Animal cells and systems

  • 제6권4호
  • /
  • Pages.323-326
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  • 2002
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  • 1976-8354(pISSN)
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  • 2151-2485(eISSN)
한국통합생물학회 (The Korean Society for Integrative Biology)
권호 표지

Initial Gene Expression Profile of Rainbow Trout(Oncorhynchus mykiss) Intestine

  • Kim, Soonhag (National Genome Research Institute, Korea National Institute of Health)
  • 발행 : 2002.12.01
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초록

One hundred and three random complementary DNA clones representing rainbow trout intestine were par1i811y sequenced as an approach to analyze the transcribed sequences of its genome. Of the sequences generated, 60.0% of the ESTs were represented by 40 known genes. Thirty-five clones of unknown gene products potentially represented 34 novel genes. The most Bbundantly represented messages were the 28S ribosomal protein (6.5%) and beta actin (5.8%). The genes involved in ribosome formation (18%) accounted for the major gene expression. Development of EST panels representing the genes expressed in a particular tissue will be useful in determining the role of these genes in normal function and in response to developmental, hormonal, environmental and physiological changes.

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참고문헌

  1. Adams MD, Kelley JM, Gocayne JD, Dubnick M, Polymeropoulos MH, Xiao H, Merril CR, Wu A, Olde B, Moreno RF, Kerlavage AR, McCombie WR, and Venter JC (1991) Complementary DNA sequencing: expressed sequence tags and human genome project. Science 252: 1651-1656 https://doi.org/10.1126/science.2047873
  2. Bishop JO, Morton JG, Rosebach M, and Richardson M (1974) Three abundance classes in Hela cell messenger RNA. Nature 250: 199-204 https://doi.org/10.1038/250199a0
  3. Liang P and Pardee AB (1992) Differential display of eukaryotic mRNA by means of the polymerase chain reaction. Science 257: 967-971 https://doi.org/10.1126/science.1354393
  4. Paabo S (2001) The human genome and our view of ourselves. Science 291: 1219-1220 https://doi.org/10.1126/science.1056972
  5. Patanjali SR, Parimoo S, and Weissman SM (1991) Construction of a uniform-abundance (normalized) cDNA library. Proc Natl Acad Sci USA 88: 1943-1947 https://doi.org/10.1073/pnas.88.5.1943
  6. Sanger F, Nicklen S, and Coulson AR (1977) DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74: 5463-5467 https://doi.org/10.1073/pnas.74.12.5463
  7. Sasaki YF, Ayusawa D, and Oishi M (1994) Construction of a normalized cDNA library by introduction of a semi-solid mRNA-cDNA hybridization system. Nucleic Acids Res 22: 987-992 https://doi.org/10.1093/nar/22.6.987
  8. Velculescu VE, Zhang L, Vogelstein B, and Kinzler KW (1995) Serial analysis of gene expression. Science 270: 484-487 https://doi.org/10.1126/science.270.5235.484