Imaging Science in Dentistry

  • 제31권3호
  • /
  • Pages.135-143
  • /
  • 2001
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  • 2233-7822(pISSN)
  • /
  • 2233-7830(eISSN)
대한영상치의학회 (Korean Academy of Oral and Maxillofacial Radiology)
권호 표지

두경부 편평세포암종세포주에서 retinoic acid가 linear-quadratic 모델을 적용한 방사선감수성과 apoptosis 유발에 미치는 영향

The effect of retinoic acid on radiosensitivity analyzed by linear-quadratic model and apoptosis in head and neck squamous carcinoma cell lines

  • 이은숙 (서울대학교 치과대학 구강악안면방사선학교실, 두뇌한국21 및 치학연구소) ;
  • 강범현 (서울대학교 치과대학 구강악안면방사선학교실, 두뇌한국21 및 치학연구소) ;
  • 허민석 (서울대학교 치과대학 구강악안면방사선학교실, 두뇌한국21 및 치학연구소) ;
  • 이삼선 (서울대학교 치과대학 구강악안면방사선학교실, 두뇌한국21 및 치학연구소) ;
  • 최현배 (서울대학교 치과대학 구강악안면방사선학교실, 두뇌한국21 및 치학연구소) ;
  • 최순철 (서울대학교 치과대학 구강악안면방사선학교실, 두뇌한국21 및 치학연구소) ;
  • 박태원 (서울대학교 치과대학 구강악안면방사선학교실, 두뇌한국21 및 치학연구소)
  • Lee Eun-Sook (Department of Oral and Maxillofacial Radiology, BK21 & Dental Research Institute, College of Dentistry, Seoul National University) ;
  • Kang Bum-Hyun (Department of Oral and Maxillofacial Radiology, BK21 & Dental Research Institute, College of Dentistry, Seoul National University) ;
  • Heo Min-Suk (Department of Oral and Maxillofacial Radiology, BK21 & Dental Research Institute, College of Dentistry, Seoul National University) ;
  • Lee Sam-Sun (Department of Oral and Maxillofacial Radiology, BK21 & Dental Research Institute, College of Dentistry, Seoul National University) ;
  • Choi Hyun-Bae (Department of Oral and Maxillofacial Radiology, BK21 & Dental Research Institute, College of Dentistry, Seoul National University) ;
  • Choi Soon-Chul (Department of Oral and Maxillofacial Radiology, BK21 & Dental Research Institute, College of Dentistry, Seoul National University) ;
  • Park Tae-Won (Department of Oral and Maxillofacial Radiology, BK21 & Dental Research Institute, College of Dentistry, Seoul National University)
  • 발행 : 2001.09.01
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초록

Purpose : To evaluate the effect of all-trans-retinoic acid on radiosensitivity and radiation-induced apoptosis in NHOK, HEp-2 and FaDu cell lines. Material and Methods: We measured the changes in survival fraction at 2 Gy (SF2), α and β after treatment of retinoic acid (1μM) prior to irradiation with doses of 2, 4, 6 and 10 Gy and correlated the radiosensitizing effect of retinoic acid with them. Also, apoptosis induction was assayed with the flow cytometry on days 1,2, 3, 4 and 5 after irradiation (2, 10 and 20 Gy) combined with retinoic acid. Results and Conclusion: SF2 values for NHOK, HEp-2 and FaDu cell lines were 0.54, 0.64 and 0.41, respectively and the cell line of FaDu was the most radiosensitive. For cell lines of NHOK and HEp-2, pretreatment of cells with retinoic acid resulted in a significant decrease of the SF2 values. The α/β ratios of x-ray survival curve were 8.714 (NHOK), 4.098 (HEp-2) and 11.79 (FaDu). The α/β ratio for NHOK decreased on pretreatment with retinoic acid, whereas those for HEp-2 and FaDu increased. Radiation induced apoptosis in all cell lines but, retinoic acid did not affect the apoptosis.

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