The Journal of Korean Medicine (대한한의학회지)

The Society of Korean Medicine (대한한의학회)
권호 표지

The Effects of Artemisia capillaries Herbal Acupuncture on Ethanol-induced Apoptosis in Neuroblastoma Cell Line

인진 약침액이 신경아세포주에서 에탄올에 의해 유발된 아폽토시스에 미치는 영향

  • Ee-Hwa, Kim (College of Oriental Medicine, Semyung University) ;
  • Youn-Hee, Kim (Departments of Meridianology and Chemistry, Semyung University) ;
  • Youn-Jung, Kim (Departments of Physiology, College of Medicine, Kyunghee University) ;
  • Mi-Hyun, Jang (Departments of Physiology, College of Medicine, Kyunghee University) ;
  • Joo-Ho, Chung (Departments of Pharmacology, College of Medicine, Kyunghee University) ;
  • Chang-Ju, Kim (Departments of Physiology, College of Medicine, Kyunghee University)
  • Published : 2001.03.01
Download PDF
⟨ Previous Next ⟩

Abstract

목적：인진 약침액이 SK-N-MC 신경아세포주에서 에탄올에 의해 유발된 아폽토시스에 미치는 영향을 조사하였다. 방법 : SK-N-MC cell line에서의 아폽토시스 변화를 관찰하기 위해서 MTT assay, DAPI staining 및 flow cytometric analysis 방법을 이용하였다. 결과： MTT assay를 이용하여 분석한 결과 농도에 따른 세포 독성의 효과가 에탄올 투여로부터 관찰되었다. 또한 인진 약침액으로 전처치하고 에탄올을 처치하였을 때 세포 독성이 크게 감소되었다. DAPI staining에서 인진 약침액 투여군은 에탄올 투여군에 비해서 fragmentation이 억제되었다. Flow cytometry를 통하여 인진 약침액 투여군은 에탄올 투여군에 비하여 세포주기 중 sub $G_1$ 분획의 증가가 억제되었다. 결론 : SK-N-MC 신경아세포주에서 에탄올에 의해서 유발된 아폽토시스는 전형적인 세포사별 형태를 나타내었다. 또한 인진 약침액은 에탄올에 의해서 유발된 아폽토시스에서 세포보호 효과가 있음이 확인되었다.

Keywords

References

  1. Tetratology v.40 Differencial neuronal loss following postnatal alcohol exposure Pierce, D.R.;Goodlett, C.R.;West, J.R.
  2. Lancet v.2 Recognition of the fetal alcohol syndrome in early infancy Jones, K.L.;Smith, D.W.
  3. Neurotoxicology and Teratology v.19 no.2 Ethanol-induced cell death in cultured rat astroglia Holownia, A.;Ledig, M.;Menez, J.F.
  4. Developmental Brain Research v.119 Glial-derived neurotrophic factor(GDNF) prevents ethanol-induced apoptosis and JUN kinase phosphorylation McAlhany, R.E. Jr.;West, J.R.;Miranda, R.C.
  5. Journal of Hepatology v.20 Chronic ethanol feeding increases apoptosis and cell proliferation in rat liver Baroni, G.S.;Marucci, L.;Benedetti, A.;Mancini, R.;Jezequel, A.M.;Orlandi, F.
  6. Alcoholism Clinical and Experimental Research v.17 Ethanol increases apoptotic cell death of thymocytes in vitro Ewald, S.J.;Shao, H.
  7. Science v.287 Ethanol-induced apoptotic neurodegeneration and fetal alcohol syndrome Ikonomidou, C.;Bittigau, P.;Ishimaru, M.J.;Wozniak, D.F.;Koch, C.;Genz, K.;Price, M.T.;Strfovska, V.;Horster, F.;Tenkova, T.;Dikranian, K.;Olney, J.W.
  8. Science v.267 Apoptosis in the pathogenesis and treatment of disease Thompson, C.B.
  9. Chinese Drugs of Plant Origin, Chemistry, Pharmacology and Use in Traditional and Modern Medicine Tang, W.;Eisenbrand, G.
  10. Jpn. J. Cancer Res. v.91 no.1 Apoptosis in juman hepatoma cell line SMMC-7721 induced by water-soluble macromolecular components of Artemisia capillaries Thumberg Hu, Y.Q.;Tan, R.X.;Chu, M.Y.;Zhow, J.
  11. FEBS Letter v.439 Hypoxia induces apoptosis in juman neuroblastoma SK-N-MC cells by caspase activation accompanying cytochrome c release from mitochondria Araya, R.;Uehara, T.;Nomura, Y.
  12. Neuroreport v.10 Role of p53, c-myc, Bcl-2, bax and caspases in serum deprivation-induced neuronal apoptosis:a possible neuroprotective mechanism of basic fibroblast growth factor Liu, X.;Zhu, X.Z.
  13. Japanese Journal of Pharmacology v.84 Seritonin induces apoptosis in PGT-pineal gland tumor cells Yim, S.V.;Kim, K.W.;Kim, C.J.;Chung, J.H.