DOI QR코드

DOI QR Code

The Effect of NaCl on the Greening of Etiolated Leaves of Barely (Hordeum vulgare L.) Seedings

NaCl이 황백화된 보리(Hordeum vulgare L.) 잎의 녹화에 미치는 영향

  • Published : 2002.10.01

Abstract

The effects on photosynthesis of NaCl(0, 0.2, 0.4, 0.6, 0.8 or 1.0 M) were examined in etiolated barley seedlings. Chlorophyll(Chl) a, Chl b and carotenoid contents, Chl a fluorescence and quenching coefficients of Chl fluorescence have been determined in the primary leaves of etiolated barley seedlings cultivated under low light(60 $\mu$$m^{-2}\;s^{-1}$). Chl a, b, and carotenoid contents were decreased remarkably in comparison with the control at 0.4 M NaCl. However, the value of Fo and Fv were decreased at 0.6 M NaCl and the ratio of Fv/Fm were deceased at 1.0 M NaCl. Chlorophyll synthesis was seriously inhibited from 0.4 M NaCl, and the photosynthetic electron transport system was inhibited from 0.6 M NaCl. Quantum of photosystem II reaction center was inhibited at 1.0 M NaCl. The effects of NaCl on the Chl content were raised in a 6 hrs, but the effects of NaCl on the value of Fo, Fv and Fv/Fm were raised in 30 hrs. The value of qP was decreased in comparison with the control at all concentrations, but there was a small change in the value qE. These results provide evidence that NaCl inhibited effects of various concentration of NaCl were inhibited quinone redox, however, proton gradient between thylakoid membranes was little damaged.

Keywords

References

  1. Cheeseman, J.M., 1988, Mechanisms of salinity tolerance in plants, Plant Physiol., 87, 547-550. https://doi.org/10.1104/pp.87.3.547
  2. Szabolcs, I., 1989, Salt-affects soil, CRC Press, Boca Raton, FL., 120-143pp.
  3. Seemann, J.R. and C. Critchley, 1995, Effects of the salt stress on the growth, ion content, stomatal behaviour and photosynthetic capacity of a salt-sensitive species, Phaseolus vulgaris L. Planta, 164, 151-162.
  4. Baker, N.R., 1991, A possible role for photosystem II in environmental perturbations of photosynthesis, Physiol. Plant., 81, 563-570. https://doi.org/10.1111/j.1399-3054.1991.tb05101.x
  5. Bongi, G. F. and Loreto, 1989, Gas-exchange properties of salt-stressed olive(Olea europea L.) leaves, Plant Physiol., 90, 1408-1416. https://doi.org/10.1104/pp.90.4.1408
  6. Robinson, S.J. and C.F., 1982, Photosynthetic electron transfer in preparations of the cyanobacterium Spirulina platensis, Plant Physiol., 70, 154-161. https://doi.org/10.1104/pp.70.1.154
  7. Smillie, R. and R. Nott, 1982, Salt tolerance in crop monitored by chlorophyll fluorescence in vivo, Plant Physiol., 70, 1049-1054. https://doi.org/10.1104/pp.70.4.1049
  8. Chun, H.S., Y.M. Kwon, and C.B. Lee, 1993, Comparison of toxic effects of mercury, copper and zinc on photosystem II of barley chloroplasts, Korean J. Bot., 36(3), 195-201.
  9. Park, K.E. and H.S. Chung, 1997, Change of chlorophyll fluorescence by the greening of etiolated barley(Hordeum vulgare L.) seeding, Korean J. Bio., 12(1): 47-52.
  10. Park, K.E. and H.S. Chung, 1996, The effect of ozone on the greening of barley(Hordeum vulgare L.) seeding, Korean J. Environ. 5(4), 545-553.
  11. Hiscox, J.D. and G.F. Israelstam, 1979, A method for the extraction of chlorophyll from leaf tissue without maceration, Can. J. Bot., 57, 1332-1334. https://doi.org/10.1139/b79-163
  12. Arnon, D., 1949, Copper enzymes in isolated chloroplasts. Polyphenoloxidase m Beta vulgaris, Plant Physiol., 24, 1-15. https://doi.org/10.1104/pp.24.1.1
  13. Jensen, S.L. and A. Jensen, 1971, Quantitative determination of carotenoids in photosynthetic tissues. In Method in Enzymology. 23, Academic Press, New York, 586-602pp.
  14. Driesenaar, A.R.J., U. Schreiber, and S. Malkin, 1994, The use of photothermal radiometry in assessing leaf photosynthesis:II. Correlation of energy storage to photosystem II fluorescence parameters, Photosynth. Res., 40, 45-54. https://doi.org/10.1007/BF00019044
  15. Schreiber, U., 1986, Chlorophyll fluorescence assay for ozone injury in intact plants, Plant Physiol., 61, 80-84. https://doi.org/10.1104/pp.61.1.80
  16. Robinson, S.P., W.J. Downton, and J.A. Millhouse, 1983, Photosynthesis and ion content of leaves and isolated chloroplast of salt-stressed spinach, Plant Physiol., 73, 238-242. https://doi.org/10.1104/pp.73.2.238
  17. Bolhar-Nordenkamof and G. Oquist, 1993, Chlorophyll fluorescence as a tool in photosynthesis research., In Photosynthesis and Production in a Changing Environment, Hall, D.G., J.M.O. Scurlock, H.R. Bolhar-Nordenkampf, R.C. Leegood and S.P. Long(eds.), Clays Ltd., London, England, 194-206.
  18. Falk, S., J.W. Leverenz, G. Samuelsson, and G. Oquist, 1992, Changes in photosystem II fluorescence in Chlamydomonas reinhardtii exposed to increasing levels of irradiance in relationship to the photosynthetic response to light, Photosynth. Res., 31, 31-40. https://doi.org/10.1007/BF00049534
  19. Chung, H.S., B.Y. Moon, I.K. Chung, I.H. Park and C.H. Lee, 1993, Light-dependent chilling injury on the photosynthetic activities of cucumber cotyledons, Korean J. Bot., 36, 133-140.
  20. Hipkins, M.F. and N.R. Baker, 1986, Fluorescence kinetics, In Photosynthesis Energy Transduction; A practical Approach, M.F. Hipkins and N.R. Baker (eds.), IRL Press, Oxford, 87-99pp.
  21. Van Kooten, O. and J.F.H. Snel, 1990, The use of chlorophyll fluorescence nomonclature in plant stress physiology, Photosynth. Res., 25, 147-150. https://doi.org/10.1007/BF00033156
  22. Oxborough, K. and P. Horton, 1989, A study of the regulation and function of energy-dependent quenching in pea chloroplasts, Biochim. Biophy. Acta., 934, 135-143.
  23. Chung, H.S., S.D. Song, K.S. Roh, J.S. Song, and K.E. Park, 1999, The effects of acidic electrolytic water on the development of barley chloroplast, Korean J. Environ., 8(2), 247-253.
  24. Lu, C. and A. Vonshak, 1999, Characterization of PS II photochemistry in salt-adapted cells of cyanobacterium Spirulina platensis, New Phytol., 141, 231-239. https://doi.org/10.1046/j.1469-8137.1999.00340.x