Biotechnology and Bioprocess Engineering:BBE

  • 제7권3호
  • /
  • Pages.121-129
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  • 2002
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  • 1226-8372(pISSN)
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  • 1976-3816(eISSN)
한국생물공학회 (The Korean Society for Biotechnology and Bioengineering)
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Molecular Breeding of Genes, Pathways and Genomes by DNA Shuffing

  • Stemmer, Willem P.C. (Maxygen, Inc., 515 Galveston Drive, Redwood City)
  • 발행 : 2002.05.01
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초록

Existing methods for optimization of sequences by random mutagenesis generate libraries with a small number of mostly deleterious mutations, resulting in libraries containing a large fraction of non-functional clones that explore only a small part of sequence space. Large numbers of clones need to be screened to find the rare mutants with improvements. Library display formats are useful to screen very large libraries but impose screening limitations that limit the value of this approach for most commercial applications. By contrast, in both classical breeding and in DNA shuffling, natural diversity is permutated by homologous recombination, generating libraries of very high quality, from which improved clones can be identified with a small number of complex screens. Given that this small number of screens can be performed under the conditions of actual use of the product, commercially relevant improvements can be reliably obtained.

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참고문헌

  1. Bio/Technology v.13 Searching sequence space Stemmer, W. P. C. https://doi.org/10.1038/nbt0695-549
  2. Proc. Natl. Acad. Sci. v.91 DNA shuffling by random fragmentation and reassembly: In vitro recombination for molecular evolution Stemmer, W. P. C https://doi.org/10.1073/pnas.91.22.10747
  3. Nature v.370 Rapid evolution of a protein invitro by DNA shuffling Stemmer, W. P. C https://doi.org/10.1038/370389a0
  4. Nature Biotechnol v.14 Improved green fluorescent protein by molecular evolution using DNA shuffling Crameri, A.;E. Whitehorn;E. Tate;W. P. C. Stemmer https://doi.org/10.1038/nbt0396-315
  5. Proc. Natl. Acad. Sci. v.94 Evolution of an effective fucosidase from a galactosidase by DNA shuffling and screening Zhang, J.;G. Dawes;W. P. C. Stemmer https://doi.org/10.1073/pnas.94.9.4504
  6. Nature Biotechnol. v.15 Molecular evolution of an arsenate detoxification pathway by DNA shuffling Crameri, A.;G. Cawes;E. Rodriguez;S. Silver;W. P. C. Stemmer https://doi.org/10.1038/nbt0597-436
  7. Nature v.391 DNA shuffling of genes from diverse species accelerates directed evolution Crameri, A.;S.-A. Raillard;E. Bermudez;W. P. C. Stemmer https://doi.org/10.1038/34663
  8. Gene v.164 Single-step PCR assembly of a gene and a whole palsmid from large numbers of oligonucleotides Stemmer, W.P.C.;A. Crameri;K. D. Ha;T. M. Brennan;H. L. Heyneker https://doi.org/10.1016/0378-1119(95)00511-4
  9. Nature Biotechnol v.17 Directed evolution of thymidine kinase for AZT phosphorylation using DNA family shuffling Christians, R. C.;L. Scapozza;A. Crameri;G. Folkers;W. P. C. Stemmer https://doi.org/10.1038/7003
  10. Nature Biotechnol v.17 Creation of a functionally diverse enzyme library by DNA family shuffling Ness, J.;M. Welch, L.;Giver, M. Bueno;J. Cherry;T. Borchert;W. P. C. Stemmer;J. Minshull https://doi.org/10.1038/12884
  11. Nature Biotechnol v.17 Rapid evolution of a cytokine using molecular breeding Chang, C.-C.;T. T. Chen;B. W. Cox;G. N. Dawes;W. P. C. Stemmer;J. Punnonen;P. A. Patten https://doi.org/10.1038/11737
  12. Nature Genetics v.25 Molecular breeding of viruses Soong, N.-W.;L. Nomura;K. Pekrun;M. Reed;L. Sheppard;G. Dawes;W. P. C. Stemmer https://doi.org/10.1038/78132
  13. Nature Biotechnol v.18 Breeding of retroviruses by DNA shuffling for improved stability and processing yields Powell, S. K.;M. A. Kaloss;A. Pinkstaff;R. McKee;I. Burimski;M. Pensiero;E. Otto;W. P.C. Stemmer;N.-W. Soong https://doi.org/10.1038/82391
  14. Tumor Targeting v.4 Molecular breeding of viruses for targeting and other clinical properties Stemmer, W. P. C.;N.-W. Soong
  15. Nature v.415 Genome shuffling leads to rapid phenotypic improvement in bacteria Zhang, Y.-X.;K. Perry;V. A. Vinci;K. Powell;W. P. C. Stemmer;S. B. del Cardayre https://doi.org/10.1038/415644a
  16. Chemistry Biol. v.125 Novel enzyme activities and functional plasticity revealed by recombining highly homologous enzymes Raillard, S.;A. Krebber;Y. Chen;J. E. Ness;E. Bermudez;R. Trinidad;R. Fullem;C. Davis;M. Welch;J.Seffernick;L. P. Wackett;W. P. C. Stemmer;J. Minshull