Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Identification of Biologically Active Compounds from Panax ginseng C. A. Meyer

한국산 인삼의 polyphenol 화합물의 생리활성 효과

  • Choi, Hee-Jin (Department of Food Science and Technology Yeungnam University) ;
  • Zhang, Yun-Bin (Department of Food Science and Technology Yeungnam University) ;
  • An, Bong-Jeon (Faculty of Life Resources and Engineering, Kyungsan University) ;
  • Choi, Cheong (Department of Food Science and Technology Yeungnam University)
  • 최희진 (영남대학교 식품가공학과) ;
  • 장운빈 (영남대학교 식품가공학과) ;
  • 안봉전 (경산대학교 생명자원과학부) ;
  • 최청 (영남대학교 식품가공학과)
  • Published : 2002.06.01
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Abstract

The polyphenol compounds of Korea ginseng radix were extracted with 60% acetone for 4 days at room temperature and purified using Sephadex LH-20 column chromatography, MCI gel column chromatography, Bondapak $C_{18}$, column chromatography, TLC and HPLC. As a result in three compounds were isolated from Korean ginseng. In the inhibitory activities of angiotensin converting enzyme, compound Ⅱ showed the highest value of 31.86% inhibition at 157 ppm. Compound I showed 19.4% inhibition at 157 ppm. In the inhibitory activities of xanthine oxidase, compound I, II showed complete inhibition at 666 ppm but compound III didn't have inhibitory activity. In the inhibitory activities of tyrosninase, compound III showed 6.1% inhibition at 300 ppm and 28.6% at 400 ppm.

한국산 인삼 5년근을 60% acetone으로 추출하여 Sepadex LH-20 gel column chromatography, MCI-CHP 20 gel column chromatography, Bondapak $C_{18}$, column chromatography을 이용하여 TLC와 HPLC로 순도를 검증한 후 3 종의 polyphenol 화합물을 분리하였다. Compound I과 II는 Sepadex LH-20 gel column chromatography에서 증류수상에서 용출되었고 compound Ⅲ는 40% methanol 상에서 용출됨을 보아 compound II와 compound III는 흡착성이 강한 polyphenol 화합물이라 추정되었다. 혈압강하에 관여하는 효소인 angiotensin converting enzyme(ACE)의 저해효과는 compound II가 157 ppm에서 31.86%로 3 가지 분획물 중 가장 우수하였으며 compound I과 III는 높은 활성을 보이지 않았다. 통풍예방에 관여하는 xanthine oxidase 저해효과는 compound I, II에서 666 ppm에서 100%저해효과를 나타내었으나 compound III는 저해효과가 낮았다. Melanin을 형성하는 tyrosinase 저해효과를 실함한 결과 compound III는 400 ppm에서 28.6%의 저해효과를 나타내었고 Compound I과 II의 저해효과는 낮았다.

Keywords

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