Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Effect of Porphyran isolated from Laver, Porphyra yezoensis, on Liver Lipid Peroxidation in Hyperlipidemic Rats and on Immunological Functions in Mice

김 다당류 porphyran의 급이가 흰쥐의 혈청과 간의 효소활성 및 마우스의 면역에 미치는 영향

  • Jung, Kyoo-Jin (Department of Marine Food Industry, Provincial College of Namdo) ;
  • Jung, Bok-Mi (Department of Food Science and Nutrition, Yosu National University) ;
  • Kim, Seon-Bong (Department of Food and Biotechnology, Pukyong National University)
  • Published : 2002.04.01
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Abstract

This study was carried out to investigate the effect of porphyran on enzyme activity in rats and immunity in mice. Animals were divided into 5 groups, and were given porphyran diet for 4 weeks. Porphyran was extracted from Porphyra yezoensis: Diet groups were normal diet, control diet fed high fat, cholesterol and sodium cholate, control and 1% porphyran diet (1% PD), control and 5% porphyran diet (5% PD), control and 10% of porphyran diet (10% PD). Also Balb/c female mouse were injected i.p. with porphyran extract every other day for 20 days at levels of 1%, 2% and 5%. Alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP) and lactate dehydrogenase (LDH) activities were lower in the porphyran diet group than those in control group. Superoxide dismutase and catalase activities in liver homogenates were reduced in porphyran diet group compared to those of control group. Also, the level of liver thiobarbituric acid reactive substance (TBARS) was lower in porphyran group than that of control group. Porphyran increased IL-1 production in a dose-dependent manner, however, interleukine-2 production was reduced as the amount of porphyran increases. These results showed that supplementation of porphyran lowered antioxidant enzyme activities and has possibility of modulating immunological function.

남해안에서 다량 생산되고 있는 김(Porphyra yezoensis)의 생리활성 물질은 porphyran을 분리 추출한 후, 흰쥐에서 고지혈증 및 고 콜레스테롤혈증을 유발시켜 간장의 항산화 효소활성 및 면역능을 측정하였다. 간의 효소활성 측정을 위해 Sprague-Dawley계 흰쥐를 9마리씩 5군으로 나누어 1군은 정상군, 2군은 대조군(고지혈증 유발군), 3, 4 및 5군은 porphyran 급이군으로서 대조군에 porphyran을 각각 1%, 5% 및 10%의 비율로 사료에 첨가하여 4주간 사육하였다. 면역능은 Balb/c female mouse를 이용하여 3마리씩 4군으로 대조군 및 porphyran 1%, 2% 및 5%로 조제된 시료를 실험동물에게 2일에 1회 1mL씩 복강 주사를 연속적으로 20일간 투여한 후 비교하였다. 혈청중의 alanine aminotransferase(ALT), aspartate aminotransferase(AST), alkaline phosphatase(ALP) 및 lactic dehydrogenase(LDH) 활성은 대조군에 비해 porphyran 급이군에서 낮았으며, porphyran의 급이 수준이 증가할수록 대조군에 비해 유의하게 낮은 경향을 보였다. 간장의 superoxide dismutase와 catalase활성은 정상군에 비하여 대조군이 유의하게 높았으나, 대조군에 비해 porphyran 급이군에서는 낮았으며, 특히 10% porphyran 급이군에서는 정상군 수준으로 유의하게 감소되었다. TBARS 역시 대조군에 비해 porphyran 급이군 모두 유의하게 감소하였으며, 정상군 수준이하로 감소되었다. Interleukin-1농도는 porphyran 2% 및 5%를 투여한 흰쥐의 혈청에서 정상군보다 각각 26.81%, 77.95%의 증가를 나타내었으나, Interleukin-2는 감소하였다. 본 연구 결과에서 porphyran 급이는 고지혈증을 유발한 흰쥐에서 과산화물 농도를 저하시켜 항산화 효소활성을 낮추었으며, 면역능에서 일정한 현상은 나타나지 않았다.

Keywords

References

  1. Ministry of Maritime Affairs & Fisheries. Statistical year book of maritime affairs and fisheries, pp. 1169-1171 (2000)
  2. Jo, K.S., Do, J.R. and Koo, J.G. Pretreatment conditions of Por-phyra yezoensis, Undaria pinnatifida for functional algae-tea. J. Korean Soc. Food Sci. Nutr., 27: 275-280 (1998)
  3. 汁啓介 ,森文平 . 食物纖維の科學 , pp. 60-73.高分子水溶性食物纖維 . 朝倉書店 . 日 本 (1997)
  4. Kaneda, T. and Ando, H. Component lipids of purple laver and their antioxygenic activity. Proc. Int. Seaweed Symp. 7: 553-559(1971)
  5. Park, J.H., Kang, K.C., Baek, S.B., Lee, Y.H. and Rhee, K.S.Separation of antioxidant compounds from edible marine algae.Korean J. Food Sci. Technol. 23: 256-261 (1991)
  6. Park, Y.B., Kim, I.S., Yoo, S.J., Ahn, J.K., Lee, T.G., Park, D.C.and Kim, S.B. Elucidation of anti-tumor initiator and promoter derived from seaweed-2: Investigation of seaweed extracts Sup-pressing mutagenic activity of PhIP and MeIQx. J. Korean Fish. Soc. 31: 581-586 (1998)
  7. Noda, H., Amano, K. Arashima. Antitumour activity of polysac-charides and lipids from marine algae. Nippon Suisan Gakkaishi, 55(7): 1265-1271 (1989b) https://doi.org/10.2331/suisan.55.1265
  8. Cho, K.J., Lee, Y.S. and Ryu, B.H. Antitumor effect and immu-nological activity of seaweeds toward sarcoma-l8O. Bull. Korean Fish. Soc. 23: 345-352 (1990)
  9. 吉澤康子 , 野村和代 . 紅藻由來免疫賦浴 多糖に關する硏究 .食品工業 37: 25-30 (1994)
  10. Nishide, E., Ohno, M., Anzai, H. and Uchida, N. Extraction of porphyran from Porphyra yezoensis ueda F. narawaensis miura. Nippon Suisan Gakkaishi 54: 2189-2194 (1988) https://doi.org/10.2331/suisan.54.2189
  11. Koo, J.G. and Park, J.H. Chemical and gelling properties of alkali-modified porphyran. J. Korean Fish. Soc. 32: 271-275 (1999)
  12. Jung, K.J., Jung, B.M. and Kim, S.B. Effect of porphyran iso-lated from Laver, Porphyra yezoensis, on lipid metabolism in hyperlipidemic and hypercholesterolemic rats. Korean J. Food Sci.Technol 33: 633-640 (2001)
  13. Reitman, S. and Frankel, S. A colorimetric method for the deter-mination of serum glutamic oxaloacetic and glutamic pyruvic transaminase. Am. J. Clin. Pathol. 28: 56-63 (1957) https://doi.org/10.1093/ajcp/28.1.56
  14. Shah, S.V., Cruz, F.C., and Baricos, W.H. NADPH-induced chemiluminescence and lipid peroxidation on kidney microsomers. Kidney Int. 23: 691-698 (1983) https://doi.org/10.1038/ki.1983.80
  15. Stefan, M. and Gudrun, M. Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur. J. Biochem. 47: 469-474 (1974)
  16. Cohen, G., Dembiec, D. and Marcus, J. Measurement of catalase activity in tissue extracts. Agric. Biol. Chem. 34: 30-38 (1970)
  17. Lee, B.H., Choi, B.W., Chun, J.H. and Yu, B.S. Extraction of water soluble antioxidants from seaweeds. J. of Korean Ind. & Eng. Chem. 7: 1069 - 1077 (1996)
  18. Lokesh, B.R., Sayer, T.J. and Kinsella, J.E. Interleukin-1 and tumor necrosis factor synthesis by mouse peritoneal macrophage is enhanced by dietary n-3 polyunsaturated fatty acid. Immunol.Letter 23: 281-284 (1989)
  19. Meydani, S.N. Dietary modulation of cytokine production and biological functions. Nut. Rev. 48: 361-364 (1990) https://doi.org/10.1111/j.1753-4887.1990.tb02880.x
  20. Yoshizawa, Y, Ametani, A., Tsunehiro, J., Nomura, K., Itoh, M.,Fukui, F. and Kaminogawa, S. Macrophage stimulation activity of the polysccharide fraction from a marine algae (Porphyra yezoen-sis): stracture-function relationships and improved solubility. Bio-sci. Biotechnol. Biochem. 59: 1933-1937(1995) https://doi.org/10.1271/bbb.59.1933
  21. Yoshizawa, Y, Enomoto, A., Todoh, H., Ametani, A. and Kami-nogawa, S. Activation of murine macrophages by polysaccharide fractions marine algae (Porphyra yezoensis). Biosci. Biotechnol. Biochem. 57: 1862-1868 (1993) https://doi.org/10.1271/bbb.57.1862
  22. Yagoob, P. and Carder, P.C. The effect of fatty acids on lympho-cyte functions. Int. J. Biochem. 12: 1705-1709 (1993)
  23. Calder, P.C., Bond, J.A. Bevan, S.J. and Newsholeme, E.A.Unsaturated fatty acids inhibit interleukin-2 production by con-canavalin A-stimulated lymphocytes. Proc. Nutr. Soc. 50: 171-175(1991)
  24. Meydani, S.N. Modulation of cytokine production by dietary polyunsaturated fatty acids. P.S.E.B.M. 200: 189-191 (1992)
  25. Devi, M.A. and Das, N.P. Antiproliferlative effect of polyunsatu-rated fatty acid and interleukin-2 on normal and abnormal human lymphocytes. Res. Articles 50: 489-492 (1994)