Journal of Chest Surgery

The Korean Society for Thoracic and Cardiovascular Surgery (대한심장혈관흉부외과학회)
권호 표지

The Effects of Muscle Cell Transplantation into the Hearts of the Hamsters with a Dilated Cardiomyopathy

배양한 근육세포를 확장성 심근증을 가진 햄스터 심장에 이식 후 심장기능의 변화연구

  • 유경종 (연세대학교 의과대학 심장혈관병원 심장혈관외과) ;
  • 임상현 (연세대학교 의과대학 심장혈관병원 심장혈관외과) ;
  • 송석원 (연세대학교 의과대학 심장혈관병원 심장혈관외과) ;
  • 홍유선 (연세대학교 의과대학 심장혈관병원 심장혈관외과) ;
  • 박현영 (연세대학교 의과대학 심장혈관병원 심혈관연구소)
  • Published : 2002.05.01
Download PDF
⟨ Previous Next ⟩

Abstract

Background: Recently, cell transplantation has been extensively investigated to improve heart function in dysfunctional heart. This study was designed to compare the effects of smooth muscle cells (SMC) and heart cells (HC) transplantation in dilated cardiomyopathic hamsters. Material and Method: HC and SMC were isolated from heart and ductus deferens of BIO 53.58 hamsters, and cultured for transplantation. HC and SMC or culture medium were transplanted into the left ventricle of 17 weeks old adult hamsters in HC transplanted (HCTx), SMC transplantation (SMCTX), and control groups (Con) (N = 10 each). Cyclosporine (5 mg/Kg) was administered subcutaneously for HCTx. Sham operated hamsters (N=10) underwent the surgery but did not receive an injection. At 4 weeks after transplantation, heart function was evaluated in all groups using a Langendorff perfusion apparatus. Result: Histology showed severe focal myocardial necrosis in all groups. HCTx and SMCTx formed huge muscle tissue in dilated myocardium. SMCTx and HCTx had better heart function than Con and sham (p<0.01). And SMCTx had better peak systolic pressure (p<0.05) antral developed pressure (p<0.05) than HCTx. But sham and Con did not any statistical make difference. Conclusion: SMCTx and HCTx formed muscle tissue and improved ventricular function in hamsters with dilated cardiomyopathy And SMCTx showed better heart function in peak systolic pressure and developed pressure than HCTx.

배경: 최근 들어 심부전증을 가진 심장에 세포이식을 이용하여 심장기능을 개선시키고자 하는 연구가 활발히 진행되고 있다. 이 연구는 확장성 심근증을 가진 햄스터 심장에 배양한 평활근 세포와 심근세포를 이식한 후 심장기능의 변화를 관찰하였다. 대상 및 방법: 심근세포와 평활근세포는 BIO 53.58 햄스터의 심장과 옃션 deferens에서 분리하여 배양하였다. 실험군은 각각 10마리로서 심근세포 (1군)와 평활근세포 (2군) 및 배양액 (3군)을 17주된 BIO 53.58 햄스터의 좌심실에 이식하였고 Cyclosporine (5mg/Kg)을 1군에 한하여 수술 직후부터 매일 피하주사하였다. sham군 (4군) 은 세포나 배양액의 이식 없이 단순 흉부수술만을 시행하였다. 세포나 배양액의 이식 4주 후에 Langendorff 체외순환 모델을 이용하여 좌심실기능을 측정하였다. 결과: 조직학적 검사상 모든 군에서 심한 심근괴사가 있었고, 1군과 2군에서는 수여심장의 심근 내에서 새로운 근육조직이 형성되었다. 좌심실기능의 평가에서 1군과 2군은 3군과 4군에 비해 통계적으로 유의하게 우수하였고 (p<0.01), 2군은 1군에 비해 수축기 좌심실압과 발생기압이 통계적으로 유의하게 우수하였다 (p<0.05). 그러나 3군과 4군 사이에는 통계적인 유의성이 없었다. 절론 확장성 심근증을 가진 햄스터 심장에 배양한 평활근 세포와 심근세포를 이식한 결과 수여심장의 심근 내에서 근육조직을 형성하고 좌심실기능을 개선시켰으며, 이 중 평활근세포를 이식한 심장이 수축기 좌심실압과 발생기압이 더 우수한 좌심실기능 개선효과를 보여주었다.

Keywords

References

  1. Circulation v.93 Report of the 1995 World Health Organization/International Society and Federation of Cardiology Task Force on the definition and classification of cardiomyopathies Richardson P;McKenna W;Bristow M(et al.) https://doi.org/10.1161/01.CIR.93.5.841
  2. J.Heart Lung Transplant v.18 no.7 The registry of the International Society for Heart and Lung Transplantation: Sixteenth official report-1999 Hosenpud JD;Bennett LE;Keck BM;Fiol B;Boucek MM;Novick RJ https://doi.org/10.1016/S1053-2498(99)00037-6
  3. Ann Thorac Surg v.68 Permanent ventricular assist device support versus cardiac transplantation Pennington DG;Oaks TE;Lohmann DP https://doi.org/10.1016/S0003-4975(99)00623-2
  4. J Heart Lung Transplant v.17 no.4 Morbidity,functional status,and immunosuppressive therapy after heart transplantation: An analysis of the Joint International Society for Heart and Lung Transplantation / United Network for Organ Sharing Thoracic Registry Brann WM;Bennett LE;Keck BM;Hosenpud JD
  5. Ann Thorac Surg v.62 Cardiomyocyte transplantation improves heart function Li R-K;Jia ZQ;Weisel RD(et al.) https://doi.org/10.1016/S0003-4975(96)00389-X
  6. Circulation v.96 no.suppl.Ⅱ Natural history of fetal rat cardiomyocytes transplanted into adult rat myocardial scar tissue Li R-K;Mickle DAG;Weisel RD(et al.)
  7. Cell Transplantation v.17 Cardiomyocyte transplantation in a porcine myocardial infarction model Watanabe E;Smith DM;Delcarpio JB(et al.)
  8. Nature Medicine v.4 Regenerating functional myocardium; Improved performance after skeletal myoblast transplantation Taylor DA;Atkins BZ;Hungspreugs P(et al.) https://doi.org/10.1038/nm0898-929
  9. Ann Thorac Surg v.60 Cellular cardiomyoplasty: Myocardial regeneration with satellite cell implantation Chiu RC-J;Zibaitis A;Kao RL https://doi.org/10.1016/S0003-4975(95)00374-6
  10. J.Mol Cell Cardiol v.31 Smooth muscle cell transplantation into myocardial scar tissue improves heart function Li R-K;Jia ZQ;Weisel RD;Merante F;Mickle DAG https://doi.org/10.1006/jmcc.1998.0882
  11. Circulation v.102 Heart Cell Transplantation Improves Heart Function in Dilated Cardiomyopathic Hamsters Yoo K-J;Li R-K;Weisel RD(et al.)
  12. Ann Thorac Surg v.70 Autologous Smooth Muscle Cell Transplantation Improved Heart Function in Dilated Cardiomyopathy Yoo K-J;Li R-K;Weisel RD;Mickle DAG;Li GM;Yau TM(ed.) https://doi.org/10.1016/S0003-4975(00)01630-1
  13. Circulation v.98 no.19 suppl. Can cellular transplantation improve function in doxorubicin-induced heart failure? Scorsin M;Hagege AA;Dolizy I(et al.)
  14. J.Tiss Cult Meth v.14 Method of culturing cardiomyocytes from human pediatric ventricular myocardium Li RK;Weisel RD;Williams WG;Mickle DAG https://doi.org/10.1007/BF01404750
  15. J.Mol Cell Cardiol v.28 Depressed transient outward current density in ventricular myocytes from cardiomyopathic Syrian hamsters of different ages Thuringer D;Coulombe A;Deroubaix E;Coraboeuf E;Mercadier J-J https://doi.org/10.1006/jmcc.1996.0036
  16. Mol Cell Biochem v.156 Alteration of extracellular matrix in dilated cardiomyopathic hamster heart Okada H;Kawaguchi H;Kudo T(et al.)
  17. JPET v.283 Abnormalities in plasma catecholamines and myocardial adrenoreceptors in cardiomyopathic BIO 53.58 Syrian hamsters and improvement by metoprolol treatment Yamada S;Ohkura T;Yamadera T(et al.)
  18. Br J.Pharmacol v.123 Effect of trimetazidine and verapamil on the cardiomyopathic hamster myosin phenotype DHahan N;Taouil K;Janmot C;Morel JE https://doi.org/10.1038/sj.bjp.0701643
  19. Eur J Cardiothorac Surg v.13 Partial left ventriculectomy and mitral valve repair for end-stage heart failure McCarthy JF;McCarthy PM;Starling RC(et al.) https://doi.org/10.1016/S1010-7940(98)00013-X
  20. Ann Thorac Surg v.68 no.2 Dynamic cardiomyoplasty Acker MA https://doi.org/10.1016/S0003-4975(99)00454-3