Food Science of Animal Resources (한국축산식품학회지)

Korean Society for Food Science of Animal Resources (한국축산식품학회)
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Effects of Whey Protein Hydrolyzates Fractionated by Molecular Weight on the Growth of Bifidobacterium bifidum Bb-11

분자량에 따라 분획된 유청단백분해물이 Bifidobacterium bifidum Bb-11의 생장에 미치는 영향

  • 김완섭 (일본 북해도대학 낙농과학연구실) ;
  • 박승용 (연안축산원예대학 축산학부) ;
  • 이범진 (강원대학교 약학대학) ;
  • 김평현 (강원대학교 자연대학 미생물학과) ;
  • 고준수 (강원대학교 축산가공학과)
  • Published : 2002.03.01
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Abstract

This study was carried out to evaluate the effect of whey protein concentrate-80%(WPC-80) and whey protein isolate(WPI) on the growth of B. bifidum Bb-11. Whey proteins($\alpha$-lactalbumin, $\beta$-lactoglobulin) were digested with trypsin, then their hydrolyzates were separated into three fractions (>10,000Da, 3,000∼10,000Da, <3,000Da) by two-step ultrafiltration process with Centriprep 10 and Centricon-30. These three fractions by molecular weight were evaluate growth-promoting effects for the B. bifidum Bb-11. The results obtained were summarized as follows; The growth rate of B. bifidum Bb-11 tended to increase by supplementation of WPC-80 to basal medium, but decreased by supplementation of WPI. Two whey proteins were hydrolyzed by trypsin at 40$\^{C}$ for 6 hrs, and three fractions were collected by UF treatment and concentrated by Centricon-30. Collected concentrations of protein of F-I and F-II and F-III from $\alpha$-lactalbumin were 11.53mg, 7.79mg, and 5.21 mg and those of protein from $\beta$-lactoglobulin were 4.13mg, 5.30mg, and 9.351mg, respectively. Three fractions of $\alpha$-lactalbumin hydrolyzates promoted the growth rate of B. dbifidum Bb-11. Growth promoting activities of hydrolyzates(F-I and F-II) with molecular weight below 10,000Da were stronger than that of hydrolyzate(F-III) above 10,000Da. However, there was no significant difference between the hydrolyzate F-I and F-II. Three fractions of $\beta$-lactoglobulin hydrolyzates improves the growth rate of B.bifidum Bb-ll. The growth of B.bifidum Bb-ll was decreased after 24 hr incubation by supplementation of either F-II or F-III fraction compared to basal Whey medium, but maintained the enhancement by supplementation of F-I.

이 연구는 Whey배지에 Whey protein concentrate 80% (WPC-80)와 Whey protein isolate(WPI)를 첨가하여 B. bifidum Bb-11의 생장촉진효과를 알아보고자 실시하였다. 또 이 단백질들의 구성성분인 $\alpha$-Lactalbumin과 $\beta$-Lactogluburin을 trypsin으로 처리하여 얻어진 분해물을 분자량에 따라 분획하여 각 분획별로 그 효과를 구명하였으며, 그 결과를 요약하면 다음과 같다. WPC 첨가에 의한 효과는 대조구에 비하여 생장촉진효과가 있는 것으로 나타났으나, 첨가농도에 따른 차이는 근소하였다. WPI는 뚜렷한 생장촉진효과를 보여주지 못하였으며, 첨가량이 많을수록 생장이 억제되었다. 두 종류의 유청단백질을 4$0^{\circ}C$에서 6시간 동안 trypsin처리한 후 UF처리에 의하여 분획하고 각 분획들을 Centricon-30 으로 농축한 후, 얻어진 $\alpha$-lactalbumin 분해물 3분획에 대한 단백질농도는 각각 11.63mg, 7.79mg 및 5.21mg 이었고, $\beta$-Lactoglubulin 분해물 3분획은 각각 4.12mg, 5.30 mg 및 9.35mg 이었다. $\alpha$-Lactalbumin분해물 세 분획 모두 대조구에 비하여 높은 생장촉진효과를 나타내었으며, 그중에서 분자량 10,000~3,000Da(F-II)과 3,000Da 이하(F-III)의 두 분획이 모두 높게 나타난 것으로 보아, 분자량 10,000Da 이하의 peptide들이 bifidobacteria의 생장을 향상시키는 것으로 밝혀졌다. $\beta$-Lactoglubulin 분해물 세 분획들도 대조구에 비하여 생장이 좋게 나타났다. 분자량 10,000Da 이하의 peptide를 함유하는 두 분획은 생장이 우수하였으며 배양 24 시간후에는 분자량 3,000Da 이상의 두 분획(F-I, F-II)이 대조구에 비하여 빠르게 생장이 저하된 반면, 분자량 3,000 Da이하의 분획(F-III)에서는 계속적인 생장촉진효과를 보여주었다.

Keywords

References

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