Korean Journal of Plant Tissue Culture (식물조직배양학회지)

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Effects of sucrose and MS salts in the addition of liquid medium on in vitro bulblet growth of Lilium oriental hybrid 'Casa Blanca'

액체배지 첨가시 sucrose 및 MS 염류가 Lilium Oriental Hybrid 'Casa Blanca' 의 기내 자구비대에 미치는 영향

  • 한봉희 (원예연구소) ;
  • 예병우 (원예연구소) ;
  • 백기엽 (충북대학교 원예학과 첨단원예기술개발연구센터) ;
  • 염미란 (충북대학교 원예학과 첨단원예기술개발연구센터)
  • Published : 2001.09.01
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Abstract

The effects of post-cultural addition of liquid medium addition to stimulate in vitro bulblet growth in Lilium oriental hybrid 'Casa Blanca' were investigated. The sections of bulblets with swollen basal plate (7 mm$\times$12 mm) were cultured on medium containing 60 g/L sucrose and 1 g/L activated charcoal for two months in dark, and then, liquid medium was added into the same vessels. The addition of liquid medium stimulated the growth of bulblets remarkably, compared to no addition of liquid medium. The liquid medium supplemented with 120 g/L sucrose and double strength of MS salts were the most effective on the growth of in vitro bulblets.

액체배지 첨가가 L. oriental hybrid 'Casa Blanca'의 자구비대 촉진에 미치는 영향을 조사하였다. MS기본배지에 활성탄 1 g/L과 sucrose 60g/L가 첨가된 배지에서 저반부가 비대된 자구절편체 (7 mm$\times$12 mm)를 2개월간 암배양한 후에 액체배지를 동일용기에 첨가하여 자구비대를 현저하게 촉진시켰다. 첨가된 액체배지는 2배의 MS배지 염류농도에 sucrose 120g/L가 첨가된 배지 25 mL를 첨가하는 것이 자구비대에 효과적이었다.

Keywords

References

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