KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Solubillzation and Extraction Of Antioxidant Astaxanthin by Micelle Formation from Phaffia rhodozyma Cell Homogenate

Phaffia rhodozyma 세포파쇄액으로부터 항산화제 Astaxanthin의 미셀 형성을 통한 가용화 및 추출

  • Kim, Young-Beom (Process Engineering Team, Doosan Biotech) ;
  • Ryu, Kang (Bioprocessing Research Laboratory, Department of Chemical Engineering, Hanyang University) ;
  • Lim, Gio-Bin (Department of Chemical Engineering, The University of Suwon) ;
  • Lee, Eun-Kyu (Bioprocessing Research Laboratory, Department of Chemical Engineering, Hanyang University)
  • 김영범 ((주)두산바이오텍) ;
  • 류강 (한양대학교 화학공학과 생물공정연구실) ;
  • 임교빈 (수원대학교 화학공학과) ;
  • 이은규 (수원대학교 화학공학과)
  • Published : 2002.04.01
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Abstract

Astaxanthin (3,3＇-dihydroxy-${\beta}$, ${\beta}$-carotene-4-4＇-dione), a natural pigment of pink to red color, is widely distributed in nature particularly in the skin layer of salmonoids and the crust of shrimp, lobster, etc. Recently, it was produced from the yeast culture of Phaffia rhodozyma. Because of its high thermal stability and antioxidant functionality, its applications can be extended into food, cosmetics, and pharmaceutical ingredient beyond the traditional feed additive. Because of its very high lipophilicity, astaxanthin has been extracted traditionally by strong organic solvents such as chloroform, petroleum ether, acetone, etc. In this study, we developed a surfactant-based solubillization system for astaxanthin, and used it to extract astaxanthin from disrupted yeast cells. Among Tween 20, Triton X-100 and SDS, Tween 20 was identified as the most suitable surfactant in terms of extraction capacity and safety. The ethylene oxide group of Tween 20 was identified as the most significant factor to increase the HLB value that determined the extraction capacity. The effects of micelle formation condition, such as the molar ratio of astaxanthin and Tween 20, pH, and ionic strength were also investigated. pH and ionic strength showed no significant effects. The optimal molar ratio between astaxanthin and Tween 20 was 1 : 12. Antioxidant activity of astaxanthin was higher than ${\beta}$-carotene and ${\alpha}$-tocopherol. Astaxanthin in the crude extract from the yeast cell was more resistant to air and/or light degradation than pure astaxanthin, probably because of the presence of other carotenoids and lipids.

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References

  1. Pure & Appl. Chem. v.63 Biological function and activities of animal carotenoid Wataru, M. https://doi.org/10.1351/pac199163010141
  2. Lipids v.24 no.7 Antioxidant Activity of β-Carotene-Related Carotenoids in Solution Tera, J. https://doi.org/10.1007/BF02535085
  3. Cartenoids and the Immune system in carotenoids Bendich, A.
  4. Phytochemistry v.15 Carotenoids of Paffa rhodozyma, a red pigmented fermenting yeast Andrewes, A. G.;H. J. Phaff;M. P. Starr https://doi.org/10.1016/S0031-9422(00)84390-3
  5. Oxidative stress: oxidants and antioxidants Sies, H.
  6. Kor. J. Biotechnol. Bioeng. v.15 no.2 Fermentation Process characteristics of Phaffia rhodazyma Mutant B76 for Astaxanthin Biosynthesis Lim, D. T.;E. K. Lee
  7. J. Appl. Bacteriol. v.70 Microbial sources of Carotenoid pigments used in foods and feeds Nelis, H. J.;A. P. De Leenheer https://doi.org/10.1111/j.1365-2672.1991.tb02922.x
  8. Nature v.175 Carotenogensis in Haematococcus pluvialis Droop, M. R.
  9. J. Ferment. Bioeng. v.77 Isolation of Resistant Mutants against Carotenoid Biosynthesis Inhibitors for a Green Algae Protoplast Fusion for Breeding of higher Astaxanthin Producers Tjahjono, A. E.;T. Kakizono;Y. Hayama;N. Nishio;S. Nagai https://doi.org/10.1016/0922-338X(94)90003-5
  10. J. Ferment. Bioeng. v.82 Autotrophic Growth and Carotenoid Production of Haematococcus pluvialis in a 30 Liter Air-Lift Photobioreactor Harker, M.;A. J. Tsavalos;A. J. Young https://doi.org/10.1016/0922-338X(96)85031-8
  11. Biotech. Letters v.10 no.9 Astaxanthin formation by the yeast phaffia rhodozyma on molasses Hard, N. F. https://doi.org/10.1007/BF01024710
  12. J. Agric. Food Chem. v.43 Determination of Optimun Conditions for Supercritical Fluid Extraction of Carotenoids from Carrot(Daucus carota L.) Tissue Barth, M. M.;C. Zhou;K. M. Kute;G. A. Rosenthal https://doi.org/10.1021/jf00059a019
  13. Pharm. Technol. v.March Using micromulsions for drug delivery Bhargava, H. N.;Narurkar, A.;Lieb, L. M.
  14. Chemistry and Biochemistry of Plant Pigment v.2 Carotenoids Davies, B. H.;T. W. Goodwin(ed)
  15. Kor. J. Bot. v.33 no.1 Effects of Dimethin on the Senescence of Excised Barley First Leaves Jun, B. O.
  16. Method of Enzymatic Analysis v.4 Inorginic peroxidases Bernt, E.;H. U. Bergmeyer;H. U. Bergmeyer(ed)
  17. Chem. Eng. v.49 Trans. Industn. Hetherington, P. J.;Fellow, M.;Dunnill, P.;Lilly, M. D.
  18. J. Kor. Ind. & Eng. Chemistry v.9 no.7 Effects of Salts and Isomeric Butanols on the Mixed Micellar Properties of Cetylpyridinium Chloride with Triton X-100 Chung, J. J.;S. H. Lee;Y. C. Kim;B. H. Lee
  19. Appl. Microbiol. Biotechnol. v.48 Antioxidant role of astaxanthin in the green algae Haematococcus pluvialis Kobayashi, M.;T. Kakizono;N. Nishio;S. Nagai;Y. Kurimura;Y. Tsuji https://doi.org/10.1007/s002530051061