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Cloning and Phylogenetic Characterization of Coat Protein Genes of Two Isolates of Apple mosaic virus from ¡?Fuji¡? Apple

  • Lee, Gung-Pyo (Institute of Natural Science, Seoul Women′s University) ;
  • Ryu, Ki-Hyun (Plant Virus GenBank, Division of Environmental and Life Sciences, Seoul Women′s University) ;
  • Kim, Hyun-Ran (Horticultural Environment Division, National Horticultural Research Institute, Rural Development Administration) ;
  • Kim, Chung-Sun (Division of Bio0Industrial Science, College of Life and Environmental Sciences, Korea University) ;
  • Lee, Dong-Woo (Division of Bio0Industrial Science, College of Life and Environmental Sciences, Korea University) ;
  • Kim, Jeong-Soo (Horticultural Environment Division, National Horticultural Research Institute, Rural Development Administration) ;
  • Park, Min-Hye (Institute of natural Science, Seoul Women′s University) ;
  • Noh, Young-Mi (Division of Bio-Industrial Science, College of Life and Environmental Sciences, Korea University) ;
  • Choi, Sun-Hee (Division of Bio0Industrial Science, College of Life and Environmental Sciences, Korea University) ;
  • Han, Dong-Hyun (Division of Bio-Industrial Science, College of Life and Environmental Sciences, Korea University) ;
  • Lee, Chang-Hoo (Division of Bio-Industrial Science, College of Life and Environmental Sciences, Korea University)
  • 발행 : 2002.01.01

초록

Apple mosaic virus (ApMV), a member of the genus Ilarvirus, was detected and isolated from diseased 'Fuji' apple (Malus domestica) in Korea. The coat protein (CP) genes of two ApMV strains, denoted as ApMV-Kl and ApMV-K2, were amplified by using the reverse transcription and polymerase chain reaction (RT-PCR) and were analyzed thereafter. The objectives were to define the molecular variability of genomic information of ApMV found in Korea and to develop virus-derived resistant gene source for making virus-resistant trans-genic apple. RT-PCR amplicons for the APMVS were cloned and their nucleotide sequences were determined. The CPs of ApMV-Kl and ApMV-K2 consisted of 222 and 232 amino acid residues, respectively. The identities of the CPs of the two Korean APMVS were 93.1% and 85.6% at the nucleotide and amino acid sequences, respectively. The CP of ApMV-Kl showed 46.1-100% and 43.2-100% identities to eight different ApMV strains at the nucleotide and amino acid levels, respectively. When ApMV-PV32 strain was not included in the analysis, ApMV strains shared over 83.0% and 78.6% homologies at the nucleotide and amino acid levels, respectively. ApMV strains showed heterogeneity in CP size and sequence variability. Most of the amino acid residue differences were located at the N-termini of the strains of ApMV, whereas, the middle regions and C-termini were remarkably conserved. The APMVS were 17.(1-54.5% identical with three other species of the genus Ilarviyus. ApMV strains can be classified into three subgroups (subgroups I, II, and III) based on the phylogenetic analysis of CP gene in both nucleotide and amino acid levels. Interestingly, all the strains of subgroup I were isolated from apple plants, while the strains of subgroups II and III were originated from peach, hop, or pear, The results suggest that ApMV strains co-evolved with their host plants, which may have resulted in the CP heterogeneity.

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참고문헌

  1. Alrefai, R. H., Shiel, P. J., Domier, L. L., D'Arcy, C. J., Berger, P.H. and Korban, S. S. 1994. The nucleotide sequence ofapplemosaic virus coat protein gene has no similahty with other Bromoviridae coat protein genes. J. Gen. Virot. 75:2847-2850 https://doi.org/10.1099/0022-1317-75-10-2847
  2. Ansel-McKiimey, P. and Gehrke, L. 1998. RNA determinants of aspecific RNA-coat protein peptide interaction in alfalfamosaic virus: conservation of a homologous feature m Ilarvirus RNAs. J. Mol .BioI. 278:767-785 https://doi.org/10.1006/jmbi.1998.1656
  3. Aparicio, F. and Pallas, V. 2002. The molecular variability analysis of the RNA 3 of fifteen isolates of Prunus necrotic ringspotvirus sheds light on the minimal requirements for the synthesisof its subgenomic RNA. Virus Genes 25:75-84 https://doi.org/10.1023/A:1020126309692
  4. Bachman, E. J., Scott, S. W., Xin, G. and Vance, V. B. 1994. Thecomplete nucleotide sequence of prune dwarf Ilarvirus RNA3: implications for coat protein activation of genome replication in IIarviruses. Viroloey 201:127-131 https://doi.org/10.1006/viro.1994.1272
  5. Bachman, E. J., Scott, S. W., Xin, G. and Vance, V. B. 1994. Thecomplete nucleotide sequence of prune dwarf Ilarvirus RNA3: implications for coat protein activation of genome replication in IIarviruses. Viroloey 201:127-131
  6. Fulton, R. W. 1972. Apple mosaic vims. CMI/AAB Descriptionsof Plant Vimses, No. 83., UK
  7. German-Retana, S., Bergey, B., Delbos, R. P, Candresse, T. andDunez, J. 1997. Complete nucleotide sequence ofthe genome of a severe cherry isolate of apple chlorotic leaf spot trichovirus (ACLSV). Arch. ViroI 142:833-841 https://doi.org/10.1007/s007050050122
  8. Guo, D., Maiss, E., Adam, G. and Casper, R. 1995. Prunusnecrotic ringspot Ilarvirus: nucleotide sequence of RNA 3 andthe relationship to other lIarvitvses based on coat protein comparison. J. Gen. Virol. 76:1073-1079 https://doi.org/10.1099/0022-1317-76-5-1073
  9. Hammond, R. W. and Crosslin, J. M. 1995. The complete nucleotide sequence of RNA 3 of a peach isolate of Prunus necroticringspot virus. Virology 208:349-353 https://doi.org/10.1006/viro.1995.1159
  10. Jung, H. J., Ueda, S., Ryu, K. H., Lee, S. Y. and Choi, J. K. 2000.A novel strain of Cucumber mosaic virus isolated from Lilium longifIorum. Plant Pathol. J. 16:306-311
  11. Martelli, G. P. and Jelkmann, W. 1998. Foveavirus, a new plantvirus genus. Arch. Virol. 143:1245-1249 https://doi.org/10.1007/s007050050372
  12. Mink, G. I., Howell, W. E., Cole, A. and Regev, S. 1987. Threeserotypes of prunus necrotic ringspot virus isolated from rugose mosaic-diseased sweet cherry in Washington. Ptant Dis.71:91-93 https://doi.org/10.1094/PD-71-0091
  13. Petrzik, K. and Lenz, O. 2002. Remarkable varIability of apple mosaic virus capsid protein gene after nucleotide position 141.Arch. Virol. 147:1275-1285 https://doi.org/10.1007/s00705-002-0819-1
  14. Rybicki, E. P. 1995. The Bromoviridae. In: Virus Taxonomy. SixthReport of the Intemational Committee on Taxonomy of Viruses, pp. 450-457. ed. by F. A. Murphy, C. M. Fauquet, D.H. L. BishoP, S. A. Ghabrial, A. W. Jarvis, G. P. Martelli, M.A. Mayo and M. D. Summers. Springer-Verlag, NY
  15. Ryu, K.. H. and Choi, S. A. 2002. Cloning of the 5'-end and ampli-fication of full-length cDNA of gendipic RNA of LiIy symptomless virus. Ptant Pathol. J. 18:187-191 https://doi.org/10.5423/PPJ.2002.18.4.187
  16. Sanchez-Navarro, J. A. and Fallas, V. 1994. Nucleotide sequence of apple mosaic Ilarvirus RNA4. J. Gen. ViroI 75:1441-1445 https://doi.org/10.1099/0022-1317-75-6-1441
  17. Sanchez-Navarro, J. A. and Pallas, V. 1997. Evolutionary relation-ships in the IIarviruses: nucleotide sequence of prunusnecrotic ringspot virus RNA 3. Arch. ViroI. 142:749-763 https://doi.org/10.1007/s007050050116
  18. Scott, S. W. and Ge, X. 1995. The complete nucleotide sequenceof the RNA 3 of lilac ring mottle ilarvims. J. Gen. Virol.76:1801-1806 https://doi.org/10.1099/0022-1317-76-7-1801
  19. Shiel, P. J., Alrefai, R. H., Domier, L. L., K-orban, S. S. and Berger, P. H. 1995. The complete nucleotide sequence of applemosaic virus RNA-3. Anh. ViroI 140:1247-1256
  20. Shiel, P. J. and Berger, P. H. 2000. The complete nucleotidesequence of apple mosaic vIrus (ApMV) RNA 1 and RNA 2:ApMV is more closely related to alfalfa mosaic virus than to other Ilarviruses. J. Gen. ViroI. 81:273-278 https://doi.org/10.1099/0022-1317-81-1-273
  21. Van Regenmortel, M. H. V., Fauquet, C. M., Bishop, D. H. L., Carstens, E. B., Estes, M. K.., Lemon, S. M., Maniloff, J.,Mayo, M. A., McGeoch, D. J., Prihgle, C. R. and Wickner, R.B. 2000. Virus Taxonomy. Seventh Report of the International Committee on Taxonomy of Viruses. Academic Press, NY
  22. Vaskova, D., Petrzik, K. and Karesova, R. 2000. Variability and molecular typing of the woody-tree infecting prunus necrotic ringspot ilarvirus. Areh. Virol. 145:699-709 https://doi.org/10.1007/s007050050664
  23. Yoon, J. Y., Min, B. E., Choi, J. K. and Ryu, K. H. 2002. Genome structure and production of biologically active in vitro transcripts of cucurbit-infecting Zucchini green mottle mosaicvirus. Phytopathology 92:156-163 https://doi.org/10.1094/PHYTO.2002.92.2.156
  24. Zimmerman, M. T. and Scott, S. W. 2001 American plum line pattem virus is a distinct Ilarvirus. Acta Hort. 550:221-225

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  11. Molecular characterization of the Indian strain of Apple mosaic virus isolated from apple (Malus domestica) vol.37, pp.4, 2009, https://doi.org/10.1007/s12600-009-0041-8
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