Biotechnology and Bioprocess Engineering:BBE

  • 제7권2호
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  • Pages.95-99
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  • 2002
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  • 1226-8372(pISSN)
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  • 1976-3816(eISSN)
한국생물공학회 (The Korean Society for Biotechnology and Bioengineering)
권호 표지

Cloning and Characterization of Mannheimia succiniciproducens MBEL55E Phosphoenolpyruvate Carboxykinase (pckA) Gene

  • Lee, Sang-Yup (Metabolic and Biomolecular Engineering, National Research Laboratory, Department of Chemical & Biomolecular Engineering and BioProcess Engineering, Research Center, Korea Advanced Institute of Science and Technology) ;
  • Lee, Pyung-Cheon (Metabolic and Biomolecular Engineering, National Research Laboratory, Department of Chemical & Biomolecular Engineering and BioProcess Engineering, Research Center, Korea Advanced Institute of Science and Technology) ;
  • Hong, Soon-Ho (Metabolic and Biomolecular Engineering National Research Laboratory) ;
  • Chang, Ho-Nam (Department of Chemical & Biomoleculr Engineering and BioProcess Engineering Research Center, Korea Advanced Institute of Science and Technology)
  • 발행 : 2002.03.01
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초록

A pckA gene encoding phosphoenolpyruvate carboxykinase (PEPCK) was cloned and sequenced from the succinic acid producing bacterium Mannheimia succiniciproducens MBEL55E. The gene encoded a 538 residue polypeptide with a calculated molecular mass of 58.8 kDa and a calculated pI of 5.03. The deduced amino acid sequence of the M. succiniciprodutens MBEL55E PEPCK was similar to those of all known ATP-dependent PEPCKS.

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참고문헌

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