Preventive Nutrition and Food Science

  • 제7권1호
  • /
  • Pages.18-21
  • /
  • 2002
  • /
  • 2287-1098(pISSN)
  • /
  • 2287-8602(eISSN)
한국식품영양과학회 (The Korean Society of Food Science and Nutrition)
권호 표지
DOI QR코드

DOI QR Code

Isolation of Xanthine Oxidase Inhibitors from Ginkgo biloba Leaves-Derived Components

  • Baek, Bong-Rae (Research Center for Industrial Development of Biofood Materials and Institute of Agricultural & Technology Chonbuk National University) ;
  • Kim, Moo-Key (Research Center for Industrial Development of Biofood Materials and Institute of Agricultural & Technology Chonbuk National University) ;
  • Lee, Sung-Eun (Research Center for Industrial Development of Biofood Materials and Institute of Agricultural & Technology Chonbuk National University) ;
  • Hwang, Young-Hee (Research Center for Industrial Development of Biofood Materials and Institute of Agricultural & Technology Chonbuk National University) ;
  • Lee, Hoi-Seon (Research Center for Industrial Development of Biofood Materials and Institute of Agricultural & Technology Chonbuk National University)
  • 발행 : 2002.03.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

The extract of Ginkgo biloba leaves was measured for inhibitory activity against xanthine oxidase. Aceton extract of G. biloba leaves showed strong inhibitory activity. Inhibitory activities of the fractionated extract were in the order of water > ethyl acetate fractions. Two fractions exhibiting strong inhibitory activities ware further purified via repeated silica gel, Amberlite IRN-78, Polyclar AT, and Sephadex LH-20 column chromatographies. Active components were isolated and identified through $^1$H-NMR and $^{13}$ C-NMR. The compounds were characterized as kaempferol 3-Ο-$\alpha$-(6$^{"′}$-p-coumaroylglucosyl-$\beta$-1,4-rhamnoside), and quercertin 3-Ο-$\alpha$-(6$^{′}$-p-coumaroylglucosyl-$\beta$-1,4-rhamnoside).

키워드

참고문헌

  1. Li H, Meng JC, Cheng CHK, Higa T, Tanaka J, Tan RX. 1999. New guaianolides and xanthine oxidase inhibitory flavonols from Ajania fruticulosa. J Nat Prod 62: 1053-1055. https://doi.org/10.1021/np990009i
  2. Izumida H, Adachi K, Mihara A, Yasuzawa T, Sano H. 1997. Hydroxyakalone, a novel xanthine oxidase produced by a marine bacterium, Agrobacterium aurantiacum. J Antibiotics 50: 916-918. https://doi.org/10.7164/antibiotics.50.916
  3. Halliwell B, Gutteridge JMC, Cross CE. 1992. Free radicals, antioxidants and human disease: Where are we now? J Lab Clin Med 119: 598-620.
  4. Larson RA. 1997. Naturally Occurring Antioxidants. Larson RA, ed. Lewis Publishers, USA.
  5. Noro T, Noro K, Miyase T, Kuroyanagi M, Umehara K, Ueno A, Fukushima S. 1987. Inhibition of xanthine oxidase by anthraquinones. Chem Pharm Bull 35: 4314-4316. https://doi.org/10.1248/cpb.35.4314
  6. Noro T, Oda Y, Miyase T, Ueno A, Fukushima S. 1983. Inhibitors of xanthine oxidase from the flowers and buds of Dephne Genkwa Chem Pharm Bull 31: 3984-3987. https://doi.org/10.1248/cpb.31.3984
  7. Noro T, Ueno A, Mizutani M, Hashimoto T, Miyase T, Kuroyanagi M, Umehara K, Fukushima S. 1984. Inhibitors of xanthine oxidase from the flowers and buds of Athyrium mesosorum. Chem Pharm Bull 32: 4455-4459. https://doi.org/10.1248/cpb.32.4455
  8. Nakanishi T, Nishi M, Inada A, Obata H, Tanabe N, Abe S, Wakashiro M. 1990. Two new potent inhibitors of xanthine oxidase from leaves of perilla frutescens britton var. acuta kudo. Chem Pharm Bull 38: 1772-1774. https://doi.org/10.1248/cpb.38.1772
  9. Haraguchi H, Saito T, Okamura N, Yagi A. 1995. Inhibition of lipid peroxidation and superoxide generation by diterpenoids from Rosmarinus officinalis. Planta Med 61: 333-336. https://doi.org/10.1055/s-2006-958094
  10. Potterat O. 1997. Antioxidants and free radical scavengers of natural origin. Current Organ Chem 1: 415-440.
  11. Choi U, Shin DH, Chang YS, Shin JI. 1992. Screening of natural antioxidant from plant and their antioxidative effect. Korean J Food Sci Technol 24: 142-148.
  12. Kim HK, Kim YE, Do JR, Lee YC, Lee BY. 1995. Antioxidantive activity and physiological activity of some Korean medicinal plants. Korean J Food Sci Technol 27: 80-85.
  13. Yen GC, Chen HY. 1995. Antioxidant activity of various tea extracts in relation to their antimutagenicity. J Agric Food Chem 43: 27-32. https://doi.org/10.1021/jf00049a007
  14. Halliwell B, Gutteridge JMC. 1990. Role of free radicals and catalytic metal ions in human disease. Meth Enzymol 186: 1-85. https://doi.org/10.1016/0076-6879(90)86093-B
  15. Hasegawa R, Chujo T, Sai-kato K, Uemura T, Tanimura A, Kurokawa Y. 1995. Preventive effects of green tea against liver oxidative DNA damage and hepatotoxicity in rats treated with 2-nitropropane. Food Chem Toxicol 33: 961-970. https://doi.org/10.1016/0278-6915(95)00064-9