytes of B. mori.

누에(Bombyx mori) 5령 유충에서 무름병 증상이 발견되었다. 세균성 병원균이 감염 누에 혈액에서 분리되었고, 본 연구에서 동정되었다. 분리된 세균을 각각의 누에 5령 유충에 $5{\times}10^{6}$ cfu(colony-forming unit) 농도로 혈강 주사되었을 때, 뚜렷한 무름병 증상을 유발시켰다. 이렇게 감염된 유충들은 접종 6일 후부터 죽기 시작하여, 10일 경과 후 처리된 유충 모두 사망하였다. Bergey의 세균동정집에 기록된 형태적 및 생리적 형질을 기준으로 이 곤충병원세균이 Staphylo-coccue gallinarum으로 판명되었다. 이 판정은 탄소원 분석 미생물동정장치($MicroLog^{\circledR}$) 분석 결과와 16S-23S rDNA internal transcribed space 구조에 의해서 확인되었다. 이 세균이 발휘하는 살충기작의 일환으로서, 감염 후 누에 유충 혈구에 세포치사 효과를 주어 패혈증을 유발하는 것으로 분석되었다.

Keywords

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