Prevalence of Staphylococcus aureus in Kimbap

김밥 중 황색포도상구균의 분포조사

  • 김창민 (식품의약품안전청 식품미생물과) ;
  • 강윤숙 (식품의약품안전청 식품미생물과) ;
  • 윤선경 (식품의약품안전청 식품미생물과) ;
  • 좌승협 (식품의약품안전청 식품미생물과) ;
  • 이동하 (식품의약품안전청 식품미생물과) ;
  • 우건조 (식품의약품안전청 식품미생물과) ;
  • 박영식 (한국보건산업진흥원)
  • Published : 2002.03.01

Abstract

According to food poisoning statistic data of 2000, the food poisoning outbreaks have occurred mainly by meat (27.9%), shellfish and its processed products (26.0%), and ready-to eat meals (24.0%) such as Kimbap and packed lunch boxes. The major causative flood poisoning bacteria were Salmonella spp. (35.6%), Staphylococcus aureus(11.3%) and Vibrio parahaemolyticus (3.2%). In this study, we conducted the isolation and enumeration for S. aureus in Kimbap. This monitoring data will be applied to the following study, risk assessment. The Kimbap samples were collected from department stores, convenient stores and snack bars located in Seoul, Busan, Daejeon, and Gwangju. The overall isolation rate of S. aureus from 214 Kimbap samples was 34.1% and the average count was 623 cells. Enterotoxin typing test for isolates showed 42.5%, 4.1% and 2.7% for type, A, B and C, respectively. There was no significant seasonal difference in S. aureus isolation, but the average count in summer(793 cfu/g) was 1.8 times higher than that of winter(446 cfu/g).

2000년 국내 식중독발생 통계에 따르면, 원인식품별 식중독발생건수는 육류 등이 27.9%, 어패류 및 가공품이 26.0%, 복합조리식품(김밥, 도시락)이 24.0%의 비율을 차지하고 있으며, 원인균별 식중독발생건수는 Salmonella spp.가 28.8%로 1위, Vibrio parahaemolyticus가 13.5%로 2위, Staphylococcs aureus가 8.7%로 3위를 차지하고 있다. 이에 본 연구에서는 김밥 중 황색포도상구균에 대한 분포도조사 및 오염정도를 분석하여 위해도평가의 기초자료로 활용하고자 하였다. 서울, 부산, 대전, 광주의 백화점, 편의점, 분식점 등에서 구입한 김밥 총 214건에 대한 황색포도상구균의 정량 및 정성실험 결과, 균검출율은 34.1%였고, 평균균량은 623 cfu/g 이었다. 분리균에 대한 enterotoxin 실험결과 A type 42.5%(31주/73주), B type 4.1%(3주/73주), D type 2.7%(2주/73주)의 분포를 보였다. 또한 유통·판매형태별로는 분식점의 검출율이 42.8%로 백화점(25.8%)및 편의점(12.3%)보다 높게 나타났으며, 계절별 평균균검출율은 비슷하였으나, 정량검사결과 여름철(793 cfu/g)에 겨울철(446 cfu/g)보다 균량이 1.8배 높게 나타났다.

Keywords

References

  1. Kim, D.H., Kwon, K.R., Lee, K.H., Ju, Y.R., Oh, K.S. and Kark, H.S.: Studies on Staphylococcal food-poisoning and enterotoxin productivity, Report of National Institute of Health, 26, 111-121 (1989)
  2. Ryu, P.Y, Kim, Y.I., Rhee, l.H., Chung, S.S., Ahn, T.R., Shin, lH., Ryang, D.W., Kim, YH.: Antimicrobial resistance and plasmid profile of methicillin-resistant S. aureus, J. Infection, 27(1), 15-29 (1995)
  3. Yi, D.H. and Hong, H.S.: Isolation and cultural conditions of Actinornycetes strain producing effective antibiotic for MRSA, 건국기술연구논문집, 24 (1999)
  4. Jung, C.H. and Kang, H.J.: Antimicrobial susceptibilities and-lactamase production of S. aureus isolated from bulk milk and domestic animals, Kor. J. Vet. Publ. Hlth., 17(3) (1993)
  5. 김승곤, 김충환, 김태운, 이건섭, 정격석: 최신병원위생학, 고문사, pp.251-256 (2000)
  6. Kim, D.H., Kim, B.S., Lee, K.H., Ju, Y.R., Oh, K.S., Kark, H.S. and Choi. Y.K.: A study on diagnosis of Staphylococcal food poisoning and enterotoxin, The Report of National Institute of Health, 27(1), 56-63 (1990)
  7. Lee, K.U., Kim, Y.J., Lee, S.M., Lee, M.S. and Kim, R.H.: Nonstaining method for determination of gram reactions and comparision of the API Staph(DMS) and Cowan and Steel procedures for the identification of Staphylococcus and Micrococcus, Report of NIH Korea, 25, 277-288 (1988)
  8. Lee, K.U., Kim, Y.J., Lee, S.M., Lee, M.S. and Kim, R.H.: Nonstaining method for determination of gram reactions and comparision of the API Staph(DMS) and Cowan and Steel procedures for the identification of Staphylococcus and Micrococcus, Report of NIH Korea, 25, 277-288 (1988)
  9. Cho, D.T.: Significance of methicillin-resistant S. aureus as a nosocomial pathogen, J. Infection, 27(1), 11-13 (1995) https://doi.org/10.1016/0163-4453(93)93488-P
  10. 정갑수: S.aureus의 增殖과 enterotoxin產生에 미치는 pH, 溫度 및 食品添加物 의 影饗. 전남대학교 박사학위논문 (1992)
  11. Kim, D.H., Kwon, K.R., Lee, K.H., Ju, Y.R., Oh, K.S. and Kark, H.S.: Study on Staphylococcal enterotoxin, Report of NIH Korea, 25, 297-307 (1988)
  12. Atanassova, V, Meindl, A., Ring, C.: Prevalence of Staphylo-coccus aureus and staphylococcal enterotoxins in raw pork and uncooked smoked ham-a comparison of classical culturing detection and RFLP-PCR, Int. J. Food Microbiol., 68, 105-113 (2001) https://doi.org/10.1016/S0168-1605(01)00479-2
  13. Shin, H.K., Kim, J.B., Lee, J.H., Kim, Y.K. and Kim, C.K.: Development of the simple immunoassays for the measurement of Staphylococcal enterotoxins, J. Anim. Sci. Tech., (1993)
  14. 장동석, 신동화, 정덕화, 김창민, 이인선: 식품위생학, 정문각, pp.45-133 (1999)
  15. Chen, T.R., Hsiao, M.H., Chiou, C.S., Tsen, H.Y.: Development and use of PCR primers for the investigation of C1, C2 and C3 enterotoxin types of Staphylococcus aureus strains isolated from food-borne outbreaks, Int. J Food Microbial., 71, 63-70 (2001) https://doi.org/10.1016/S0168-1605(01)00564-5
  16. Hepner, E.: Food poisoning and Salmonella infections in England and Wales, 1976-1978, Publ. Hlth. Land., 94, 337-349 (1980) https://doi.org/10.1016/S0033-3506(80)80136-3
  17. Noterman, S. and Heuvelman, C.J. : Combined effect of water activity, pH and sub-optimal temperature on growth and enterotoxin production of S. aureus, J Food Sci., 48, 1832-1840 (1983) https://doi.org/10.1111/j.1365-2621.1983.tb05096.x
  18. Vernon, E. and Tillett, H.E.: Food poisoning and Salmonella infections in England and Wales, 1969-1972, Publ. Hlth. Lond., 88, 225-235 (1974) https://doi.org/10.1016/S0033-3506(74)80049-1
  19. Casman, E.P., Bennett, R.W., Dorsey, A.E., and Issa, l.A.: Identification of a. fourth Staphylococcal enterotoxin, enterotoxin D, J Bacteriol., 94, 1875-1882 (1967)
  20. 李恩重: The Effect of temperature and time on the multiplication of Staphylococcus in foods, Korean Journal of Public Health, 9(2), 381-387 (1972)
  21. Brown, M.H.: Meat microbiology, Applied Science Publishers. London and New York, pp.269-486 (1982)
  22. Bacteriological Analytical Manual, Chap. 12, Food and Drug Administration, 8th Ed. (1995)
  23. 식품공전(별책), 식품의약품안전청, pp. 107 (2000)
  24. Norinaga, M., Asako, K., Takashi, M., Masato, A.: An outbreak of food poisoning due to egg yolk reaction-nagative Staphylococcus aureus. Int. J Food Microbial, 64, 361-366 (2001) https://doi.org/10.1016/S0168-1605(00)00446-3
  25. Monday, S. R. and Bohach, G.A. : Use of multiplex PCR to detect classical newly described pyrogenic toxin genes in staphylococcal isolates, J. Clin. Microbial., 37, 3411-3414 (1999)